ID   ATG10_GIBZE             Reviewed;         235 AA.
AC   A0A0E0RMV2; A0A1C3YHT0;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10 {ECO:0000250|UniProtKB:Q07879};
DE            EC=2.3.2.- {ECO:0000250|UniProtKB:Q07879};
DE   AltName: Full=Autophagy-related protein 10 {ECO:0000303|PubMed:28894236};
GN   Name=ATG10 {ECO:0000303|PubMed:28894236};
GN   ORFNames=FG00611, FGRAMPH1_01T01553;
OS   Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS   / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=229533;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=17823352; DOI=10.1126/science.1143708;
RA   Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA   Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA   Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA   Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA   Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA   Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA   Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA   Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT   "The Fusarium graminearum genome reveals a link between localized
RT   polymorphism and pathogen specialization.";
RL   Science 317:1400-1402(2007).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA   King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA   Hammond-Kosack K.E.;
RT   "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT   graminearum.";
RL   BMC Genomics 16:544-544(2015).
RN   [4]
RP   IDENTIFICATION, FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28894236; DOI=10.1038/s41598-017-11640-z;
RA   Lv W., Wang C., Yang N., Que Y., Talbot N.J., Wang Z.;
RT   "Genome-wide functional analysis reveals that autophagy is necessary for
RT   growth, sporulation, deoxynivalenol production and virulence in Fusarium
RT   graminearum.";
RL   Sci. Rep. 7:11062-11062(2017).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy (By similarity). Acts as an
CC       E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5 (By
CC       similarity). ATG12 conjugation to ATG5 is required for proper
CC       localization of ATG8 to the preautophagosomal structure (PAS) (By
CC       similarity). Likely serves as an ATG5-recognition molecule (By
CC       similarity). Autophagy is required for proper vegetative growth,
CC       asexual/sexual reproduction, and full virulence (PubMed:28894236).
CC       Autophagy is particularly involved in the biosynthesis of
CC       deoxynivalenol (DON), an important virulence determinant
CC       (PubMed:28894236). {ECO:0000250|UniProtKB:Q07879,
CC       ECO:0000269|PubMed:28894236}.
CC   -!- SUBUNIT: Forms homooligomers (By similarity). Interacts with ATG7 and
CC       ATG12 (By similarity). {ECO:0000250|UniProtKB:Q07879}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250|UniProtKB:Q07879}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q07879}.
CC   -!- DISRUPTION PHENOTYPE: Significantly decreases the radial growth of
CC       colonies under nutrient-rich conditions (PubMed:28894236).
CC       {ECO:0000269|PubMed:28894236}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=SCB64088.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HG970332; SCB64088.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; A0A0E0RMV2; -.
DR   STRING; 5518.FGSG_00611P0; -.
DR   eggNOG; KOG4741; Eukaryota.
DR   Proteomes; UP000070720; Chromosome 1.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019787; F:ubiquitin-like protein transferase activity; IEA:InterPro.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Protein transport; Reference proteome; Transferase;
KW   Transport; Ubl conjugation pathway.
FT   CHAIN           1..235
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000443899"
FT   ACT_SITE        196
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q07879"
SQ   SEQUENCE   235 AA;  26569 MW;  6ED2B2F0F04AAC7F CRC64;
     MSIENFPSLN SEEFTEACHH LDRQYCQAPL GSERARWKLR LCNALCTDFS YGGGFTTYVQ
     IRRPLEFDLD HGDLSLDLDG FSFSDEKGPH VSIAEDKDML DAEEADEAAL IRQRARPEIA
     MVEYEIHLHP TYRVPCLWFT LRNLPADEPA FDVDTVFRRL VPDEYKAGLR TLGNVGGISA
     DHHPITGVPS FFVHPCLLGD AISKFECDRT NYLMIWLGLV GGCVGLWVPK EMAMQ