PPR35_DROME
ID PPR35_DROME Reviewed; 199 AA.
AC Q9VLM6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein PPP1R35 homolog {ECO:0000305};
DE AltName: Full=Protein Rcd4 {ECO:0000305};
DE AltName: Full=Reduction in Cnn dots 4 protein {ECO:0000312|FlyBase:FBgn0032034};
GN Name=Rcd4 {ECO:0000312|FlyBase:FBgn0032034};
GN ORFNames=CG17295 {ECO:0000312|FlyBase:FBgn0032034};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP ANA3.
RX PubMed=32543652; DOI=10.1083/jcb.201912154;
RA Panda P., Kovacs L., Dzhindzhev N., Fatalska A., Persico V., Geymonat M.,
RA Riparbelli M.G., Callaini G., Glover D.M.;
RT "Tissue specific requirement of Drosophila Rcd4 for centriole duplication
RT and ciliogenesis.";
RL J. Cell Biol. 219:0-0(2020).
CC -!- FUNCTION: Participates in the later stages of centriole assembly
CC through the interaction with Ana3 leading to the centriole to
CC centrosome conversion in somatic cells. {ECO:0000269|PubMed:32543652}.
CC -!- SUBUNIT: Interacts with Ana3; this complex is recruited to daughter
CC centrioles before their conversion to centrosomes.
CC {ECO:0000269|PubMed:32543652}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000269|PubMed:32543652}.
CC Note=Localizes to the more distal part of the lumen of the centriole.
CC Recruited into zone I during interphase after the formation of the
CC cartwheel at the end of mitosis (PubMed:32543652). The Rcd4 recruitment
CC to the daughter centriole is dependent on Ana3 (PubMed:32543652).
CC {ECO:0000269|PubMed:32543652}.
CC -!- DISRUPTION PHENOTYPE: Homozygous flies for rcd4 are lethal at the late
CC pupal and adult stages. The flies that emerged are slow and
CC uncoordinated. Female mutant flies are sterile. Male mutants are
CC fertile. {ECO:0000269|PubMed:32543652}.
CC -!- SIMILARITY: Belongs to the PPP1R35 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52659.1; -; Genomic_DNA.
DR EMBL; AE014134; ADV36972.1; -; Genomic_DNA.
DR EMBL; AY119223; AAM51083.1; -; mRNA.
DR RefSeq; NP_001188722.1; NM_001201793.1.
DR RefSeq; NP_609221.1; NM_135377.3.
DR AlphaFoldDB; Q9VLM6; -.
DR STRING; 7227.FBpp0079268; -.
DR PaxDb; Q9VLM6; -.
DR DNASU; 34158; -.
DR EnsemblMetazoa; FBtr0079652; FBpp0079268; FBgn0032034.
DR EnsemblMetazoa; FBtr0303472; FBpp0292524; FBgn0032034.
DR GeneID; 34158; -.
DR KEGG; dme:Dmel_CG17295; -.
DR UCSC; CG17295-RA; d. melanogaster.
DR CTD; 34158; -.
DR FlyBase; FBgn0032034; Rcd4.
DR VEuPathDB; VectorBase:FBgn0032034; -.
DR eggNOG; ENOG502TB9M; Eukaryota.
DR HOGENOM; CLU_1306049_0_0_1; -.
DR InParanoid; Q9VLM6; -.
DR OMA; VHANQRN; -.
DR OrthoDB; 1454086at2759; -.
DR PhylomeDB; Q9VLM6; -.
DR BioGRID-ORCS; 34158; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34158; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032034; Expressed in secondary oocyte and 24 other tissues.
DR GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0098534; P:centriole assembly; IMP:UniProtKB.
DR GO; GO:0007099; P:centriole replication; IMP:FlyBase.
DR InterPro; IPR029135; PPP1R35_C.
DR Pfam; PF15503; PPP1R35_C; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..199
FT /note="Protein PPP1R35 homolog"
FT /id="PRO_0000454386"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 199 AA; 22206 MW; DCE678C162E202CC CRC64;
MPHKRRNRVH ANQRNFTARR VSVAKLSSAP PNVLVESCNG SHADNSSPDS PKAKEGAMTN
GKNPEIKLAV PKCNTTVRKQ NEAHIISNIK LDMNLNKDSN FIAPQITKKM NFAPNRVVFG
VLVPLNVNDS VLVPHNRKPD ALKHTSKESE SCKAISEPQL ADYAEKVDPM IMAVKEPVLH
LDWEPGPFDF FGAYRRTYN
