ID   ATG10_VANPO             Reviewed;         196 AA.
AC   A7TNK5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG10;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 10;
GN   Name=ATG10; ORFNames=Kpol_1070p3;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: E2-like enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme
CC       that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to
CC       ATG5 is required for proper localization of ATG8 to the
CC       preautophagosomal structure (PAS). Likely serves as an ATG5-recognition
CC       molecule (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms homooligomers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG10 family. {ECO:0000305}.
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DR   EMBL; DS480432; EDO16122.1; -; Genomic_DNA.
DR   RefSeq; XP_001643980.1; XM_001643930.1.
DR   AlphaFoldDB; A7TNK5; -.
DR   SMR; A7TNK5; -.
DR   STRING; 436907.A7TNK5; -.
DR   EnsemblFungi; EDO16122; EDO16122; Kpol_1070p3.
DR   GeneID; 5544284; -.
DR   KEGG; vpo:Kpol_1070p3; -.
DR   eggNOG; ENOG502S7IG; Eukaryota.
DR   HOGENOM; CLU_114192_0_0_1; -.
DR   InParanoid; A7TNK5; -.
DR   OrthoDB; 1600752at2759; -.
DR   PhylomeDB; A7TNK5; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019787; F:ubiquitin-like protein transferase activity; IEA:InterPro.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   3: Inferred from homology;
KW   Autophagy; Membrane; Protein transport; Reference proteome; Transferase;
KW   Transport; Ubl conjugation pathway.
FT   CHAIN           1..196
FT                   /note="Ubiquitin-like-conjugating enzyme ATG10"
FT                   /id="PRO_0000317922"
FT   ACT_SITE        161
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   196 AA;  22816 MW;  0C7D84F84FDD4D90 CRC64;
     MITYEEYVKQ LKELDVYSTV AFKRVVPYVL IQQQQNEDEL LLRLRAPSEK LVGEINNGSN
     KLVIIDLKIS YSHVYQEPQL SIQLWETNGR QENQEEEQVD EIYLWYPNNI NETLAIPTNL
     FTIELDTVPR PRVERTTTDN INNSGSKKLM SSSAWYVVHS CDTSEIVGTP IQNYLQRWAS
     VYIASWWGIV RTPNNC