ID   PPR1B_BOVIN             Reviewed;         202 AA.
AC   P07516; Q17QN1;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Protein phosphatase 1 regulatory subunit 1B;
DE   AltName: Full=DARPP-32;
DE   AltName: Full=Dopamine- and cAMP-regulated neuronal phosphoprotein;
GN   Name=PPP1R1B;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   PROTEIN SEQUENCE, ACETYLATION AT MET-1, AND PHOSPHORYLATION AT THR-34.
RX   PubMed=3511054; DOI=10.1016/s0021-9258(17)36026-x;
RA   Williams K.R., Hemmings H.C. Jr., Lopresti M.B., Konigsberg W.H.,
RA   Greengard P.;
RT   "DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
RT   Primary structure and homology with protein phosphatase inhibitor-1.";
RL   J. Biol. Chem. 261:1890-1903(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3339428; DOI=10.1523/jneurosci.08-02-00508.1988;
RA   Kurihara T., Lewis R.M., Eisler J., Greengard P.;
RT   "Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated
RT   neuronal phosphoprotein.";
RL   J. Neurosci. 8:508-517(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Basal ganglia;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Inhibitor of protein-phosphatase 1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
CC   -!- PTM: Phosphorylation of Thr-34 is required for activity.
CC       {ECO:0000269|PubMed:3511054}.
CC   -!- SIMILARITY: Belongs to the protein phosphatase inhibitor 1 family.
CC       {ECO:0000305}.
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DR   EMBL; M27444; AAA57248.1; -; mRNA.
DR   EMBL; BC118264; AAI18265.1; -; mRNA.
DR   PIR; A45668; A26301.
DR   RefSeq; NP_777072.1; NM_174647.2.
DR   AlphaFoldDB; P07516; -.
DR   DIP; DIP-20N; -.
DR   STRING; 9913.ENSBTAP00000007930; -.
DR   iPTMnet; P07516; -.
DR   PaxDb; P07516; -.
DR   PRIDE; P07516; -.
DR   Ensembl; ENSBTAT00000007930; ENSBTAP00000007930; ENSBTAG00000006035.
DR   GeneID; 282459; -.
DR   KEGG; bta:282459; -.
DR   CTD; 84152; -.
DR   VEuPathDB; HostDB:ENSBTAG00000006035; -.
DR   VGNC; VGNC:33234; PPP1R1B.
DR   eggNOG; ENOG502S19Z; Eukaryota.
DR   GeneTree; ENSGT00730000111283; -.
DR   HOGENOM; CLU_092269_3_0_1; -.
DR   InParanoid; P07516; -.
DR   OrthoDB; 1412891at2759; -.
DR   TreeFam; TF332576; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000006035; Expressed in prostate gland and 104 other tissues.
DR   ExpressionAtlas; P07516; baseline and differential.
DR   GO; GO:1904115; C:axon cytoplasm; IDA:CAFA.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0032839; C:dendrite cytoplasm; IDA:CAFA.
DR   GO; GO:0043025; C:neuronal cell body; IDA:CAFA.
DR   GO; GO:0004864; F:protein phosphatase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR   DisProt; DP00421; -.
DR   InterPro; IPR015670; DARPP-32.
DR   InterPro; IPR008466; PPP1R1A/B/C.
DR   PANTHER; PTHR15417; PTHR15417; 1.
DR   PANTHER; PTHR15417:SF2; PTHR15417:SF2; 1.
DR   Pfam; PF05395; DARPP-32; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Direct protein sequencing; Phosphoprotein;
KW   Protein phosphatase inhibitor; Reference proteome.
FT   CHAIN           1..202
FT                   /note="Protein phosphatase 1 regulatory subunit 1B"
FT                   /id="PRO_0000071472"
FT   REGION          1..202
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        43..67
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        85..99
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        117..136
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000269|PubMed:3511054"
FT   MOD_RES         34
FT                   /note="Phosphothreonine; by PKA"
FT                   /evidence="ECO:0000269|PubMed:3511054"
FT   MOD_RES         45
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q60829"
FT   MOD_RES         46
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q60829"
FT   MOD_RES         75
FT                   /note="Phosphothreonine; by CDK5"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UD71"
FT   MOD_RES         102
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q60829"
FT   MOD_RES         135
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6J4I0"
SQ   SEQUENCE   202 AA;  22614 MW;  11192D2BED766763 CRC64;
     MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE ASPHQRASGE
     GHHLKSKRSN PCAYTPPSLK AVQRIAESHL QSISNLGENQ ASEEEDELGE LRELGYPREE
     EEEEEEEDEE EEEDSQAEVL KGSRGSAGQK TTYGQGLEGP WERPPPLDGP QRDGSSEDQV
     EDPALNEPGE EPQRPAHPEP GT