PPR32_HUMAN
ID PPR32_HUMAN Reviewed; 425 AA.
AC Q7Z5V6; Q4G0P4; Q96M77;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 32 {ECO:0000305};
GN Name=PPP1R32 {ECO:0000312|HGNC:HGNC:28869}; Synonyms=C11orf66;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP THR-382.
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PPP1CA.
RX PubMed=19389623; DOI=10.1016/j.chembiol.2009.02.012;
RA Hendrickx A., Beullens M., Ceulemans H., Den Abt T., Van Eynde A.,
RA Nicolaescu E., Lesage B., Bollen M.;
RT "Docking motif-guided mapping of the interactome of protein phosphatase-
RT 1.";
RL Chem. Biol. 16:365-371(2009).
CC -!- SUBUNIT: Interacts with PPP1CA. {ECO:0000269|PubMed:19389623}.
CC -!- INTERACTION:
CC Q7Z5V6; P62136: PPP1CA; NbExp=3; IntAct=EBI-4311771, EBI-357253;
CC Q7Z5V6; P36873-2: PPP1CC; NbExp=4; IntAct=EBI-4311771, EBI-3964623;
CC Q7Z5V6-2; Q9ULX6: AKAP8L; NbExp=3; IntAct=EBI-12000762, EBI-357530;
CC Q7Z5V6-2; Q60I27: ALS2CL; NbExp=3; IntAct=EBI-12000762, EBI-12078276;
CC Q7Z5V6-2; Q86V38: ATN1; NbExp=3; IntAct=EBI-12000762, EBI-11954292;
CC Q7Z5V6-2; Q8N5M1: ATPAF2; NbExp=5; IntAct=EBI-12000762, EBI-1166928;
CC Q7Z5V6-2; Q9BPU9: B9D2; NbExp=3; IntAct=EBI-12000762, EBI-6958971;
CC Q7Z5V6-2; O95429: BAG4; NbExp=6; IntAct=EBI-12000762, EBI-2949658;
CC Q7Z5V6-2; O75934: BCAS2; NbExp=3; IntAct=EBI-12000762, EBI-1050106;
CC Q7Z5V6-2; Q5SWW7: C10orf55; NbExp=3; IntAct=EBI-12000762, EBI-12809220;
CC Q7Z5V6-2; Q8NEC5: CATSPER1; NbExp=3; IntAct=EBI-12000762, EBI-744545;
CC Q7Z5V6-2; Q2TAC2-2: CCDC57; NbExp=3; IntAct=EBI-12000762, EBI-10961624;
CC Q7Z5V6-2; Q9Y2V7: COG6; NbExp=3; IntAct=EBI-12000762, EBI-3866319;
CC Q7Z5V6-2; P10606: COX5B; NbExp=3; IntAct=EBI-12000762, EBI-1053725;
CC Q7Z5V6-2; P33240: CSTF2; NbExp=3; IntAct=EBI-12000762, EBI-711360;
CC Q7Z5V6-2; Q9GZU7: CTDSP1; NbExp=3; IntAct=EBI-12000762, EBI-751587;
CC Q7Z5V6-2; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-12000762, EBI-3867333;
CC Q7Z5V6-2; Q86UW9: DTX2; NbExp=3; IntAct=EBI-12000762, EBI-740376;
CC Q7Z5V6-2; A1KXE4-2: FAM168B; NbExp=3; IntAct=EBI-12000762, EBI-12193763;
CC Q7Z5V6-2; Q14192: FHL2; NbExp=3; IntAct=EBI-12000762, EBI-701903;
CC Q7Z5V6-2; Q13643: FHL3; NbExp=3; IntAct=EBI-12000762, EBI-741101;
CC Q7Z5V6-2; Q5TD97: FHL5; NbExp=3; IntAct=EBI-12000762, EBI-750641;
CC Q7Z5V6-2; O75344: FKBP6; NbExp=6; IntAct=EBI-12000762, EBI-744771;
CC Q7Z5V6-2; O43559: FRS3; NbExp=3; IntAct=EBI-12000762, EBI-725515;
CC Q7Z5V6-2; Q08379: GOLGA2; NbExp=3; IntAct=EBI-12000762, EBI-618309;
CC Q7Z5V6-2; A0A024R8L2: hCG_1987119; NbExp=3; IntAct=EBI-12000762, EBI-14103818;
CC Q7Z5V6-2; O14964: HGS; NbExp=6; IntAct=EBI-12000762, EBI-740220;
CC Q7Z5V6-2; P31943: HNRNPH1; NbExp=3; IntAct=EBI-12000762, EBI-351590;
CC Q7Z5V6-2; Q9NSC5: HOMER3; NbExp=3; IntAct=EBI-12000762, EBI-748420;
CC Q7Z5V6-2; P49639: HOXA1; NbExp=5; IntAct=EBI-12000762, EBI-740785;
CC Q7Z5V6-2; O75031: HSF2BP; NbExp=3; IntAct=EBI-12000762, EBI-7116203;
CC Q7Z5V6-2; Q0VD86: INCA1; NbExp=3; IntAct=EBI-12000762, EBI-6509505;
CC Q7Z5V6-2; Q7L273: KCTD9; NbExp=3; IntAct=EBI-12000762, EBI-4397613;
CC Q7Z5V6-2; Q5T749: KPRP; NbExp=3; IntAct=EBI-12000762, EBI-10981970;
CC Q7Z5V6-2; Q15323: KRT31; NbExp=3; IntAct=EBI-12000762, EBI-948001;
CC Q7Z5V6-2; Q52LG2: KRTAP13-2; NbExp=3; IntAct=EBI-12000762, EBI-11953846;
CC Q7Z5V6-2; Q3LI72: KRTAP19-5; NbExp=3; IntAct=EBI-12000762, EBI-1048945;
CC Q7Z5V6-2; Q3LI70: KRTAP19-6; NbExp=3; IntAct=EBI-12000762, EBI-12805508;
CC Q7Z5V6-2; Q9BYR8: KRTAP3-1; NbExp=3; IntAct=EBI-12000762, EBI-9996449;
CC Q7Z5V6-2; Q9BYR7: KRTAP3-2; NbExp=3; IntAct=EBI-12000762, EBI-751260;
CC Q7Z5V6-2; Q3LI66: KRTAP6-2; NbExp=5; IntAct=EBI-12000762, EBI-11962084;
CC Q7Z5V6-2; Q8IUC2: KRTAP8-1; NbExp=3; IntAct=EBI-12000762, EBI-10261141;
CC Q7Z5V6-2; Q14847-2: LASP1; NbExp=3; IntAct=EBI-12000762, EBI-9088686;
CC Q7Z5V6-2; P25791-3: LMO2; NbExp=3; IntAct=EBI-12000762, EBI-11959475;
CC Q7Z5V6-2; P61968: LMO4; NbExp=3; IntAct=EBI-12000762, EBI-2798728;
CC Q7Z5V6-2; Q8N6F8: METTL27; NbExp=3; IntAct=EBI-12000762, EBI-8487781;
CC Q7Z5V6-2; O43482: OIP5; NbExp=3; IntAct=EBI-12000762, EBI-536879;
CC Q7Z5V6-2; Q6GQQ9: OTUD7B; NbExp=3; IntAct=EBI-12000762, EBI-527784;
CC Q7Z5V6-2; Q99471: PFDN5; NbExp=3; IntAct=EBI-12000762, EBI-357275;
CC Q7Z5V6-2; O15496: PLA2G10; NbExp=3; IntAct=EBI-12000762, EBI-726466;
CC Q7Z5V6-2; Q58EX7: PLEKHG4; NbExp=3; IntAct=EBI-12000762, EBI-949255;
CC Q7Z5V6-2; Q494U1-3: PLEKHN1; NbExp=3; IntAct=EBI-12000762, EBI-12014286;
CC Q7Z5V6-2; P36873: PPP1CC; NbExp=3; IntAct=EBI-12000762, EBI-356283;
CC Q7Z5V6-2; Q9GZV8: PRDM14; NbExp=3; IntAct=EBI-12000762, EBI-3957793;
CC Q7Z5V6-2; P54646: PRKAA2; NbExp=3; IntAct=EBI-12000762, EBI-1383852;
CC Q7Z5V6-2; P47897: QARS1; NbExp=3; IntAct=EBI-12000762, EBI-347462;
CC Q7Z5V6-2; P57052: RBM11; NbExp=3; IntAct=EBI-12000762, EBI-741332;
CC Q7Z5V6-2; Q8HWS3: RFX6; NbExp=3; IntAct=EBI-12000762, EBI-746118;
CC Q7Z5V6-2; Q9UFD9: RIMBP3; NbExp=3; IntAct=EBI-12000762, EBI-10182375;
CC Q7Z5V6-2; Q8ND83: SLAIN1; NbExp=3; IntAct=EBI-12000762, EBI-10269374;
CC Q7Z5V6-2; Q8WW24: TEKT4; NbExp=6; IntAct=EBI-12000762, EBI-750487;
CC Q7Z5V6-2; Q92734: TFG; NbExp=3; IntAct=EBI-12000762, EBI-357061;
CC Q7Z5V6-2; Q96PF1: TGM7; NbExp=3; IntAct=EBI-12000762, EBI-12029034;
CC Q7Z5V6-2; Q13077: TRAF1; NbExp=3; IntAct=EBI-12000762, EBI-359224;
CC Q7Z5V6-2; Q12933: TRAF2; NbExp=3; IntAct=EBI-12000762, EBI-355744;
CC Q7Z5V6-2; Q96RU7: TRIB3; NbExp=3; IntAct=EBI-12000762, EBI-492476;
CC Q7Z5V6-2; Q86WV8: TSC1; NbExp=3; IntAct=EBI-12000762, EBI-12806590;
CC Q7Z5V6-2; Q9H9P5-5: UNKL; NbExp=3; IntAct=EBI-12000762, EBI-12817837;
CC Q7Z5V6-2; Q2NL98: VMAC; NbExp=3; IntAct=EBI-12000762, EBI-2803134;
CC Q7Z5V6-2; Q9NZC7-5: WWOX; NbExp=3; IntAct=EBI-12000762, EBI-12040603;
CC Q7Z5V6-2; Q9H0C1: ZMYND12; NbExp=3; IntAct=EBI-12000762, EBI-12030590;
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q66HR9}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q66HR9}. Note=Localized to the cilia of
CC polarized ependymal cells during development and at the adult stage.
CC Preferentially locates between the peripheral microtubules of the
CC axoneme and the ciliary membrane. {ECO:0000250|UniProtKB:Q66HR9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7Z5V6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z5V6-2; Sequence=VSP_022731;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK057333; BAB71432.1; -; mRNA.
DR EMBL; BC043246; AAH43246.1; -; mRNA.
DR EMBL; BC053995; AAH53995.1; -; mRNA.
DR CCDS; CCDS53641.1; -. [Q7Z5V6-2]
DR CCDS; CCDS8008.1; -. [Q7Z5V6-1]
DR RefSeq; NP_001164224.1; NM_001170753.1. [Q7Z5V6-2]
DR RefSeq; NP_659454.2; NM_145017.2. [Q7Z5V6-1]
DR AlphaFoldDB; Q7Z5V6; -.
DR BioGRID; 128618; 94.
DR IntAct; Q7Z5V6; 73.
DR STRING; 9606.ENSP00000344140; -.
DR iPTMnet; Q7Z5V6; -.
DR PhosphoSitePlus; Q7Z5V6; -.
DR BioMuta; PPP1R32; -.
DR DMDM; 74738757; -.
DR EPD; Q7Z5V6; -.
DR MassIVE; Q7Z5V6; -.
DR PaxDb; Q7Z5V6; -.
DR PeptideAtlas; Q7Z5V6; -.
DR PRIDE; Q7Z5V6; -.
DR ProteomicsDB; 69355; -. [Q7Z5V6-1]
DR ProteomicsDB; 69356; -. [Q7Z5V6-2]
DR Antibodypedia; 51662; 15 antibodies from 7 providers.
DR DNASU; 220004; -.
DR Ensembl; ENST00000338608.7; ENSP00000344140.2; ENSG00000162148.11. [Q7Z5V6-1]
DR Ensembl; ENST00000432063.6; ENSP00000391560.2; ENSG00000162148.11. [Q7Z5V6-2]
DR GeneID; 220004; -.
DR KEGG; hsa:220004; -.
DR MANE-Select; ENST00000338608.7; ENSP00000344140.2; NM_145017.3; NP_659454.2.
DR UCSC; uc001nru.3; human. [Q7Z5V6-1]
DR CTD; 220004; -.
DR GeneCards; PPP1R32; -.
DR HGNC; HGNC:28869; PPP1R32.
DR HPA; ENSG00000162148; Tissue enhanced (testis).
DR MIM; 619047; gene.
DR neXtProt; NX_Q7Z5V6; -.
DR OpenTargets; ENSG00000162148; -.
DR PharmGKB; PA144596485; -.
DR VEuPathDB; HostDB:ENSG00000162148; -.
DR eggNOG; ENOG502QR8X; Eukaryota.
DR GeneTree; ENSGT00390000003127; -.
DR HOGENOM; CLU_686162_0_0_1; -.
DR InParanoid; Q7Z5V6; -.
DR OMA; TTYNQRY; -.
DR PhylomeDB; Q7Z5V6; -.
DR TreeFam; TF328734; -.
DR PathwayCommons; Q7Z5V6; -.
DR SignaLink; Q7Z5V6; -.
DR BioGRID-ORCS; 220004; 9 hits in 1069 CRISPR screens.
DR ChiTaRS; PPP1R32; human.
DR GenomeRNAi; 220004; -.
DR Pharos; Q7Z5V6; Tdark.
DR PRO; PR:Q7Z5V6; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q7Z5V6; protein.
DR Bgee; ENSG00000162148; Expressed in right uterine tube and 99 other tissues.
DR ExpressionAtlas; Q7Z5V6; baseline and differential.
DR Genevisible; Q7Z5V6; HS.
DR GO; GO:0036064; C:ciliary basal body; IDA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0019902; F:phosphatase binding; IDA:UniProtKB.
DR InterPro; IPR031410; PPP1R32.
DR PANTHER; PTHR34349; PTHR34349; 1.
DR Pfam; PF15691; PPP1R32; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Reference proteome.
FT CHAIN 1..425
FT /note="Protein phosphatase 1 regulatory subunit 32"
FT /id="PRO_0000274377"
FT REGION 93..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 230..249
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022731"
FT VARIANT 238
FT /note="T -> N (in dbSNP:rs11230707)"
FT /id="VAR_030271"
FT VARIANT 382
FT /note="S -> T (in dbSNP:rs12787061)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030272"
FT CONFLICT 155
FT /note="H -> R (in Ref. 1; BAB71432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 425 AA; 47295 MW; 61020C5A4F14563F CRC64;
MMGKLPLGVV SPYVKMSSGG YTDPLKFYAT SYCTAYGRED FKPRVGSHVG TGYKSNFQPV
VSCQASLEAL DNPARGEQAQ DHFQSVASQS YRPLEVPDGK HPLPWSMRQT SSGYGREKPS
AGPPTKEVRK VHFDTQEHGP QAITGLEPRE VPLLHQQQGQ DPLERENFRH GPRFMTSEYN
SKYLRDPLDQ PDFLQKKSIG AKEGSGFTKQ SHQSPIVFQP PSQALPGDPA LLPGQSVTKS
DFLPKTHLHG DEFLPVLARG SKRETAFSRG NERILNPRVP PPCPEPSSVS HQQFQPLHRM
QQTNVALLGR ETVGKKEPTG FSLNNPMYVR SPCDPDRDQR YLTTYNQGYF ENIPKGLDQE
GWTRGGIQPQ MPGGYALSQP VSCMEATPNP MESLRHLHPH VGRTLTSADP FYQNTPHSSR
CVAHS
