ID   PPZP_STRAQ              Reviewed;         299 AA.
AC   C4PWA1;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=5,10-dihydrophenazine-1-carboxylate 9-dimethylallyltransferase {ECO:0000305};
DE            Short=Dihydro-PCA dimethylallyltransferase {ECO:0000303|PubMed:19339241};
DE            EC=2.5.1.121 {ECO:0000269|PubMed:19339241};
DE   AltName: Full=Dihydro-PCA prenyltransferase {ECO:0000303|PubMed:19339241};
GN   Name=ppzP {ECO:0000303|PubMed:19339241};
OS   Streptomyces anulatus (Streptomyces chrysomallus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1892;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP   REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=9663;
RX   PubMed=19339241; DOI=10.1074/jbc.m901312200;
RA   Saleh O., Gust B., Boll B., Fiedler H.P., Heide L.;
RT   "Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-
RT   carboxylate dimethylallyltransferase from Streptomyces anulatus.";
RL   J. Biol. Chem. 284:14439-14447(2009).
CC   -!- FUNCTION: Involved in the biosynthesis of prenylated phenazines.
CC       Catalyzes the transfer of a dimethylallyl moiety to C-9 of 5,10-
CC       dihydrophenazine 1-carboxylate (dihydro-PCA). Specific for both
CC       dimethylallyl diphosphate and dihydro-PCA.
CC       {ECO:0000269|PubMed:19339241}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-dihydrophenazine 1-carboxylate + dimethylallyl
CC         diphosphate = 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate +
CC         diphosphate; Xref=Rhea:RHEA:41580, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57623, ChEBI:CHEBI:78312, ChEBI:CHEBI:78313;
CC         EC=2.5.1.121; Evidence={ECO:0000269|PubMed:19339241};
CC   -!- ACTIVITY REGULATION: Does not require magnesium or any other divalent
CC       metal ions for activity. {ECO:0000269|PubMed:19339241}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=116 uM for dimethylallyl diphosphate
CC         {ECO:0000269|PubMed:19339241};
CC         Note=kcat is 0.435 sec(-1). {ECO:0000269|PubMed:19339241};
CC   -!- PATHWAY: Antibiotic biosynthesis; phenazine biosynthesis.
CC       {ECO:0000269|PubMed:19339241}.
CC   -!- DISRUPTION PHENOTYPE: Mutant can form only nonprenylated phenazine 1-
CC       carboxylic acid. {ECO:0000269|PubMed:19339241}.
CC   -!- SIMILARITY: Belongs to the aromatic prenyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; FN178498; CAX48655.1; -; Genomic_DNA.
DR   AlphaFoldDB; C4PWA1; -.
DR   SMR; C4PWA1; -.
DR   KEGG; ag:CAX48655; -.
DR   BRENDA; 2.5.1.121; 5994.
DR   UniPathway; UPA00099; -.
DR   GO; GO:0004659; F:prenyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0002047; P:phenazine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd13931; PT-CloQ_NphB; 1.
DR   InterPro; IPR033964; Aro_prenylTrfase.
DR   InterPro; IPR020965; Prenyltransferase_CloQ.
DR   InterPro; IPR036239; PrenylTrfase-like_sf.
DR   Pfam; PF11468; PTase_Orf2; 1.
DR   SFLD; SFLDS00036; Aromatic_Prenyltransferase; 1.
DR   SFLD; SFLDG01163; II; 1.
DR   SUPFAM; SSF143492; SSF143492; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Prenyltransferase; Transferase.
FT   CHAIN           1..299
FT                   /note="5,10-dihydrophenazine-1-carboxylate 9-
FT                   dimethylallyltransferase"
FT                   /id="PRO_0000430678"
SQ   SEQUENCE   299 AA;  33012 MW;  E92778A36C604182 CRC64;
     MSESAELTEL YSAIEETTRV VGAPCRRDTV RPILTAYEDV IAQSVISFRV QTGTSDAGDL
     DCRFTLLPKD MDPYATALSN GLTAKTDHPV GSLLEEVHRQ FPVDCYGIDF GAVGGFKKAW
     SFFRPDSLQS ASDLAALPSM PSGVSENLGL FDRYGMTDTV SVVGFDYAKR SVNLYFTGAS
     PESFEPRGIQ AILRECGLPE PSDELLRFGE EAFAIYVTLS WDSQKIERVT YSVNTPDPMA
     LPVRIDTRIE QLVKDAPLGS AGHRYVYGVT ATPKGEYHKI QKYFQWQSRV EKMLTADAG