PPR87_ARATH
ID PPR87_ARATH Reviewed; 408 AA.
AC Q8LE47; O80694;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 2.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Pentatricopeptide repeat-containing protein At1g61870, mitochondrial;
DE AltName: Full=Protein PENTATRICOPEPTIDE REPEAT 336;
DE Flags: Precursor;
GN Name=PPR336; OrderedLocusNames=At1g61870; ORFNames=F8K4.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=18054044; DOI=10.1016/j.jmb.2007.11.011;
RA Uyttewaal M., Mireau H., Rurek M., Hammani K., Arnal N., Quadrado M.,
RA Giege P.;
RT "PPR336 is associated with polysomes in plant mitochondria.";
RL J. Mol. Biol. 375:626-636(2008).
CC -!- FUNCTION: May be involved in translation through its association with
CC polysomes. Could bind RNA.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:15269332, ECO:0000269|PubMed:18054044}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC004392; AAC28506.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33898.1; -; Genomic_DNA.
DR EMBL; AF428391; AAL16159.1; -; mRNA.
DR EMBL; AY062858; AAL32936.1; -; mRNA.
DR EMBL; BT009692; AAP88326.1; -; mRNA.
DR EMBL; AY085627; AAM62848.1; -; mRNA.
DR PIR; T02133; T02133.
DR RefSeq; NP_564786.1; NM_104869.3.
DR AlphaFoldDB; Q8LE47; -.
DR SMR; Q8LE47; -.
DR BioGRID; 27707; 4.
DR IntAct; Q8LE47; 4.
DR STRING; 3702.AT1G61870.1; -.
DR iPTMnet; Q8LE47; -.
DR PaxDb; Q8LE47; -.
DR PRIDE; Q8LE47; -.
DR ProteomicsDB; 234798; -.
DR EnsemblPlants; AT1G61870.1; AT1G61870.1; AT1G61870.
DR GeneID; 842484; -.
DR Gramene; AT1G61870.1; AT1G61870.1; AT1G61870.
DR KEGG; ath:AT1G61870; -.
DR Araport; AT1G61870; -.
DR TAIR; locus:2036833; AT1G61870.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_10_3_1; -.
DR InParanoid; Q8LE47; -.
DR OMA; CHLIHGF; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q8LE47; -.
DR PRO; PR:Q8LE47; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LE47; baseline and differential.
DR Genevisible; Q8LE47; AT.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 3.
DR Pfam; PF13041; PPR_2; 2.
DR SUPFAM; SSF48452; SSF48452; 1.
DR TIGRFAMs; TIGR00756; PPR; 5.
DR PROSITE; PS51375; PPR; 8.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding;
KW Reference proteome; Repeat; RNA-binding; Transit peptide.
FT TRANSIT 1..26
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 27..408
FT /note="Pentatricopeptide repeat-containing protein
FT At1g61870, mitochondrial"
FT /id="PRO_0000342828"
FT REPEAT 115..149
FT /note="PPR 1"
FT REPEAT 150..185
FT /note="PPR 2"
FT REPEAT 186..220
FT /note="PPR 3"
FT REPEAT 221..255
FT /note="PPR 4"
FT REPEAT 256..290
FT /note="PPR 5"
FT REPEAT 291..325
FT /note="PPR 6"
FT REPEAT 326..360
FT /note="PPR 7"
FT REPEAT 361..391
FT /note="PPR 8"
FT CONFLICT 16
FT /note="H -> Y (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="S -> A (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="R -> H (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="K -> N (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 111..112
FT /note="PD -> H (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 250
FT /note="D -> A (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="R -> K (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
FT CONFLICT 317
FT /note="I -> V (in Ref. 4; AAM62848)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 408 AA; 45758 MW; F7175C7A1BC3F4C7 CRC64;
MALLSRIRSS TSLFRHLNAS PQIRSLSSAS TILSPDSKTP LTSKEKSKAA LSLLKSEKDP
DRILEICRAA SLTPDCRIDR IAFSAAVENL AEKKHFSAVS NLLDGFIENR PDLKSERFAA
HAIVLYAQAN MLDHSLRVFR DLEKFEISRT VKSLNALLFA CLVAKDYKEA KRVYIEMPKM
YGIEPDLETY NRMIKVFCES GSASSSYSIV AEMERKGIKP NSSSFGLMIS GFYAEDKSDE
VGKVLAMMKD RGVNIGVSTY NIRIQSLCKR KKSKEAKALL DGMLSAGMKP NTVTYSHLIH
GFCNEDDFEE AKKLFKIMVN RGCKPDSECY FTLIYYLCKG GDFETALSLC KESMEKNWVP
SFSIMKSLVN GLAKDSKVEE AKELIGQVKE KFTRNVELWN EVEAALPQ