PPR32_RAT
ID PPR32_RAT Reviewed; 425 AA.
AC Q66HR9; Q91XJ3; Q91XJ4;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 32 {ECO:0000305};
DE AltName: Full=Protein IIIG9;
GN Name=Ppp1r32 {ECO:0000312|RGD:708532};
GN Synonyms=Iiig9 {ECO:0000303|PubMed:15018803};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=15018803; DOI=10.1016/s1567-133x(01)00012-6;
RA Danielson P.E., Sautkulis L.N., Foye P.E., Hedlund P.B., Carson M.J.;
RT "A novel mRNA expressed along brain ventricles.";
RL Gene Expr. Patterns 1:83-88(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=28194645; DOI=10.1007/s12035-017-0434-5;
RA Cifuentes M., Baeza V., Arrabal P.M., Visser R., Grondona J.M.,
RA Saldivia N., Martinez F., Nualart F., Salazar K.;
RT "Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation
RT and Maturation of Ependymal Cells.";
RL Mol. Neurobiol. 55:1652-1664(2018).
CC -!- SUBUNIT: Interacts with PPP1CA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:28194645}. Cytoplasm {ECO:0000269|PubMed:28194645}.
CC Note=Localized to the cilia of polarized ependymal cells during
CC development and at the adult stage (PubMed:28194645). Preferentially
CC locates between the peripheral microtubules of the axoneme and the
CC ciliary membrane (PubMed:28194645). {ECO:0000269|PubMed:28194645}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=IIIG9L;
CC IsoId=Q66HR9-1; Sequence=Displayed;
CC Name=2; Synonyms=IIIG9S;
CC IsoId=Q66HR9-2; Sequence=VSP_022732;
CC -!- TISSUE SPECIFICITY: Expressed in brain, ovaries and testis
CC (PubMed:15018803). Expressed in the tracheal epithelium and in
CC secondary spermatocytes and spermatids present in the seminiferous
CC tubule (PubMed:28194645). Expressed in ependymal cells lining the
CC ventricular walls of the brain (PubMed:28194645).
CC {ECO:0000269|PubMed:15018803, ECO:0000269|PubMed:28194645}.
CC -!- DEVELOPMENTAL STAGE: At E17 expressed in cells of the ventricular wall
CC in the lateral and third ventricle and to cells in the neuronal
CC parenchyma. At PN1, mainly expressed in the walls of the lateral and
CC third ventricles with an apical polarization.
CC {ECO:0000269|PubMed:28194645}.
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DR EMBL; AY032664; AAK56500.1; -; mRNA.
DR EMBL; AY032665; AAK56501.1; -; mRNA.
DR EMBL; BC081718; AAH81718.1; -; mRNA.
DR RefSeq; NP_665729.1; NM_145786.1.
DR RefSeq; XP_006231078.1; XM_006231016.3. [Q66HR9-2]
DR RefSeq; XP_008758438.1; XM_008760216.2. [Q66HR9-1]
DR RefSeq; XP_017444309.1; XM_017588820.1. [Q66HR9-1]
DR AlphaFoldDB; Q66HR9; -.
DR STRING; 10116.ENSRNOP00000028003; -.
DR iPTMnet; Q66HR9; -.
DR PhosphoSitePlus; Q66HR9; -.
DR PaxDb; Q66HR9; -.
DR PRIDE; Q66HR9; -.
DR Ensembl; ENSRNOT00000028003; ENSRNOP00000028003; ENSRNOG00000020620. [Q66HR9-1]
DR GeneID; 252958; -.
DR KEGG; rno:252958; -.
DR UCSC; RGD:708532; rat. [Q66HR9-1]
DR CTD; 220004; -.
DR RGD; 708532; Ppp1r32.
DR eggNOG; ENOG502QR8X; Eukaryota.
DR GeneTree; ENSGT00390000003127; -.
DR HOGENOM; CLU_686162_0_0_1; -.
DR InParanoid; Q66HR9; -.
DR OMA; TTYNQRY; -.
DR OrthoDB; 854481at2759; -.
DR PhylomeDB; Q66HR9; -.
DR TreeFam; TF328734; -.
DR PRO; PR:Q66HR9; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000020620; Expressed in testis and 10 other tissues.
DR Genevisible; Q66HR9; RN.
DR GO; GO:0036064; C:ciliary basal body; ISO:RGD.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0019902; F:phosphatase binding; ISS:UniProtKB.
DR InterPro; IPR031410; PPP1R32.
DR PANTHER; PTHR34349; PTHR34349; 1.
DR Pfam; PF15691; PPP1R32; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Reference proteome.
FT CHAIN 1..425
FT /note="Protein phosphatase 1 regulatory subunit 32"
FT /id="PRO_0000274379"
FT REGION 259..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 315..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..297
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15018803"
FT /id="VSP_022732"
FT CONFLICT 31
FT /note="S -> C (in Ref. 1; AAK56501)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 425 AA; 47597 MW; 2618EF801A8FAAAF CRC64;
MMGKLPLGVV SPYVKMSSGG CSDPLKFYAT SYCTAYGREE FKPRMGSHVG TGYKSNYRPL
VSYQPHLDTL DNPAVGEQIH DTSKSVASQS YAPLEVPDGK QPLPWNLHQT TSGYGREKVN
IGPLSKEVRK VHFDTQDHGP QTITGLEPKE VPLIHQQQGK GSTEWENSRY GPRFMTSEYN
SKYLKESPNH PDLLLKKTIG SKEETGFTEE STKNPIVFQP PSQAYPGDPV LHPGRSITKS
DYLPISHPQG NEFLPVLARG SDRDTGYSRV SERSLNPRMP TPSSQPTSMS HRSYQPPQRM
QQSNVALLGR ESVGNKEPTG FTLNNPSYVR SSYEQDRDQR YLTTYNQGYF ENIPKGLDRE
GWTRGGIQPQ KAGAYALSEP VTHMDTTPNP TETLRHLHPH VGRTLTSVDP FYRDVPHSNR
YPTSS
