ID   ATG10_CANGA             Reviewed;         159 AA.
AC   Q6FWS7;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
GN   Name=ATG10; OrderedLocusNames=CAGL0C03245g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC       that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC       ATG5 is required for proper localization of ATG8 to the
CC       preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC       molecule (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR   EMBL; CR380949; CAG58223.1; -; Genomic_DNA.
DR   RefSeq; XP_445317.1; XM_445317.1.
DR   AlphaFoldDB; Q6FWS7; -.
DR   SMR; Q6FWS7; -.
DR   STRING; 5478.XP_445317.1; -.
DR   EnsemblFungi; CAG58223; CAG58223; CAGL0C03245g.
DR   GeneID; 2886871; -.
DR   KEGG; cgr:CAGL0C03245g; -.
DR   CGD; CAL0127398; CAGL0C03245g.
DR   VEuPathDB; FungiDB:CAGL0C03245g; -.
DR   eggNOG; ENOG502S7IG; Eukaryota.
DR   HOGENOM; CLU_114192_0_0_1; -.
DR   InParanoid; Q6FWS7; -.
DR   Proteomes; UP000002428; Chromosome C.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019777; F:Atg12 transferase activity; IEA:EnsemblFungi.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IEA:EnsemblFungi.
DR   GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IEA:EnsemblFungi.
DR   GO; GO:0044805; P:late nucleophagy; IEA:EnsemblFungi.
DR   GO; GO:0016236; P:macroautophagy; IEA:EnsemblFungi.
DR   GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IEA:EnsemblFungi.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IEA:EnsemblFungi.
DR   InterPro; IPR016524; Atg10.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
DR   PIRSF; PIRSF007802; Autophagy-rel_ATG10; 1.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Protein transport; Reference proteome; Transferase;
KW   Transport; Ubl conjugation pathway.
FT   CHAIN           1..159
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000096187"
FT   ACT_SITE        129
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   159 AA;  18280 MW;  538C9E3B9514DD7B CRC64;
     MTLEYEQFVQ QLTRLAESRE LYKKLPLLRH DLVHSPCCLH LTVPNSTRNT GGHIELLITF
     SRVYREPLLL IRVWATTALD GIETSQLAHS AETMATLRIP SYLTLGLDTI LDAQYPHALQ
     GAWYSVHPCD TRDIVGDDVT VRDTYLDRWV SVFLLWVCR