ID   PPR3C_CLABA             Reviewed;         285 AA.
AC   T1SFR8;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Protein phosphatase 1 regulatory subunit 3C {ECO:0000255|PIRNR:PIRNR038207, ECO:0000303|PubMed:23948083};
GN   Name=PPP1R3C {ECO:0000303|PubMed:23948083};
OS   Clarias batrachus (Walking catfish) (Silurus batrachus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Clariidae; Clarias.
OX   NCBI_TaxID=59899 {ECO:0000312|EMBL:AGT20523.1};
RN   [1] {ECO:0000312|EMBL:AGT20523.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, AND PHYLOGENETIC
RP   ANALYSIS.
RX   PubMed=23948083; DOI=10.1016/j.gene.2013.07.042;
RA   Mohindra V., Tripathi R.K., Singh R.K., Lal K.K.;
RT   "Molecular characterization and expression analysis of PPP1R3C in hypoxia-
RT   tolerant Indian catfish, Clarias batrachus (Linnaeus, 1758) under
RT   hypoxia.";
RL   Gene 530:127-133(2013).
CC   -!- FUNCTION: Acts as a glycogen-targeting subunit for PP1 and regulates
CC       its activity. Activates glycogen synthase, reduces glycogen
CC       phosphorylase activity and limits glycogen breakdown.
CC       {ECO:0000250|UniProtKB:Q7TMB3}.
CC   -!- SUBUNIT: Interacts with PPP1CC catalytic subunit of PP1 and associates
CC       with glycogen. Forms complexes with glycogen phosphorylase, glycogen
CC       synthase and phosphorylase kinase which is necessary for its regulation
CC       of PP1 activity. {ECO:0000250|UniProtKB:Q7TMB3}.
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed in the examined tissues
CC       including brain, muscle, liver and spleen under normoxic condition. Its
CC       expression is higher in insulin sensitive tissues (liver and muscle)
CC       than in the brain and spleen. Significantly increased expression in the
CC       liver and muscle under short-term (1-12 hours) hypoxia exposure.
CC       Significantly increased expression after long-term (natural) hypoxia
CC       exposure in liver and spleen. No significant differences in expression
CC       in brain for any time periods. {ECO:0000269|PubMed:23948083}.
CC   -!- INDUCTION: Expression is up-regulated by hypoxia-inducible factor (HIF)
CC       under hypoxic conditions. {ECO:0000269|PubMed:23948083}.
CC   -!- DOMAIN: The N-terminal region is required for binding to PP1, the
CC       central region is required for binding to glycogen and the C-terminal
CC       region is required for binding to glycogen phosphorylase, glycogen
CC       synthase and phosphorylase kinase. {ECO:0000250|UniProtKB:Q7TMB3}.
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DR   EMBL; KC633817; AGT20523.1; -; mRNA.
DR   AlphaFoldDB; T1SFR8; -.
DR   SMR; T1SFR8; -.
DR   GO; GO:0019903; F:protein phosphatase binding; ISS:UniProtKB.
DR   GO; GO:0071456; P:cellular response to hypoxia; IEP:UniProtKB.
DR   GO; GO:0005978; P:glycogen biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0005977; P:glycogen metabolic process; ISS:UniProtKB.
DR   Gene3D; 2.60.40.2440; -; 1.
DR   InterPro; IPR005036; CBM21_dom.
DR   InterPro; IPR038175; CBM21_dom_sf.
DR   InterPro; IPR017434; Pase-1_reg-su_3B/C/D_met.
DR   InterPro; IPR030683; PP1_3C.
DR   PANTHER; PTHR12307:SF15; PTHR12307:SF15; 1.
DR   Pfam; PF03370; CBM_21; 1.
DR   PIRSF; PIRSF038207; PP1_GT_animal; 1.
DR   PROSITE; PS51159; CBM21; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glycogen metabolism.
FT   CHAIN           1..285
FT                   /note="Protein phosphatase 1 regulatory subunit 3C"
FT                   /id="PRO_0000436337"
FT   DOMAIN          133..241
FT                   /note="CBM21"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00491"
FT   MOTIF           72..75
FT                   /note="PP1-binding motif"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   285 AA;  32690 MW;  651684E57373B0EA CRC64;
     MPVDMAVQLY ITSSPPLHHR FLSSYKDYRV RNLISVNCKP LRPCINTKNT HNTSLNPPCS
     VWKAPEPKAK KKVVFADSKG MSLTAVRVFS PCENKKSDSQ VQFQLPKLEV ALKPVQSRIL
     AFRQPATEYM EFRKRLMKNS VCLESCTLQG HTLTGTIKVR NVSFEKSVQV RITFDSWENH
     RDIECTFLND VCGCRDTDTF SFIIEIPACV LPQDSVEFCV SYTCGGKTHW DNNNGKNYAL
     VTTHDEKKDK TKETDLLDPF RNQQKRNRFT ADWNSLMIGI RGPTW