PPT2_ORYSJ
ID PPT2_ORYSJ Reviewed; 407 AA.
AC Q84QU8; A0A0P0XF61;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Phosphoenolpyruvate/phosphate translocator 2, chloroplastic;
DE Short=OsPPT2;
DE Flags: Precursor;
GN Name=PPT2; OrderedLocusNames=Os08g0344600, LOC_Os08g25624;
GN ORFNames=OJ1119_B10.35, P0410E11.104;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP FUNCTION.
RX PubMed=17981875; DOI=10.1093/pcp/pcm150;
RA Nozawa A., Nanamiya H., Miyata T., Linka N., Endo Y., Weber A.P.,
RA Tozawa Y.;
RT "A cell-free translation and proteoliposome reconstitution system for
RT functional analysis of plant solute transporters.";
RL Plant Cell Physiol. 48:1815-1820(2007).
CC -!- FUNCTION: Phosphoenolpyruvate/phosphate translocator that transports
CC phosphoenolpyruvate (PEP) and dihydroxyacetone phosphate.
CC {ECO:0000269|PubMed:17981875}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. PPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK066618; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AP004459; BAC75429.1; -; Genomic_DNA.
DR EMBL; AP004645; BAD03283.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23512.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05024.1; -; Genomic_DNA.
DR EMBL; AK066618; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650863.1; XM_015795377.1.
DR AlphaFoldDB; Q84QU8; -.
DR SMR; Q84QU8; -.
DR STRING; 4530.OS08T0344600-01; -.
DR PaxDb; Q84QU8; -.
DR PRIDE; Q84QU8; -.
DR EnsemblPlants; Os08t0344600-01; Os08t0344600-01; Os08g0344600.
DR GeneID; 4345334; -.
DR Gramene; Os08t0344600-01; Os08t0344600-01; Os08g0344600.
DR KEGG; osa:4345334; -.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_019048_0_0_1; -.
DR InParanoid; Q84QU8; -.
DR OMA; FWYTVSS; -.
DR OrthoDB; 1453018at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q84QU8; baseline and differential.
DR Genevisible; Q84QU8; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015121; F:phosphoenolpyruvate:phosphate antiporter activity; IDA:UniProtKB.
DR GO; GO:0009670; F:triose-phosphate:phosphate antiporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015714; P:phosphoenolpyruvate transport; IDA:UniProtKB.
DR GO; GO:0035436; P:triose phosphate transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR InterPro; IPR004696; Tpt_PEP_transl.
DR Pfam; PF03151; TPT; 1.
DR TIGRFAMs; TIGR00817; tpt; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Pyruvate; Reference proteome;
KW Sugar transport; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 52..407
FT /note="Phosphoenolpyruvate/phosphate translocator 2,
FT chloroplastic"
FT /id="PRO_0000406102"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 123..240
FT /note="EamA"
SQ SEQUENCE 407 AA; 43609 MW; CF0BF5F3D1414EA7 CRC64;
MQSAAAAFGL VRPCPARPPL QLGPGSSSCR PILLHARPLA AGIASSSRGP AAVAARSLGR
LLLLPPPPPI SPDRAGRGRA RHVACGAAAG DAKAEEEESG LAKTLQLGAL FGLWYLFNIY
FNIYNKQVLK VFPYPINITT VQFAVGTVVA LFMWITGILR RPKISGAQLF AILPLAVVHT
MGNLFTNMSL GKVAVSFTHT IKAMEPFFSV LLSAIFLGEL PTVWVILSLL PIVGGVALAS
LTEASFNWAG FWSAMASNVT FQSRNVLSKK LMVKKEESLD NINLFSIITV MSFFLLAPVA
FLTEGIKITP TVLQSAGLNV KQVLTRSLLA ALCFHAYQQV SYMILARVSP VTHSVGNCVK
RVVVIVTSVL FFRTPVSPIN SLGTAIALAG VFLYSQLKRL KPKPKAA