PPR1_YEAST
ID PPR1_YEAST Reviewed; 904 AA.
AC P07272; D6VY16;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Pyrimidine pathway regulatory protein 1;
GN Name=PPR1; OrderedLocusNames=YLR014C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6096561; DOI=10.1016/s0022-2836(84)80002-9;
RA Kammerer B., Guyonvarch A., Hubert J.-C.;
RT "Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and
RT codon usage.";
RL J. Mol. Biol. 180:239-250(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-513.
RC STRAIN=ATCC 204508 / S288c;
RA Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E.;
RT "A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not
RT harbour PKC2.";
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 29-123.
RX PubMed=7958913; DOI=10.1101/gad.8.20.2504;
RA Marmorstein R., Harrison S.C.;
RT "Crystal structure of a PPR1-DNA complex: DNA recognition by proteins
RT containing a Zn2Cys6 binuclear cluster.";
RL Genes Dev. 8:2504-2512(1994).
CC -!- FUNCTION: Positive regulator of URA1 and URA3 expression.
CC -!- SUBUNIT: Binds DNA as a homodimer.
CC -!- SUBCELLULAR LOCATION: Nucleus.
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DR EMBL; X01739; CAA25876.1; -; Genomic_DNA.
DR EMBL; Z73186; CAA97536.1; -; Genomic_DNA.
DR EMBL; X90564; CAA62160.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09332.1; -; Genomic_DNA.
DR PIR; S05877; RGBYP1.
DR RefSeq; NP_013114.1; NM_001181901.1.
DR PDB; 1PYI; X-ray; 3.20 A; A/B=29-123.
DR PDBsum; 1PYI; -.
DR AlphaFoldDB; P07272; -.
DR SMR; P07272; -.
DR BioGRID; 31288; 109.
DR DIP; DIP-2126N; -.
DR IntAct; P07272; 3.
DR MINT; P07272; -.
DR STRING; 4932.YLR014C; -.
DR iPTMnet; P07272; -.
DR MaxQB; P07272; -.
DR PaxDb; P07272; -.
DR PRIDE; P07272; -.
DR EnsemblFungi; YLR014C_mRNA; YLR014C; YLR014C.
DR GeneID; 850701; -.
DR KEGG; sce:YLR014C; -.
DR SGD; S000004004; PPR1.
DR VEuPathDB; FungiDB:YLR014C; -.
DR eggNOG; ENOG502QR1M; Eukaryota.
DR HOGENOM; CLU_004517_1_1_1; -.
DR InParanoid; P07272; -.
DR OMA; WCVYSFD; -.
DR BioCyc; YEAST:G3O-32175-MON; -.
DR EvolutionaryTrace; P07272; -.
DR PRO; PR:P07272; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P07272; protein.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IDA:SGD.
DR GO; GO:1900399; P:positive regulation of pyrimidine nucleotide biosynthetic process; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Metal-binding; Nucleus;
KW Pyrimidine biosynthesis; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..904
FT /note="Pyrimidine pathway regulatory protein 1"
FT /id="PRO_0000114965"
FT DNA_BIND 34..61
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 883..904
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 37
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 51
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT BINDING 51
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 54
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT HELIX 35..40
FT /evidence="ECO:0007829|PDB:1PYI"
FT HELIX 52..56
FT /evidence="ECO:0007829|PDB:1PYI"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:1PYI"
FT TURN 66..68
FT /evidence="ECO:0007829|PDB:1PYI"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:1PYI"
FT HELIX 74..93
FT /evidence="ECO:0007829|PDB:1PYI"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:1PYI"
SQ SEQUENCE 904 AA; 102724 MW; 836B16CEE27B23BD CRC64;
MKQKKFNSKK SNRTDLSKRG DSPNIGISKS RTACKRCRLK KIKCDQEFPS CKRCAKLEVP
CVSLDPATGK DVPRSYVFFL EDRLAVMMRV LKEYGVDPTK IRGNIPATSD DEPFDLKKYS
SVSSLGEEGI LPHNGLLADY LVQKGNSMAS SAITSKSMAS PQTINVQRKE FLVNSKKQDG
SALLPETGSP MTSDARAEEL RRCNKEISAL GTMRESSFNS FLGDSSGISF AKLVFTATNF
RQDSGDDVLD EDIKQREQKY NGYAEAENNP HFDPLELPPR HAAEVMISRF FVDTNSQLPL
LHRELFLKKY FEPIYGPWNP NIALASDQTG INSAFEIPIT SAFSAHTEPK RENVTEKIDV
CSSVDVPWYD TWETSQKVNM RPIVELPTKF HIPYFFLNII FAIGHATQVL KSDITTVATY
KRRATKYIAS LFSSSDRLEA LAGTLLMVIY SIMRPNVPGV WYTMGSVLRL TVDLGLHSEK
INKNYDAFTR EIRRRLFWCV YSLDRQICSY FGRPFGIPEE SITTRYPSLL DDSFITLTNR
EIDDYSDLPS PNPSSKVIAL AMYKIRRIQA SIVRILYAPG AELPRRFMDL ESWRIETYNE
LERWFQVDVP KNFEMMNCKF NSIWFDLNYH YSKSILYGLS PKFPTLNDTA FKIVLDSTKG
TIDVFYNLCV NKKIGYTWVA VHNMFMTGMT YLYVNFYSKN NINDCQEKVS EYTEKVLIVL
KNLIGFCESA KTCYTSYKIL SSVVIKLKFM QINDAKGIFS DSNPLTSQAN RMSSYDKKTN
VLGFDDGTFD NKVFNRTNFE EKAPFDIPLD EFFTELEKHS NVSQFNTLDV SEGNQVINES
ASTNTSSALN CQSYTNNQDI MDILFQVTSG SVWDEFFVRS GNGNEGESSY DISKGKNSES
GGIF
