ID   ATG10_PICAN             Reviewed;         149 AA.
AC   A7KAI8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
GN   Name=ATG10;
OS   Pichia angusta (Yeast) (Hansenula polymorpha).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Pichiaceae; Ogataea.
OX   NCBI_TaxID=870730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC   Y-5445;
RX   PubMed=17204848; DOI=10.4161/auto.3595;
RA   Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W.;
RT   "ATG genes involved in non-selective autophagy are conserved from yeast to
RT   man, but the selective Cvt and pexophagy pathways also require organism-
RT   specific genes.";
RL   Autophagy 3:106-116(2007).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC       that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC       ATG5 is required for proper localization of ATG8 to the
CC       preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC       molecule (By similarity). {ECO:0000250, ECO:0000269|PubMed:17204848}.
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR   EMBL; EF102889; ABO31293.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7KAI8; -.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019787; F:ubiquitin-like protein transferase activity; IEA:InterPro.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Protein transport; Transferase; Transport;
KW   Ubl conjugation pathway.
FT   CHAIN           1..149
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000317921"
FT   ACT_SITE        120
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   149 AA;  17444 MW;  FF67F5E5A052BD0A CRC64;
     MKENVFENNV AQLAHYIRHK CTNVRTERTV IFALACRDNI QLEIVVFYHQ TYAAPLLTFR
     IWQLQVSDDI ETRRLVFDQT LIDKLVTRNG SLRVDRRVDP AITLVDHPVL GKPFYQLHPC
     QSAQLLEDVA LQGPESLEFW WNFYSSVLI