ID   PPME1_KLULA             Reviewed;         395 AA.
AC   Q6CQZ5;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Protein phosphatase methylesterase 1;
DE            Short=PME-1;
DE            EC=3.1.1.89;
GN   Name=PPE1; OrderedLocusNames=KLLA0D13046g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Demethylates proteins that have been reversibly
CC       carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester +
CC         H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) +
CC         methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-
CC         COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC         ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89;
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR   EMBL; CR382124; CAH00740.1; -; Genomic_DNA.
DR   RefSeq; XP_453644.1; XM_453644.1.
DR   AlphaFoldDB; Q6CQZ5; -.
DR   SMR; Q6CQZ5; -.
DR   STRING; 28985.XP_453644.1; -.
DR   ESTHER; klula-ppme1; PPase_methylesterase_euk.
DR   EnsemblFungi; CAH00740; CAH00740; KLLA0_D13046g.
DR   GeneID; 2893320; -.
DR   KEGG; kla:KLLA0_D13046g; -.
DR   eggNOG; KOG2564; Eukaryota.
DR   HOGENOM; CLU_024818_3_0_1; -.
DR   InParanoid; Q6CQZ5; -.
DR   OMA; WHIRSRT; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005763; C:mitochondrial small ribosomal subunit; IEA:EnsemblFungi.
DR   GO; GO:0051723; F:protein methylesterase activity; IEA:InterPro.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:EnsemblFungi.
DR   GO; GO:1990577; P:C-terminal protein demethylation; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR016812; PPase_methylesterase_euk.
DR   PANTHER; PTHR14189; PTHR14189; 1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   PIRSF; PIRSF022950; PPase_methylesterase_euk; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..395
FT                   /note="Protein phosphatase methylesterase 1"
FT                   /id="PRO_0000223667"
FT   ACT_SITE        194
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        222
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        348
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   395 AA;  44181 MW;  7669EF91F5ED74E7 CRC64;
     MSGNLQREAL LKEFENAGKM IHAFDTAPEG NGEEDTITEL PVVKNPGLGV SSETKAENME
     DTGFPRWTEF FAKNETVSLP NRNFTFNTYF QVPEVNSSDL SSIPIFLAHH GAGSTGLTFA
     PLAKTLKEDL GTNFGFFSFD ARGHGETKPL DPTDVTYYLN DFVTDFVELI VWFYENYLKA
     CKQSKLSLIL VGHSLGGSVC ANLYEHLPEY IKRHTTGLAM LDIVEEMAKF VLTKVDHFLS
     VTPNVFGSAK EAVDWYQSHN YSKLKESAEI SVPALFHKAK SGKVVRITNL ASFKPYWDSW
     FDGLSAKFVS LPTSKLLILA GNDNLDKELI VGQMQGKYQL IVFQDSGHFI QEDVPKKAAI
     SLVDFWKRSD NRNVTIKTNW GSSQNKTLEG KKTVE