ID   PPME1_DICDI             Reviewed;         321 AA.
AC   Q54TN3;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Probable protein phosphatase methylesterase 1;
DE            Short=PME-1;
DE            EC=3.1.1.89;
GN   Name=ppme1; ORFNames=DDB_G0281651;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Demethylates proteins that have been reversibly
CC       carboxymethylated. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester +
CC         H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) +
CC         methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-
CC         COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC         ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89;
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR   EMBL; AAFI02000042; EAL66587.1; -; Genomic_DNA.
DR   RefSeq; XP_640558.1; XM_635466.1.
DR   AlphaFoldDB; Q54TN3; -.
DR   SMR; Q54TN3; -.
DR   STRING; 44689.DDB0305014; -.
DR   ESTHER; dicdi-ppme1; PPase_methylesterase_euk.
DR   PaxDb; Q54TN3; -.
DR   EnsemblProtists; EAL66587; EAL66587; DDB_G0281651.
DR   GeneID; 8623168; -.
DR   KEGG; ddi:DDB_G0281651; -.
DR   dictyBase; DDB_G0281651; ppme1.
DR   eggNOG; KOG2564; Eukaryota.
DR   HOGENOM; CLU_024818_0_1_1; -.
DR   InParanoid; Q54TN3; -.
DR   OMA; WHIRSRT; -.
DR   PhylomeDB; Q54TN3; -.
DR   Reactome; R-DDI-69273; Cyclin A/B1/B2 associated events during G2/M transition.
DR   PRO; PR:Q54TN3; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0051722; F:protein C-terminal methylesterase activity; ISS:UniProtKB.
DR   GO; GO:0051723; F:protein methylesterase activity; IBA:GO_Central.
DR   GO; GO:0051721; F:protein phosphatase 2A binding; ISS:UniProtKB.
DR   GO; GO:0006482; P:protein demethylation; ISS:UniProtKB.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR016812; PPase_methylesterase_euk.
DR   PANTHER; PTHR14189; PTHR14189; 1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   PIRSF; PIRSF022950; PPase_methylesterase_euk; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00120; LIPASE_SER; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..321
FT                   /note="Probable protein phosphatase methylesterase 1"
FT                   /id="PRO_0000329315"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        195
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        307
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   321 AA;  36005 MW;  B9AB071A6E105A7E CRC64;
     MFRGIYKGSL PPIDPSSIHH GESLADQLQD KDYYSVGWNQ YFDQSRDIKL PGTENTFRIY
     ESNVDVVDNG YLFVFLHGGG YTSLSWSLVV DKIKKKNLEK KVRMMCYDCR GHGETKTSDD
     SNLSIETMVD DCANLINYYQ DIIYKNEGGG GGNDDDDQKE RLKVIIVGHS MGGSVVIKTS
     STNRINNLFG LIVIDVVEGT ALLALSSMKS ILAKRPKSFD SVKDAIKWSI SSNTVKNIES
     ARVSLYFINN FFFFFLTDWF SGLSKEFLSS MALKLLILAG TDRLDRELTI AQMQGKFQLI
     LLPLCGHVIQ EDVPTGPKIN I