PPRB_PSEAE
ID PPRB_PSEAE Reviewed; 275 AA.
AC Q9HWA4;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Two-component response regulator PprB {ECO:0000303|PubMed:12499175};
GN Name=pprB {ECO:0000303|PubMed:12499175}; OrderedLocusNames=PA4296;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, AND PHOSPHORYLATION.
RC STRAIN=PAK;
RX PubMed=12499175; DOI=10.1128/aac.47.1.95-101.2003;
RA Wang Y., Ha U., Zeng L., Jin S.;
RT "Regulation of membrane permeability by a two-component regulatory system
RT in Pseudomonas aeruginosa.";
RL Antimicrob. Agents Chemother. 47:95-101(2003).
RN [3]
RP RETRACTED PAPER.
RX PubMed=15882421; DOI=10.1111/j.1365-2958.2005.04612.x;
RA Dong Y.H., Zhang X.F., Soo H.M., Greenberg E.P., Zhang L.H.;
RT "The two-component response regulator PprB modulates quorum-sensing signal
RT production and global gene expression in Pseudomonas aeruginosa.";
RL Mol. Microbiol. 56:1287-1301(2005).
RN [4]
RP RETRACTION NOTICE OF PUBMED:15882421.
RX PubMed=18684246; DOI=10.1111/j.1365-2958.2008.06351.x;
RA Pugsley A.;
RL Mol. Microbiol. 69:780-780(2008).
RN [5]
RP FUNCTION.
RX PubMed=19151143; DOI=10.1128/jb.01330-08;
RA Bernard C.S., Bordi C., Termine E., Filloux A., de Bentzmann S.;
RT "Organization and PprB-dependent control of the Pseudomonas aeruginosa tad
RT Locus, involved in Flp pilus biology.";
RL J. Bacteriol. 191:1961-1973(2009).
RN [6]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=21091863; DOI=10.1111/j.1462-2920.2010.02372.x;
RA Giraud C., Bernard C.S., Calderon V., Yang L., Filloux A., Molin S.,
RA Fichant G., Bordi C., de Bentzmann S.;
RT "The PprA-PprB two-component system activates CupE, the first non-
RT archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling
RT fimbriae.";
RL Environ. Microbiol. 13:666-683(2011).
RN [7]
RP FUNCTION.
RX PubMed=23209420; DOI=10.1371/journal.ppat.1003052;
RA de Bentzmann S., Giraud C., Bernard C.S., Calderon V., Ewald F.,
RA Plesiat P., Nguyen C., Grunwald D., Attree I., Jeannot K., Fauvarque M.O.,
RA Bordi C.;
RT "Unique biofilm signature, drug susceptibility and decreased virulence in
RT Drosophila through the Pseudomonas aeruginosa two-component system PprAB.";
RL PLoS Pathog. 8:E1003052-E1003052(2012).
RN [8]
RP FUNCTION, INDUCTION BY CARBON STARVATION, MUTAGENESIS OF ASP-60, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=31492668; DOI=10.1128/aem.01705-19;
RA Wang C., Chen W., Xia A., Zhang R., Huang Y., Yang S., Ni L., Jin F.;
RT "Carbon starvation induces the expression of PprB-regulated genes in
RT Pseudomonas aeruginosa.";
RL Appl. Environ. Microbiol. 0:0-0(2019).
CC -!- FUNCTION: Member of the two-component regulatory system PprA/PprB
CC involved in biofilm formation by controlling the expression of many
CC related genes including type IVb pili major subunit flp pilin, adhesin
CC bapA or cupE fimbriae (PubMed:19151143, PubMed:21091863,
CC PubMed:23209420, PubMed:31492668). Functions as a transcription
CC regulator by direct binding to promoter regions (PubMed:12499175,
CC PubMed:19151143). Negatively regulates its own transcription
CC (PubMed:31492668). {ECO:0000269|PubMed:12499175,
CC ECO:0000269|PubMed:19151143, ECO:0000269|PubMed:21091863,
CC ECO:0000269|PubMed:23209420, ECO:0000269|PubMed:31492668}.
CC -!- INDUCTION: By carbon starvation. This increased expression is
CC controlled by RpoS. {ECO:0000269|PubMed:31492668}.
CC -!- PTM: Phosphorylated by PprA. {ECO:0000269|PubMed:12499175}.
CC -!- DISRUPTION PHENOTYPE: Expression of flp in response to carbon
CC starvation stress is completely eliminated in the deletion mutant.
CC {ECO:0000269|PubMed:31492668}.
CC -!- CAUTION: An article reported the role of PprB in modulation of the
CC quorum-sensing signal production. However, this paper was later
CC retracted as the authors' main conclusion on the regulatory role of
CC PprB on AHL signal production in wild type P.aeruginosa is not correct.
CC {ECO:0000305|PubMed:15882421, ECO:0000305|PubMed:18684246}.
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DR EMBL; AE004091; AAG07684.1; -; Genomic_DNA.
DR PIR; G83107; G83107.
DR RefSeq; NP_252986.1; NC_002516.2.
DR RefSeq; WP_003114985.1; NZ_QZGE01000034.1.
DR AlphaFoldDB; Q9HWA4; -.
DR SMR; Q9HWA4; -.
DR STRING; 287.DR97_1471; -.
DR PaxDb; Q9HWA4; -.
DR PRIDE; Q9HWA4; -.
DR EnsemblBacteria; AAG07684; AAG07684; PA4296.
DR GeneID; 881658; -.
DR KEGG; pae:PA4296; -.
DR PATRIC; fig|208964.12.peg.4498; -.
DR PseudoCAP; PA4296; -.
DR HOGENOM; CLU_000445_90_4_6; -.
DR OMA; QIACELG; -.
DR PhylomeDB; Q9HWA4; -.
DR BioCyc; PAER208964:G1FZ6-4380-MON; -.
DR PHI-base; PHI:2620; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IDA:PseudoCAP.
DR GO; GO:0045785; P:positive regulation of cell adhesion; IMP:PseudoCAP.
DR GO; GO:1900233; P:positive regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..275
FT /note="Two-component response regulator PprB"
FT /id="PRO_0000448554"
FT DOMAIN 10..128
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 200..265
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 224..243
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT REGION 173..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MUTAGEN 60
FT /note="D->A: No change in transcriptional regulatory
FT activity."
FT /evidence="ECO:0000269|PubMed:31492668"
SQ SEQUENCE 275 AA; 30558 MW; 7C2DEB7CD823FCA5 CRC64;
MDKPASRHFS VLIIDDEPQV TSELRELLEN SGYRCVTSTH RESAIASFQA DPNIGLVICD
LYLGQDNGIR LIESLKEVAG NGRFFESIIL TGHDGRQEVI EAMRVGAADY YQKPVAPQEL
LHGLERLESR LHERVRSQLS LSHVNQRLEY LAESLNSIYR DIHKIKYEVH GNSQPSALRS
EDSQPSAPPA PVAESQVSPS NPLFGKLSPR QQAVARLVSK GLTNYQIAYE LGITENTVKL
YVSQVLRLMH MHNRTQLALA LSPAAMQQGS GAVVH
