PPR1B_HUMAN
ID PPR1B_HUMAN Reviewed; 204 AA.
AC Q9UD71; Q547V9; Q547W0; Q9H7G1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 1B;
DE AltName: Full=DARPP-32;
DE AltName: Full=Dopamine- and cAMP-regulated neuronal phosphoprotein;
GN Name=PPP1R1B; Synonyms=DARPP32;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8120638; DOI=10.1523/jneurosci.14-03-00985.1994;
RA Brene S., Lindefors N., Ehrlich M., Taubes T., Horiuchi A., Kopp J.,
RA Hall H., Sedvall G., Greengard P., Persson H.;
RT "Expression of mRNAs encoding ARPP-16/19, ARPP-21, and DARPP-32 in human
RT brain tissue.";
RL J. Neurosci. 14:985-998(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=12124342;
RA El-Rifai W., Smith M.F. Jr., Li G., Beckler A., Carl V.S., Montgomery E.,
RA Knuutila S., Moskaluk C.A., Frierson H.F. Jr., Powell S.M.;
RT "Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP.";
RL Cancer Res. 62:4061-4064(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
RA Liu Q.-R., Uhl G.R.;
RT "A family of genes encoding PKC-dependent protein phosphatase 1 (PP1)
RT inhibitors.";
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Adipose tissue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PHOSPHORYLATION AT THR-75 BY CDK5.
RX PubMed=10604473; DOI=10.1038/45251;
RA Bibb J.A., Snyder G.L., Nishi A., Yan Z., Meijer L., Fienberg A.A.,
RA Tsai L.-H., Kwon Y.T., Girault J.-A., Czernik A.J., Huganir R.L.,
RA Hemmings H.C. Jr., Nairn A.C., Greengard P.;
RT "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in
RT neurons.";
RL Nature 402:669-671(1999).
CC -!- FUNCTION: Inhibitor of protein-phosphatase 1.
CC -!- INTERACTION:
CC Q9UD71; Q8WZ60: KLHL6; NbExp=3; IntAct=EBI-722119, EBI-6426464;
CC Q9UD71; Q12800: TFCP2; NbExp=3; IntAct=EBI-722119, EBI-717422;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UD71-1; Sequence=Displayed;
CC Name=2; Synonyms=t-DARPP;
CC IsoId=Q9UD71-2; Sequence=VSP_005117;
CC -!- PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
CC -!- PTM: Phosphorylation of Thr-34 is required for activity. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein phosphatase inhibitor 1 family.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/PPP1R1BID44096ch17q12.html";
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DR EMBL; AF464196; AAM76683.1; -; mRNA.
DR EMBL; AY070271; AAL59016.1; -; mRNA.
DR EMBL; AF435972; AAP35094.1; -; mRNA.
DR EMBL; AF435973; AAP35095.1; -; mRNA.
DR EMBL; AF435974; AAP35096.1; -; mRNA.
DR EMBL; AF435975; AAP35097.1; -; Genomic_DNA.
DR EMBL; AF435975; AAP35098.1; -; Genomic_DNA.
DR EMBL; AK024593; BAB14931.1; -; mRNA.
DR EMBL; BT007076; AAP35739.1; -; mRNA.
DR EMBL; BC001519; AAH01519.1; -; mRNA.
DR CCDS; CCDS11339.1; -. [Q9UD71-1]
DR CCDS; CCDS11340.1; -. [Q9UD71-2]
DR RefSeq; NP_001229393.1; NM_001242464.1. [Q9UD71-2]
DR RefSeq; NP_115568.2; NM_032192.3. [Q9UD71-1]
DR RefSeq; NP_852606.1; NM_181505.3. [Q9UD71-2]
DR RefSeq; XP_016880705.1; XM_017025216.1. [Q9UD71-1]
DR RefSeq; XP_016880706.1; XM_017025217.1. [Q9UD71-2]
DR AlphaFoldDB; Q9UD71; -.
DR BioGRID; 123915; 7.
DR ELM; Q9UD71; -.
DR IntAct; Q9UD71; 5.
DR STRING; 9606.ENSP00000254079; -.
DR iPTMnet; Q9UD71; -.
DR PhosphoSitePlus; Q9UD71; -.
DR BioMuta; PPP1R1B; -.
DR DMDM; 23831292; -.
DR EPD; Q9UD71; -.
DR jPOST; Q9UD71; -.
DR MassIVE; Q9UD71; -.
DR MaxQB; Q9UD71; -.
DR PaxDb; Q9UD71; -.
DR PeptideAtlas; Q9UD71; -.
DR PRIDE; Q9UD71; -.
DR ProteomicsDB; 84112; -. [Q9UD71-1]
DR ProteomicsDB; 84113; -. [Q9UD71-2]
DR Antibodypedia; 28294; 769 antibodies from 41 providers.
DR DNASU; 84152; -.
DR Ensembl; ENST00000254079.9; ENSP00000254079.4; ENSG00000131771.14. [Q9UD71-1]
DR Ensembl; ENST00000394265.5; ENSP00000377808.1; ENSG00000131771.14. [Q9UD71-2]
DR Ensembl; ENST00000394267.2; ENSP00000377810.2; ENSG00000131771.14. [Q9UD71-2]
DR Ensembl; ENST00000580825.5; ENSP00000462137.1; ENSG00000131771.14. [Q9UD71-1]
DR GeneID; 84152; -.
DR KEGG; hsa:84152; -.
DR MANE-Select; ENST00000254079.9; ENSP00000254079.4; NM_032192.4; NP_115568.2.
DR UCSC; uc002hrz.4; human. [Q9UD71-1]
DR CTD; 84152; -.
DR DisGeNET; 84152; -.
DR GeneCards; PPP1R1B; -.
DR HGNC; HGNC:9287; PPP1R1B.
DR HPA; ENSG00000131771; Group enriched (brain, choroid plexus, intestine, salivary gland).
DR MIM; 604399; gene.
DR neXtProt; NX_Q9UD71; -.
DR OpenTargets; ENSG00000131771; -.
DR PharmGKB; PA33639; -.
DR VEuPathDB; HostDB:ENSG00000131771; -.
DR eggNOG; ENOG502S19Z; Eukaryota.
DR GeneTree; ENSGT00730000111283; -.
DR InParanoid; Q9UD71; -.
DR OMA; EDPCEGD; -.
DR PhylomeDB; Q9UD71; -.
DR TreeFam; TF332576; -.
DR PathwayCommons; Q9UD71; -.
DR Reactome; R-HSA-180024; DARPP-32 events.
DR SignaLink; Q9UD71; -.
DR SIGNOR; Q9UD71; -.
DR BioGRID-ORCS; 84152; 17 hits in 1078 CRISPR screens.
DR ChiTaRS; PPP1R1B; human.
DR GeneWiki; PPP1R1B; -.
DR GenomeRNAi; 84152; -.
DR Pharos; Q9UD71; Tbio.
DR PRO; PR:Q9UD71; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9UD71; protein.
DR Bgee; ENSG00000131771; Expressed in putamen and 138 other tissues.
DR ExpressionAtlas; Q9UD71; baseline and differential.
DR Genevisible; Q9UD71; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR GO; GO:0004862; F:cAMP-dependent protein kinase inhibitor activity; TAS:Reactome.
DR GO; GO:0004860; F:protein kinase inhibitor activity; TAS:UniProtKB.
DR GO; GO:0004864; F:protein phosphatase inhibitor activity; IDA:CAFA.
DR GO; GO:0019888; F:protein phosphatase regulator activity; TAS:Reactome.
DR GO; GO:0048148; P:behavioral response to cocaine; IEA:Ensembl.
DR GO; GO:0071314; P:cellular response to cocaine; IEA:Ensembl.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0007626; P:locomotory behavior; IEA:Ensembl.
DR GO; GO:0007621; P:negative regulation of female receptivity; IEA:Ensembl.
DR GO; GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:CAFA.
DR GO; GO:0001975; P:response to amphetamine; IEA:Ensembl.
DR GO; GO:0043278; P:response to morphine; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; TAS:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:Ensembl.
DR GO; GO:0008542; P:visual learning; IEA:Ensembl.
DR InterPro; IPR015670; DARPP-32.
DR InterPro; IPR008466; PPP1R1A/B/C.
DR PANTHER; PTHR15417; PTHR15417; 1.
DR PANTHER; PTHR15417:SF2; PTHR15417:SF2; 1.
DR Pfam; PF05395; DARPP-32; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Phosphoprotein;
KW Protein phosphatase inhibitor; Reference proteome.
FT CHAIN 1..204
FT /note="Protein phosphatase 1 regulatory subunit 1B"
FT /id="PRO_0000071473"
FT REGION 1..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 85..99
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..138
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 166..181
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P07516"
FT MOD_RES 34
FT /note="Phosphothreonine; by PKA"
FT /evidence="ECO:0000250|UniProtKB:P07516"
FT MOD_RES 45
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q60829"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q60829"
FT MOD_RES 75
FT /note="Phosphothreonine; by CDK5"
FT /evidence="ECO:0000269|PubMed:10604473"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q60829"
FT MOD_RES 137
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6J4I0"
FT MOD_RES 198
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q60829"
FT VAR_SEQ 1..36
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12124342,
FT ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334,
FT ECO:0000303|Ref.3, ECO:0000303|Ref.5"
FT /id="VSP_005117"
FT CONFLICT 155..157
FT /note="CGQ -> RGL (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="T -> R (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 204 AA; 22963 MW; E1CE7DDD29D2D4FB CRC64;
MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE ASPHQRASGE
GHHLKSKRPN PCAYTPPSLK AVQRIAESHL QSISNLNENQ ASEEEDELGE LRELGYPREE
DEEEEEDDEE EEEEEDSQAE VLKVIRQSAG QKTTCGQGLE GPWERPPPLD ESERDGGSED
QVEDPALSEP GEEPQRPSPS EPGT
