ATG10_ASHGO
ID ATG10_ASHGO Reviewed; 170 AA.
AC Q75CI5;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE EC=2.3.2.-;
DE AltName: Full=Autophagy-related protein 10;
GN Name=ATG10; OrderedLocusNames=ACL066C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 97.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC ATG5 is required for proper localization of ATG8 to the
CC preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC molecule (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms homooligomers. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR EMBL; AE016816; AAS51162.2; -; Genomic_DNA.
DR RefSeq; NP_983338.2; NM_208691.2.
DR AlphaFoldDB; Q75CI5; -.
DR SMR; Q75CI5; -.
DR EnsemblFungi; AAS51162; AAS51162; AGOS_ACL066C.
DR GeneID; 4619458; -.
DR KEGG; ago:AGOS_ACL066C; -.
DR HOGENOM; CLU_114192_0_0_1; -.
DR InParanoid; Q75CI5; -.
DR OMA; CDTDANV; -.
DR Proteomes; UP000000591; Chromosome III.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019777; F:Atg12 transferase activity; IEA:InterPro.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR016524; Atg10.
DR InterPro; IPR007135; Atg3/Atg10.
DR Pfam; PF03987; Autophagy_act_C; 1.
DR PIRSF; PIRSF007802; Autophagy-rel_ATG10; 1.
PE 3: Inferred from homology;
KW Autophagy; Membrane; Protein transport; Reference proteome; Transferase;
KW Transport; Ubl conjugation pathway.
FT CHAIN 1..170
FT /note="Ubiquitin-like-conjugating enzyme ATG10"
FT /id="PRO_0000096185"
FT ACT_SITE 123
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 170 AA; 19657 MW; A21E300AD09F7B5A CRC64;
MLSAEDFSDQ VLRLLPLLRR WRRCEHALWD PRARRLQLSV ATARNSARFD VRVDLHPLYA
VPQLLLRYWE PDPAVSEYEV WKLHFVDPYQ LPLWQPGRFS IALDQVPATS GSAYETWYVV
NSCDTDANVG PETDNYMLRW FSLYGQLLDE NLGFTLAAAL VAPCTDQKKA