ID   PPZB_METRR              Reviewed;         483 AA.
AC   A0A166Z003;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   06-JUL-2016, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=MFS-type transporter ppzB {ECO:0000303|PubMed:30452111};
DE   AltName: Full=Pyrrolopyrazine biosynthesis cluster protein B {ECO:0000303|PubMed:30452111};
GN   Name=ppzB {ECO:0000303|PubMed:30452111}; ORFNames=NOR_07098;
OS   Metarhizium rileyi (strain RCEF 4871) (Nomuraea rileyi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=1081105;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RCEF 4871;
RX   PubMed=27071652; DOI=10.1093/gbe/evw082;
RA   Shang Y., Xiao G., Zheng P., Cen K., Zhan S., Wang C.;
RT   "Divergent and convergent evolution of fungal pathogenicity.";
RL   Genome Biol. Evol. 8:1374-1387(2016).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=30452111; DOI=10.1111/1462-2920.14483;
RA   Berry D., Mace W., Rehner S.A., Grage K., Dijkwel P.P., Young C.A.,
RA   Scott B.;
RT   "Orthologous peramine and pyrrolopyrazine-producing biosynthetic gene
RT   clusters in Metarhizium rileyi, Metarhizium majus and Cladonia grayi.";
RL   Environ. Microbiol. 21:928-939(2019).
CC   -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC       the biosynthesis of pyrrolopyrazines, secondary metabolites showing
CC       insecticidal activity (PubMed:30452111). Probably involved in the
CC       secretion of peramine and other pyrrolopyrazines (Probable).
CC       {ECO:0000269|PubMed:30452111, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; AZHC01000030; OAA37399.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A166Z003; -.
DR   SMR; A0A166Z003; -.
DR   STRING; 1081105.A0A166Z003; -.
DR   EnsemblFungi; OAA37399; OAA37399; NOR_07098.
DR   OrthoDB; 1371520at2759; -.
DR   Proteomes; UP000243498; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 2.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..483
FT                   /note="MFS-type transporter ppzB"
FT                   /id="PRO_0000450256"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..301
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        424..444
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        453..473
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        219
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   483 AA;  51735 MW;  6ABF1CCABDA94544 CRC64;
     MPDNHGPWGL RWRAKPSFIL TTVAMGLFTD LVLYGILLPA LPFTMRNRFD IPNAEIQHYT
     SAFLATYAGA SVFFSVPAGW AASKLGSRQL FLGGLMFLVV ATAIFAFSTS LVLLVVSRLL
     QGMSTAVVWT AGLDMVQDTV EPSQVGETIG TIFATISVGE LAAPVLGGVL YERGGISAVF
     AVSAVLLAID LALRALVIDK KTAVKYESPR LIRFPVERNM SDDHVASAPT VMEAQESVHE
     GTRLLPQVDD DGDHYKIDRE LGSIVRAIPL LYCFREPRLH LAMLLSFVQA LFIGTFDATV
     PTVAESLFHF SSLQVGLVFI ALMLPYFALG RLSGQAIDRF GTKAAATSGY AFLVPCLMLL
     GLPEKNLVPK EANVALFCTI LALNGIGLAV VTSPGYVEAI DVTTKYQVAN PGHFGENGPY
     AQLFGFSSLY FFTGLAVGPL LGGFLRANFG NAVMGAVYAA ISGVTAIVSF LFVGVRRFGP
     GLV