ID   ATG10_SCHPO             Reviewed;         179 AA.
AC   Q9UTD5;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
GN   Name=ATG10; ORFNames=SPAC227.04;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=23255127; DOI=10.4161/cc.23055;
RA   Flanagan M.D., Whitehall S.K., Morgan B.A.;
RT   "An Atg10-like E2 enzyme is essential for cell cycle progression but not
RT   autophagy in Schizosaccharomyces pombe.";
RL   Cell Cycle 12:271-277(2013).
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=23950735; DOI=10.1371/journal.pgen.1003715;
RA   Sun L.L., Li M., Suo F., Liu X.M., Shen E.Z., Yang B., Dong M.Q., He W.Z.,
RA   Du L.L.;
RT   "Global analysis of fission yeast mating genes reveals new autophagy
RT   factors.";
RL   PLoS Genet. 9:E1003715-E1003715(2013).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC       that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC       ATG5 is required for proper localization of ATG8 to the
CC       preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC       molecule. {ECO:0000269|PubMed:23255127, ECO:0000269|PubMed:23950735}.
CC   -!- SUBUNIT: Forms homooligomers. Interacts with ATG7 and ATG12.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       {ECO:0000269|PubMed:16823372}. Preautophagosomal structure membrane
CC       {ECO:0000269|PubMed:16823372}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB61453.1; -; Genomic_DNA.
DR   PIR; T50160; T50160.
DR   RefSeq; NP_592958.1; NM_001018358.2.
DR   AlphaFoldDB; Q9UTD5; -.
DR   BioGRID; 278403; 12.
DR   STRING; 4896.SPAC227.04.1; -.
DR   MaxQB; Q9UTD5; -.
DR   PaxDb; Q9UTD5; -.
DR   EnsemblFungi; SPAC227.04.1; SPAC227.04.1:pep; SPAC227.04.
DR   GeneID; 2541913; -.
DR   KEGG; spo:SPAC227.04; -.
DR   PomBase; SPAC227.04; -.
DR   VEuPathDB; FungiDB:SPAC227.04; -.
DR   eggNOG; ENOG502SBNV; Eukaryota.
DR   HOGENOM; CLU_1469036_0_0_1; -.
DR   InParanoid; Q9UTD5; -.
DR   OMA; AWIRFSP; -.
DR   Reactome; R-SPO-1632852; Macroautophagy.
DR   PRO; PR:Q9UTD5; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0061651; F:Atg12 conjugating enzyme activity; ISO:PomBase.
DR   GO; GO:0019777; F:Atg12 transferase activity; IBA:GO_Central.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IMP:PomBase.
DR   GO; GO:0016236; P:macroautophagy; IMP:PomBase.
DR   GO; GO:0006497; P:protein lipidation; IBA:GO_Central.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0070647; P:protein modification by small protein conjugation or removal; IC:PomBase.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   3: Inferred from homology;
KW   Autophagy; Cytoplasm; Membrane; Nucleus; Protein transport;
KW   Reference proteome; Transferase; Transport; Ubl conjugation pathway.
FT   CHAIN           1..179
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000310849"
FT   ACT_SITE        132
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   179 AA;  20626 MW;  B375F8D1E46E1D81 CRC64;
     MSFKSQLLIL AQKLSKGGIS CELIEFDECI LKLEWHTELL DKNDSLLYEQ EEDDILSLMN
     PMITMHAWIR DSPSFEVPQF FFQPYANGSD PLTKMEQIFE LLEGSSQNLA YDALAIGDCP
     GTVGIAWYIH PCRTRDYFEM LQIDKEDPKY LSLWLLYIHQ VLSPLTQPII KAVDDAEKS