PPT2_ARATH
ID PPT2_ARATH Reviewed; 383 AA.
AC Q8H0T6; Q8LFI4; Q9SSA0;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Phosphoenolpyruvate/phosphate translocator 2, chloroplastic;
DE Short=AtPPT2;
DE Flags: Precursor;
GN Name=PPT2; OrderedLocusNames=At3g01550; ORFNames=F4P13.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14617097; DOI=10.1046/j.1365-313x.2003.01888.x;
RA Knappe S., Loettgert T., Schneider A., Voll L., Fluegge U.I., Fischer K.;
RT "Characterization of two functional phosphoenolpyruvate/phosphate
RT translocator (PPT) genes in Arabidopsis--AtPPT1 may be involved in the
RT provision of signals for correct mesophyll development.";
RL Plant J. 36:411-420(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Phosphoenolpyruvate/phosphate translocator that transports
CC phosphoenolpyruvate (PEP), 2-phosphoglycerate and 3-phosphoglycerate.
CC {ECO:0000269|PubMed:14617097}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely expressed in leaves throughout development.
CC In flowers, expressed in sepals and pistils.
CC {ECO:0000269|PubMed:14617097}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. PPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF01540.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009325; AAF01540.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73687.1; -; Genomic_DNA.
DR EMBL; BT002061; AAN72072.1; -; mRNA.
DR EMBL; BT008466; AAP37825.1; -; mRNA.
DR EMBL; AY084826; AAM61391.1; -; mRNA.
DR RefSeq; NP_566142.1; NM_111021.4.
DR AlphaFoldDB; Q8H0T6; -.
DR SMR; Q8H0T6; -.
DR BioGRID; 6447; 42.
DR IntAct; Q8H0T6; 42.
DR STRING; 3702.AT3G01550.1; -.
DR PaxDb; Q8H0T6; -.
DR ProteomicsDB; 234863; -.
DR EnsemblPlants; AT3G01550.1; AT3G01550.1; AT3G01550.
DR GeneID; 821114; -.
DR Gramene; AT3G01550.1; AT3G01550.1; AT3G01550.
DR KEGG; ath:AT3G01550; -.
DR Araport; AT3G01550; -.
DR TAIR; locus:2084203; AT3G01550.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_019048_0_0_1; -.
DR InParanoid; Q8H0T6; -.
DR OMA; MFATWYL; -.
DR OrthoDB; 1453018at2759; -.
DR PhylomeDB; Q8H0T6; -.
DR PRO; PR:Q8H0T6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8H0T6; baseline and differential.
DR Genevisible; Q8H0T6; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015121; F:phosphoenolpyruvate:phosphate antiporter activity; IDA:UniProtKB.
DR GO; GO:0015120; F:phosphoglycerate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015714; P:phosphoenolpyruvate transport; IDA:UniProtKB.
DR GO; GO:0015713; P:phosphoglycerate transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR InterPro; IPR004696; Tpt_PEP_transl.
DR Pfam; PF03151; TPT; 1.
DR TIGRFAMs; TIGR00817; tpt; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Pyruvate; Reference proteome;
KW Sugar transport; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 56..383
FT /note="Phosphoenolpyruvate/phosphate translocator 2,
FT chloroplastic"
FT /id="PRO_0000406100"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 93..212
FT /note="EamA"
FT CONFLICT 29
FT /note="Missing (in Ref. 4; AAM61391)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 42054 MW; B78A41A3B8E01CBA CRC64;
MFALTFLNPN PRLPSPLFLA KSTPESALSR RSRAFSSSNS YPWRPNLRFN GFKLKSATVP
ENVEGGDLES GSLVKGLKLG GMFGVWYLLN IYYNIFNKQV LRVYPYPATV TAFQLGCGTL
MIAIMWLLKL HPRPKFSPSQ FTVIVQLAVA HTLGNLLTNV SLGRVNVSFT HTIKAMEPFF
TVLLSVLLLG EWPSLWIVCS LLPIVAGVSL ASFTEASFNW IGFCSAMASN VTNQSRNVLS
KKFMVGKDAL DNINLFSIIT IISFILLVPL AILIDGFKVT PSHLQVATSQ GLSVKEFCIM
SLLAGVCLHS YQQVSYMILE MVSPVTHSVG NCVKRVVVIT SSILFFKTPV SPLNSIGTAT
ALAGVYLYSR AKRVQVKPNP KMS
