ID   ATG10_CANAL             Reviewed;         177 AA.
AC   Q5AEI2; A0A1D8PJJ0;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
GN   Name=ATG10; OrderedLocusNames=CAALFM_C302930WA;
GN   ORFNames=CaO19.286, CaO19.7918;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN   [4]
RP   INDUCTION.
RX   PubMed=15273122; DOI=10.1128/aac.48.8.3064-3079.2004;
RA   Karababa M., Coste A.T., Rognon B., Bille J., Sanglard D.;
RT   "Comparison of gene expression profiles of Candida albicans azole-resistant
RT   clinical isolates and laboratory strains exposed to drugs inducing
RT   multidrug transporters.";
RL   Antimicrob. Agents Chemother. 48:3064-3079(2004).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC       that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC       ATG5 is required for proper localization of ATG8 to the
CC       preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC       molecule (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- INDUCTION: Expression is decreased upon fluphenazine treatment.
CC       {ECO:0000269|PubMed:15273122}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR   EMBL; CP017625; AOW28324.1; -; Genomic_DNA.
DR   RefSeq; XP_719983.1; XM_714890.1.
DR   AlphaFoldDB; Q5AEI2; -.
DR   GeneID; 3638380; -.
DR   KEGG; cal:CAALFM_C302930WA; -.
DR   CGD; CAL0000193637; orf19.7918.
DR   VEuPathDB; FungiDB:C3_02930W_A; -.
DR   eggNOG; ENOG502T5Z6; Eukaryota.
DR   HOGENOM; CLU_096164_0_0_1; -.
DR   InParanoid; Q5AEI2; -.
DR   OrthoDB; 1538225at2759; -.
DR   PRO; PR:Q5AEI2; -.
DR   Proteomes; UP000000559; Chromosome 3.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019787; F:ubiquitin-like protein transferase activity; IEA:InterPro.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Membrane; Protein transport; Reference proteome; Transferase;
KW   Transport; Ubl conjugation pathway.
FT   CHAIN           1..177
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000096186"
FT   ACT_SITE        135
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   177 AA;  20668 MW;  12EE99E1A5A5A072 CRC64;
     MITNQQFNDN IEEFISIMLA NLKLKGTDFE FSVNNNYVNK WNIDKMYSFV SVSVKQLRID
     FTISFDDLYG VPLLNMRLYD NDTFLTSPMA QRTLAVGICR VDLHNHHLLQ QPWLQVHPCE
     TLQTIDSHLK NTPTCKYNSV IQYLCCWFGL YGLPSIFPQF SVRPNIYINN VQSCKIK