PPR21_ARATH
ID PPR21_ARATH Reviewed; 741 AA.
AC Q9LN01;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Pentatricopeptide repeat-containing protein At1g08070, chloroplastic {ECO:0000305};
DE AltName: Full=Protein ORGANELLE TRANSCRIPT PROCESSING 82 {ECO:0000303|PubMed:19845878};
DE Flags: Precursor;
GN Name=PCMP-H12; Synonyms=OTP82 {ECO:0000303|PubMed:19845878};
GN OrderedLocusNames=At1g08070; ORFNames=T6D22.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=10809006; DOI=10.1023/a:1006352315928;
RA Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT unique to plants.";
RL Plant Mol. Biol. 42:603-613(2000).
RN [4]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19845878; DOI=10.1111/j.1365-313x.2009.04059.x;
RA Okuda K., Hammani K., Tanz S.K., Peng L., Fukao Y., Myouga F.,
RA Motohashi R., Shinozaki K., Small I., Shikanai T.;
RT "The pentatricopeptide repeat protein OTP82 is required for RNA editing of
RT plastid ndhB and ndhG transcripts.";
RL Plant J. 61:339-349(2010).
RN [6]
RP INTERACTION WITH ORRM1.
RX PubMed=23487777; DOI=10.1073/pnas.1220162110;
RA Sun T., Germain A., Giloteaux L., Hammani K., Barkan A., Hanson M.R.,
RA Bentolila S.;
RT "An RNA recognition motif-containing protein is required for plastid RNA
RT editing in Arabidopsis and maize.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E1169-E1178(2013).
RN [7]
RP INTERACTION WITH VAR3/OZ1.
RX PubMed=25768119; DOI=10.1371/journal.pgen.1005028;
RA Sun T., Shi X., Friso G., Van Wijk K., Bentolila S., Hanson M.R.;
RT "A zinc finger motif-containing protein is essential for chloroplast RNA
RT editing.";
RL PLoS Genet. 11:E1005028-E1005028(2015).
CC -!- FUNCTION: Involved in multiple sites RNA editing events in
CC chloroplasts. Involved in the editing of the site 9 of ndhB (ndhB-9)
CC and site 1 of ndhG (ndhG-1) transcripts, which are two plastid-encoded
CC subunits of the chloroplast NAD(P)H dehydrogenase (NDH) complex. Not
CC essential for the activity of the NDH complex of the photosynthetic
CC electron transport chain. {ECO:0000269|PubMed:19845878}.
CC -!- SUBUNIT: Interacts with ORRM1 (PubMed:23487777). Interacts with
CC VAR3/OZ1 (PubMed:25768119). {ECO:0000269|PubMed:23487777,
CC ECO:0000269|PubMed:25768119}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19845878}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:19845878}.
CC -!- MISCELLANEOUS: The DYW motif is dispensable for editing activity in
CC vivo. {ECO:0000269|PubMed:19845878}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC026875; AAF79838.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28239.1; -; Genomic_DNA.
DR RefSeq; NP_172286.1; NM_100682.3.
DR AlphaFoldDB; Q9LN01; -.
DR SMR; Q9LN01; -.
DR BioGRID; 22566; 2.
DR STRING; 3702.AT1G08070.1; -.
DR PaxDb; Q9LN01; -.
DR PRIDE; Q9LN01; -.
DR EnsemblPlants; AT1G08070.1; AT1G08070.1; AT1G08070.
DR GeneID; 837325; -.
DR Gramene; AT1G08070.1; AT1G08070.1; AT1G08070.
DR KEGG; ath:AT1G08070; -.
DR Araport; AT1G08070; -.
DR TAIR; locus:2205200; AT1G08070.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_37_2_1; -.
DR InParanoid; Q9LN01; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9LN01; -.
DR PRO; PR:Q9LN01; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LN01; baseline and differential.
DR Genevisible; Q9LN01; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0031425; P:chloroplast RNA processing; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 5.
DR Pfam; PF12854; PPR_1; 1.
DR Pfam; PF13041; PPR_2; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR TIGRFAMs; TIGR00756; PPR; 5.
DR PROSITE; PS51375; PPR; 14.
PE 2: Evidence at transcript level;
KW Chloroplast; mRNA processing; Plastid; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN ?..741
FT /note="Pentatricopeptide repeat-containing protein
FT At1g08070, chloroplastic"
FT /id="PRO_0000342762"
FT REPEAT 98..132
FT /note="PPR 1"
FT REPEAT 133..167
FT /note="PPR 2"
FT REPEAT 168..202
FT /note="PPR 3"
FT REPEAT 203..229
FT /note="PPR 4"
FT REPEAT 230..264
FT /note="PPR 5"
FT REPEAT 265..299
FT /note="PPR 6"
FT REPEAT 300..330
FT /note="PPR 7"
FT REPEAT 331..365
FT /note="PPR 8"
FT REPEAT 366..396
FT /note="PPR 9"
FT REPEAT 403..433
FT /note="PPR 10"
FT REPEAT 434..468
FT /note="PPR 11"
FT REPEAT 469..499
FT /note="PPR 12"
FT REPEAT 505..535
FT /note="PPR 13"
FT REGION 540..615
FT /note="Type E motif"
FT REGION 616..646
FT /note="Type E(+) motif"
FT REGION 647..741
FT /note="Type DYW motif"
SQ SEQUENCE 741 AA; 83512 MW; 66FF9A0E1E814EBA CRC64;
MMLSCSPLTV PSSSYPFHFL PSSSDPPYDS IRNHPSLSLL HNCKTLQSLR IIHAQMIKIG
LHNTNYALSK LIEFCILSPH FEGLPYAISV FKTIQEPNLL IWNTMFRGHA LSSDPVSALK
LYVCMISLGL LPNSYTFPFV LKSCAKSKAF KEGQQIHGHV LKLGCDLDLY VHTSLISMYV
QNGRLEDAHK VFDKSPHRDV VSYTALIKGY ASRGYIENAQ KLFDEIPVKD VVSWNAMISG
YAETGNYKEA LELFKDMMKT NVRPDESTMV TVVSACAQSG SIELGRQVHL WIDDHGFGSN
LKIVNALIDL YSKCGELETA CGLFERLPYK DVISWNTLIG GYTHMNLYKE ALLLFQEMLR
SGETPNDVTM LSILPACAHL GAIDIGRWIH VYIDKRLKGV TNASSLRTSL IDMYAKCGDI
EAAHQVFNSI LHKSLSSWNA MIFGFAMHGR ADASFDLFSR MRKIGIQPDD ITFVGLLSAC
SHSGMLDLGR HIFRTMTQDY KMTPKLEHYG CMIDLLGHSG LFKEAEEMIN MMEMEPDGVI
WCSLLKACKM HGNVELGESF AENLIKIEPE NPGSYVLLSN IYASAGRWNE VAKTRALLND
KGMKKVPGCS SIEIDSVVHE FIIGDKFHPR NREIYGMLEE MEVLLEKAGF VPDTSEVLQE
MEEEWKEGAL RHHSEKLAIA FGLISTKPGT KLTIVKNLRV CRNCHEATKL ISKIYKREII
ARDRTRFHHF RDGVCSCNDY W
