PPR8_SCHPO
ID PPR8_SCHPO Reviewed; 481 AA.
AC O94615;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Pentatricopeptide repeat-containing protein 8, mitochondrial;
DE Flags: Precursor;
GN Name=ppr8; ORFNames=SPBC1289.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP DOMAIN, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=21727087; DOI=10.1093/nar/gkr511;
RA Kuhl I., Dujeancourt L., Gaisne M., Herbert C.J., Bonnefoy N.;
RT "A genome wide study in fission yeast reveals nine PPR proteins that
RT regulate mitochondrial gene expression.";
RL Nucleic Acids Res. 39:8029-8041(2011).
CC -!- FUNCTION: Mitochondrial RNA-binding protein involved in mitochondrial
CC translation. The cox1 mRNA is one target but it is not clear if ppr8
CC has a single or multiple targets. {ECO:0000269|PubMed:21727087}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372,
CC ECO:0000269|PubMed:21727087}.
CC -!- DISRUPTION PHENOTYPE: Exhibits growth arrest on glycerol medium.
CC {ECO:0000269|PubMed:21727087}.
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DR EMBL; CU329671; CAB38686.1; -; Genomic_DNA.
DR PIR; T39357; T39357.
DR RefSeq; NP_596830.1; NM_001023851.2.
DR AlphaFoldDB; O94615; -.
DR SMR; O94615; -.
DR BioGRID; 276161; 68.
DR STRING; 4896.SPBC1289.06c.1; -.
DR iPTMnet; O94615; -.
DR MaxQB; O94615; -.
DR PaxDb; O94615; -.
DR PRIDE; O94615; -.
DR EnsemblFungi; SPBC1289.06c.1; SPBC1289.06c.1:pep; SPBC1289.06c.
DR GeneID; 2539603; -.
DR KEGG; spo:SPBC1289.06c; -.
DR PomBase; SPBC1289.06c; ppr8.
DR VEuPathDB; FungiDB:SPBC1289.06c; -.
DR HOGENOM; CLU_567612_0_0_1; -.
DR InParanoid; O94615; -.
DR OMA; YHSEIIL; -.
DR PRO; PR:O94615; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005759; C:mitochondrial matrix; IC:PomBase.
DR GO; GO:0005739; C:mitochondrion; IDA:PomBase.
DR GO; GO:0140053; P:mitochondrial gene expression; ISM:PomBase.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF13812; PPR_3; 1.
PE 4: Predicted;
KW Activator; Mitochondrion; Protein biosynthesis; Reference proteome; Repeat;
KW Transit peptide; Translation regulation.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..481
FT /note="Pentatricopeptide repeat-containing protein 8,
FT mitochondrial"
FT /id="PRO_0000303930"
FT REPEAT 137..172
FT /note="PPR 1"
FT REPEAT 365..399
FT /note="PPR 2"
SQ SEQUENCE 481 AA; 54653 MW; 77DBC645E2781A38 CRC64;
MQGFGSQIFR KLLRSSNAKV SDALLQNTRT LFTAPPLHSG LQTSFTAETQ QHVRQNSQNL
LKQLNDEMKA RKYTETVATF SSLKPFGILD SQTINRYILF LVDRIKMLNG RGNVDEATLD
KLDDILRYAI EHQEIASARF WRIMLQSYID LNLFDKASLI ADMSLSHMEF LPKDERVLGN
LYISALQAKI LGGASFEQCG KIGSAIHEQL EGKEVVNELV AVYLIYTVFK DQGISSKAHK
ALVQFNGLTS FHSDVIISVF VNKGLVDQAA AYLKNSNLNE RNLPTIYTTV WLLQRLFEAH
HSLDPLLTIF DYYLSVSPKD ITRLTNAILS LSMKQFERDR DIKKATDFIT TFINKMSDVK
EFKPSISTAN TLFSIASRLK DVKWLSAGFD MIDKYGLKPT HVTYRSLLKA YCLLPSTCEQ
ISQAWVNLEY RLEAISISVA DKEINLLKDC ILSQPDRDDQ QSCLQFLNMV LSKYGKHIRS
P
