PPOC_ORYSJ
ID PPOC_ORYSJ Reviewed; 536 AA.
AC Q9AR38; A0A0P0V168; Q5NAD7;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protoporphyrinogen oxidase, chloroplastic;
DE Short=PPO;
DE EC=1.3.3.4;
DE AltName: Full=Protoporphyrinogen oxidase 1;
DE Flags: Precursor;
GN Name=PPOX1; OrderedLocusNames=Os01g0286600, LOC_Os01g18320;
GN ORFNames=P0498A12.42;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Shoot;
RA Ichikawa H., Okano E., Sugita T., Shimizu T., Osakabe K., Toki S.;
RT "Characterization of a full length protoporphyrinogen oxidase I cDNA from
RT Oryza sativa L.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the 6-electron oxidation of protoporphyrinogen-IX
CC to form protoporphyrin-IX.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 O2 + protoporphyrinogen IX = 3 H2O2 + protoporphyrin IX;
CC Xref=Rhea:RHEA:25576, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:57306, ChEBI:CHEBI:57307; EC=1.3.3.4;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC Note=Binds 1 FAD per subunit. {ECO:0000250};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protoporphyrinogen/coproporphyrinogen
CC oxidase family. Protoporphyrinogen oxidase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD81569.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB057749; BAB39744.1; -; mRNA.
DR EMBL; AB057771; BAB39760.1; -; Genomic_DNA.
DR EMBL; AP003020; BAD81569.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAF04691.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71632.1; -; Genomic_DNA.
DR RefSeq; XP_015626054.1; XM_015770568.1.
DR AlphaFoldDB; Q9AR38; -.
DR SMR; Q9AR38; -.
DR STRING; 4530.OS01T0286600-01; -.
DR ChEMBL; CHEMBL2366510; -.
DR PaxDb; Q9AR38; -.
DR PRIDE; Q9AR38; -.
DR EnsemblPlants; Os01t0286600-01; Os01t0286600-01; Os01g0286600.
DR GeneID; 4327918; -.
DR Gramene; Os01t0286600-01; Os01t0286600-01; Os01g0286600.
DR KEGG; osa:4327918; -.
DR eggNOG; KOG1276; Eukaryota.
DR HOGENOM; CLU_009629_3_0_1; -.
DR InParanoid; Q9AR38; -.
DR OMA; WFDQWFG; -.
DR OrthoDB; 1578484at2759; -.
DR UniPathway; UPA00251; UER00324.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q9AR38; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProt.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0004729; F:oxygen-dependent protoporphyrinogen oxidase activity; IBA:GO_Central.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006783; P:heme biosynthetic process; IBA:GO_Central.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004572; Protoporphyrinogen_oxidase.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00562; proto_IX_ox; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; FAD; Flavoprotein;
KW Heme biosynthesis; Oxidoreductase; Plastid; Porphyrin biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..36
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 37..536
FT /note="Protoporphyrinogen oxidase, chloroplastic"
FT /id="PRO_0000376074"
FT REGION 248..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 62..67
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 87..88
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 111..114
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 510..512
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
SQ SEQUENCE 536 AA; 56727 MW; 25D9E3EDD49A97C3 CRC64;
MAAAAAAMAT ATSATAAPPL RIRDAARRTR RRGHVRCAVA SGAAEAPAAP GARVSADCVV
VGGGISGLCT AQALATKHGV GDVLVTEARA RPGGNITTAE RAGEGYLWEE GPNSFQPSDP
VLTMAVDSGL KDDLVFGDPN APRFVLWEGK LRPVPSKPGD LPFFDLMSIP GKLRAGLGAL
GVRAPPPGRE ESVEDFVRRN LGAEVFERLI EPFCSGVYAG DPSKLSMKAA FGKVWRLEDT
GGSIIGGTIK TIQERGKNPK PPRDPRLPTP KGQTVASFRK GLTMLPDAIT SRLGSKVKLS
WKLTSITKSD NKGYALVYET PEGVVSVQAK TVVMTIPSYV ASDILRPLSS DAADALSIFY
YPPVAAVTVS YPKEAIRKEC LIDGELQGFG QLHPRSQGVE TLGTIYSSSL FPNRAPAGRV
LLLNYIGGST NTGIVSKTES ELVEAVDRDL RKMLINPKAV DPLVLGVRVW PQAIPQFLIG
HLDHLEAAKS ALGKGGYDGL FLGGNYVAGV ALGRCVEGAY ESASQISDYL TKYAYK
