ID   PPSC_ASPOR              Reviewed;         111 AA.
AC   Q2UAZ9;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=4'-hydroxy-3'-methoxypropiophenone carrier protein ppsC {ECO:0000303|PubMed:32885554};
DE   AltName: Full=2,4'-dihydroxy-3'-methoxypropiophenone biosynthesis cluster protein C;
GN   Name=ppsC {ECO:0000303|PubMed:32885554}; ORFNames=AO090102000167;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=32885554; DOI=10.1002/cbic.202000505;
RA   Kan E., Tomita H., Katsuyama Y., Maruyama J.I., Koyama Y., Ohnishi Y.;
RT   "Discovery of the 2,4'-dihydroxy-3'-methoxypropiophenone biosynthesis genes
RT   in Aspergillus oryzae.";
RL   ChemBioChem 22:203-211(2021).
CC   -!- FUNCTION: 4'-hydroxy-3'-methoxypropiophenone carrier protein; part of
CC       the gene cluster that mediates the biosynthesis of 2,4'-dihydroxy-3'-
CC       methoxypropiophenone (PubMed:32885554). The first step of the pathway
CC       is the conversion of acetate into acetyl-CoA by the acyl-CoA ligase
CC       ppsA (PubMed:32885554). Acetyl-CoA is then used as a starter unit by
CC       the polyketide synthase ppsB and condensed with 4 malonyl-CoA unit to
CC       produce the pentaketide backbone (PubMed:32885554). During polyketide
CC       extension, the polykedite chain is probably reduced and dehydrated by
CC       the KR and PT domains, respectively (Probable). O-methylation seems to
CC       be catalyzed by an unknown methyltransferase rather than by the CMeT
CC       domain of ppsB (Probable). Two hydroxylations and one further
CC       decarboxylation step catalyzed by yet unknown enzymes are then required
CC       to yield 4'-hydroxy-3'-methoxypropiophenone (Probable). PpsC functions
CC       as a carrier protein to transport 4'-hydroxy-3'-methoxypropiophenone to
CC       a specific cell compartment in which 4'-hydroxy-3'-methoxypropiophenone
CC       is hydroxylated to 2,4'-dihydroxy-3'-methoxypropiophenone by a still to
CC       be identified enzyme (PubMed:32885554). {ECO:0000269|PubMed:32885554,
CC       ECO:0000305|PubMed:32885554}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:32885554}.
CC   -!- DISRUPTION PHENOTYPE: Decreases the production of 2,4'-dihydroxy-3'-
CC       methoxypropiophenone and but leads to the accumulation of 4'-hydroxy-
CC       3'-methoxypropiophenone. {ECO:0000269|PubMed:32885554}.
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DR   EMBL; AP007162; BAE61266.1; -; Genomic_DNA.
DR   RefSeq; XP_001822399.2; XM_001822347.2.
DR   AlphaFoldDB; Q2UAZ9; -.
DR   EnsemblFungi; BAE61266; BAE61266; AO090102000167.
DR   GeneID; 5994444; -.
DR   KEGG; aor:AO090102000167; -.
DR   HOGENOM; CLU_109259_2_0_1; -.
DR   OMA; PLGFIVY; -.
DR   Proteomes; UP000006564; Chromosome 4.
DR   InterPro; IPR024311; Lipocalin-like.
DR   Pfam; PF13924; Lipocalin_5; 1.
PE   4: Predicted;
KW   Reference proteome.
FT   CHAIN           1..111
FT                   /note="4'-hydroxy-3'-methoxypropiophenone carrier protein
FT                   ppsC"
FT                   /id="PRO_0000451835"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   111 AA;  12639 MW;  414A45FFC1F1713D CRC64;
     MSAQVMRPGT PQHEGQEFLS GTDEELAVAM RHYLAYSGRF TVPDMASTEN STRRVIHEVE
     MSSYPNWIGT TQERVVHIKG DILELSTVHP LVISGIEQRS FLTWRKLPRL T