PQIA_ECOLI
ID PQIA_ECOLI Reviewed; 417 AA.
AC P0AFL9; P43670; P77566;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Intermembrane transport protein PqiA {ECO:0000305};
DE AltName: Full=Paraquat-inducible protein A;
GN Name=pqiA {ECO:0000303|PubMed:27795327};
GN Synonyms=pqi5A {ECO:0000303|PubMed:7751275};
GN OrderedLocusNames=b0950, JW0933;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7751275; DOI=10.1128/jb.177.10.2673-2678.1995;
RA Koh Y.-S., Roe J.-H.;
RT "Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS
RT locus in Escherichia coli.";
RL J. Bacteriol. 177:2673-2678(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=27795327; DOI=10.1128/jb.00606-16;
RA Nakayama T., Zhang-Akiyama Q.M.;
RT "pqiABC and yebST, putative mce operons of Escherichia coli, encode
RT transport pathways and contribute to membrane integrity.";
RL J. Bacteriol. 199:E00606-E00606(2017).
CC -!- FUNCTION: Component of a transport pathway that contributes to membrane
CC integrity. {ECO:0000269|PubMed:27795327}.
CC -!- SUBUNIT: May form a complex composed of PqiA, PqiB and PqiC.
CC {ECO:0000269|PubMed:27795327}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:27795327}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:27795327}.
CC -!- INDUCTION: By paraquat. {ECO:0000269|PubMed:7751275}.
CC -!- SIMILARITY: Belongs to the PqiA family. {ECO:0000305}.
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DR EMBL; X81561; CAA57256.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74036.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35708.1; -; Genomic_DNA.
DR PIR; E64835; E64835.
DR RefSeq; NP_415470.1; NC_000913.3.
DR RefSeq; WP_000333176.1; NZ_STEB01000006.1.
DR AlphaFoldDB; P0AFL9; -.
DR BioGRID; 4260027; 13.
DR IntAct; P0AFL9; 2.
DR STRING; 511145.b0950; -.
DR TCDB; 9.A.69.1.1; the intermembrane phospholipid translocase (impl-t) family.
DR PaxDb; P0AFL9; -.
DR PRIDE; P0AFL9; -.
DR EnsemblBacteria; AAC74036; AAC74036; b0950.
DR EnsemblBacteria; BAA35708; BAA35708; BAA35708.
DR GeneID; 66670774; -.
DR GeneID; 944999; -.
DR KEGG; ecj:JW0933; -.
DR KEGG; eco:b0950; -.
DR PATRIC; fig|1411691.4.peg.1324; -.
DR EchoBASE; EB2794; -.
DR eggNOG; COG2995; Bacteria.
DR HOGENOM; CLU_041903_0_1_6; -.
DR InParanoid; P0AFL9; -.
DR OMA; PWMMADV; -.
DR PhylomeDB; P0AFL9; -.
DR BioCyc; EcoCyc:G6490-MON; -.
DR PRO; PR:P0AFL9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR InterPro; IPR007498; PqiA-like.
DR InterPro; IPR005219; PqiA-like_Ent.
DR Pfam; PF04403; PqiA; 2.
DR TIGRFAMs; TIGR00155; pqiA_fam; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..417
FT /note="Intermembrane transport protein PqiA"
FT /id="PRO_0000058554"
FT TOPO_DOM 1..50
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..101
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..256
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..284
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..346
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..370
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT CONFLICT 338..417
FT /note="SERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALV
FT VIMTMFSAMTFDPRLSWDRQPESEHEES -> RKECI (in Ref. 1;
FT CAA57256)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 417 AA; 46463 MW; 959FD92C83F3BC8D CRC64;
MCEHHHAAKH ILCSQCDMLV ALPRLEHGQK AACPRCGTTL TVAWDAPRQR PTAYALAALF
MLLLSNLFPF VNMNVAGVTS EITLLEIPGV LFSEDYASLG TFFLLFVQLV PAFCLITILL
LVNRAELPVR LKEQLARVLF QLKTWGMAEI FLAGVLVSFV KLMAYGSIGV GSSFLPWCLF
CVLQLRAFQC VDRRWLWDDI APMPELRQPL KPGVTGIRQG LRSCSCCTAI LPADEPVCPR
CSTKGYVRRR NSLQWTLALL VTSIMLYLPA NILPIMVTDL LGSKMPSTIL AGVILLWSEG
SYPVAAVIFL ASIMVPTLKM IAIAWLCWDA KGHGKRDSER MHLIYEVVEF VGRWSMIDVF
VIAVLSALVR MGGLMSIYPA MGALMFALVV IMTMFSAMTF DPRLSWDRQP ESEHEES
