PPTA_ECOLI
ID PPTA_ECOLI Reviewed; 77 AA.
AC P31992;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Tautomerase PptA;
DE EC=5.3.2.-;
GN Name=pptA; Synonyms=ydcE; OrderedLocusNames=b1461, JW1456;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8387990; DOI=10.1128/jb.175.10.2799-2808.1993;
RA Zhao S., Sandt C.H., Feulner G., Vlazny D.A., Gray J.A., Hill C.W.;
RT "Rhs elements of Escherichia coli K-12: complex composites of shared and
RT unique components that have different evolutionary histories.";
RL J. Bacteriol. 175:2799-2808(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF NATIVE PROTEIN AND COMPLEX WITH
RP INHIBITOR, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES,
RP SUBUNIT, AND MASS SPECTROMETRY.
RC STRAIN=K12;
RX PubMed=12356301; DOI=10.1021/bi020271h;
RA Almrud J.J., Kern A.D., Wang S.C., Czerwinski R.M., Johnson W.H. Jr.,
RA Murzin A.G., Hackert M.L., Whitman C.P.;
RT "The crystal structure of YdcE, a 4-oxalocrotonate tautomerase homologue
RT from Escherichia coli, confirms the structural basis for oligomer
RT diversity.";
RL Biochemistry 41:12010-12024(2002).
CC -!- FUNCTION: Can use enol isomers of phenylpyruvate, 2-hydroxy-2,4-
CC pentadienoate and (p-hydroxyphenyl)pyruvate as substrates.
CC {ECO:0000269|PubMed:12356301}.
CC -!- ACTIVITY REGULATION: Inhibited by (E)-2-fluoro-p-hydroxycinnamate.
CC {ECO:0000269|PubMed:12356301}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=205 uM for enol isomers of phenylpyruvate
CC {ECO:0000269|PubMed:12356301};
CC KM=220 uM for 2-hydroxy-2,4-pentadienoate
CC {ECO:0000269|PubMed:12356301};
CC KM=180 uM for (p-hydroxyphenyl)pyruvate
CC {ECO:0000269|PubMed:12356301};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12356301}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=8540; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:12356301};
CC -!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family. PptA
CC subfamily. {ECO:0000305}.
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DR EMBL; X60998; CAA43312.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74543.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15092.1; -; Genomic_DNA.
DR PIR; H64898; H64898.
DR RefSeq; NP_415978.1; NC_000913.3.
DR RefSeq; WP_001120143.1; NZ_SSZK01000038.1.
DR PDB; 1GYJ; X-ray; 2.10 A; A/B=2-77.
DR PDB; 1GYX; X-ray; 1.35 A; A/B=2-77.
DR PDB; 1GYY; X-ray; 1.35 A; A/B=2-77.
DR PDBsum; 1GYJ; -.
DR PDBsum; 1GYX; -.
DR PDBsum; 1GYY; -.
DR AlphaFoldDB; P31992; -.
DR SMR; P31992; -.
DR BioGRID; 4262891; 27.
DR IntAct; P31992; 14.
DR STRING; 511145.b1461; -.
DR DrugBank; DB02384; (E)-2-Fluoro-P-Hydroxycinnamate.
DR jPOST; P31992; -.
DR PaxDb; P31992; -.
DR PRIDE; P31992; -.
DR EnsemblBacteria; AAC74543; AAC74543; b1461.
DR EnsemblBacteria; BAA15092; BAA15092; BAA15092.
DR GeneID; 945731; -.
DR KEGG; ecj:JW1456; -.
DR KEGG; eco:b1461; -.
DR PATRIC; fig|1411691.4.peg.807; -.
DR EchoBASE; EB1711; -.
DR eggNOG; COG1942; Bacteria.
DR HOGENOM; CLU_183611_0_1_6; -.
DR OMA; IKCFPRD; -.
DR BioCyc; EcoCyc:EG11761-MON; -.
DR BioCyc; MetaCyc:EG11761-MON; -.
DR SABIO-RK; P31992; -.
DR EvolutionaryTrace; P31992; -.
DR PRO; PR:P31992; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016862; F:intramolecular oxidoreductase activity, interconverting keto- and enol-groups; IDA:EcoCyc.
DR GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro.
DR Gene3D; 3.30.429.10; -; 1.
DR HAMAP; MF_00718; Tautomerase_PptA; 1.
DR InterPro; IPR004370; 4-oxalocrotonate_tautomerase.
DR InterPro; IPR014347; Tautomerase/MIF_sf.
DR InterPro; IPR017284; Tautomerase_PptA.
DR Pfam; PF01361; Tautomerase; 1.
DR PIRSF; PIRSF037799; Tautomer_YdcE_prd; 1.
DR SUPFAM; SSF55331; SSF55331; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Isomerase; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..77
FT /note="Tautomerase PptA"
FT /id="PRO_0000209526"
FT ACT_SITE 2
FT /note="Proton acceptor; via imino nitrogen"
FT STRAND 3..9
FT /evidence="ECO:0007829|PDB:1GYX"
FT HELIX 15..33
FT /evidence="ECO:0007829|PDB:1GYX"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:1GYX"
FT STRAND 41..46
FT /evidence="ECO:0007829|PDB:1GYX"
FT HELIX 49..51
FT /evidence="ECO:0007829|PDB:1GYX"
FT HELIX 52..58
FT /evidence="ECO:0007829|PDB:1GYX"
FT TURN 59..66
FT /evidence="ECO:0007829|PDB:1GYX"
SQ SEQUENCE 77 AA; 8673 MW; D32FF4023FE556CA CRC64;
MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE SWQAIWDAEI
APQMEALIKK PGYSMNA
