PQIB_ECOLI
ID PQIB_ECOLI Reviewed; 546 AA.
AC P43671; P77534;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Intermembrane transport protein PqiB {ECO:0000305};
DE AltName: Full=Paraquat-inducible protein B;
GN Name=pqiB {ECO:0000303|PubMed:27795327};
GN Synonyms=pqi5B {ECO:0000303|PubMed:7751275};
GN OrderedLocusNames=b0951, JW0934;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92, AND INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7751275; DOI=10.1128/jb.177.10.2673-2678.1995;
RA Koh Y.-S., Roe J.-H.;
RT "Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS
RT locus in Escherichia coli.";
RL J. Bacteriol. 177:2673-2678(1995).
RN [5]
RP FUNCTION, SUBUNIT, INTERACTION WITH PQIC, SUBCELLULAR LOCATION, AND
RP TOPOLOGY.
RX PubMed=27795327; DOI=10.1128/jb.00606-16;
RA Nakayama T., Zhang-Akiyama Q.M.;
RT "pqiABC and yebST, putative mce operons of Escherichia coli, encode
RT transport pathways and contribute to membrane integrity.";
RL J. Bacteriol. 199:E00606-E00606(2017).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.96 ANGSTROMS) OF 39-431, FUNCTION, AND
RP SUBUNIT.
RC STRAIN=K12;
RX PubMed=28388411; DOI=10.1016/j.cell.2017.03.019;
RA Ekiert D.C., Bhabha G., Isom G.L., Greenan G., Ovchinnikov S.,
RA Henderson I.R., Cox J.S., Vale R.D.;
RT "Architectures of lipid transport systems for the bacterial outer
RT membrane.";
RL Cell 169:273-285(2017).
CC -!- FUNCTION: Component of a transport pathway that contributes to membrane
CC integrity (PubMed:27795327). May directly span the intermembrane space,
CC facilitating the transport of substrates across the periplasm
CC (Probable). {ECO:0000269|PubMed:27795327, ECO:0000305|PubMed:28388411}.
CC -!- SUBUNIT: Homohexamer (PubMed:28388411). May form a complex composed of
CC PqiA, PqiB and PqiC. Interacts with PqiC (PubMed:27795327).
CC {ECO:0000269|PubMed:27795327, ECO:0000269|PubMed:28388411}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:27795327}; Single-pass membrane protein
CC {ECO:0000255}; Periplasmic side {ECO:0000269|PubMed:27795327}.
CC -!- INDUCTION: By paraquat. {ECO:0000269|PubMed:7751275}.
CC -!- SIMILARITY: Belongs to the PqiB family. {ECO:0000305}.
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DR EMBL; U00096; AAC74037.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35709.1; -; Genomic_DNA.
DR EMBL; X81561; CAA57257.1; -; Genomic_DNA.
DR PIR; F64835; F64835.
DR RefSeq; NP_415471.1; NC_000913.3.
DR RefSeq; WP_000445533.1; NZ_SSZK01000002.1.
DR PDB; 5UVN; EM; 3.96 A; A/B/C/D/E/F=39-431.
DR PDBsum; 5UVN; -.
DR AlphaFoldDB; P43671; -.
DR SMR; P43671; -.
DR BioGRID; 4260028; 5.
DR DIP; DIP-10555N; -.
DR IntAct; P43671; 4.
DR STRING; 511145.b0951; -.
DR TCDB; 9.A.69.1.1; the intermembrane phospholipid translocase (impl-t) family.
DR jPOST; P43671; -.
DR PaxDb; P43671; -.
DR PRIDE; P43671; -.
DR EnsemblBacteria; AAC74037; AAC74037; b0951.
DR EnsemblBacteria; BAA35709; BAA35709; BAA35709.
DR GeneID; 66670773; -.
DR GeneID; 945653; -.
DR KEGG; ecj:JW0934; -.
DR KEGG; eco:b0951; -.
DR PATRIC; fig|1411691.4.peg.1323; -.
DR EchoBASE; EB2795; -.
DR eggNOG; COG1463; Bacteria.
DR eggNOG; COG3008; Bacteria.
DR HOGENOM; CLU_018765_3_1_6; -.
DR InParanoid; P43671; -.
DR OMA; HHVEIKA; -.
DR PhylomeDB; P43671; -.
DR BioCyc; EcoCyc:G6491-MON; -.
DR PRO; PR:P43671; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0120009; P:intermembrane lipid transfer; IDA:EcoCyc.
DR InterPro; IPR003399; Mce/MlaD.
DR Pfam; PF02470; MlaD; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..546
FT /note="Intermembrane transport protein PqiB"
FT /id="PRO_0000058557"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27795327"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..546
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:27795327"
FT REGION 42..133
FT /note="MCE/MlaD 1"
FT /evidence="ECO:0000305"
FT REGION 158..217
FT /note="MCE/MlaD 2"
FT /evidence="ECO:0000305"
FT REGION 285..389
FT /note="MCE/MlaD 3"
FT /evidence="ECO:0000305"
FT COILED 437..464
FT /evidence="ECO:0000255"
SQ SEQUENCE 546 AA; 60520 MW; 2C75CEB22AB8F7AB CRC64;
MESNNGEAKI QKVKNWSPVW IFPIVTALIG AWVLFYHYSH QGPEVTLITA NAEGIEGGKT
TIKSRSVDVG VVESATLADD LTHVEIKARL NSGMEKLLHK DTVFWVVKPQ IGREGISGLG
TLLSGVYIEL QPGAKGSKMD KYDLLDSPPL APPDAKGIRV ILDSKKAGQL SPGDPVLFRG
YRVGSVETST FDTQKRNISY QLFINAPYDR LVTNNVRFWK DSGIAVDLTS AGMRVEMGSL
TTLLSGGVSF DVPEGLDLGQ PVAPKTAFVL YDDQKSIQDS LYTDHIDYLM FFKDSVRGLQ
PGAPVEFRGI RLGTVSKVPF FAPNMRQTFN DDYRIPVLIR IEPERLKMQL GENADVVEHL
GELLKRGLRG SLKTGNLVTG ALYVDLDFYP NTPAITGIRE FNGYQIIPTV SGGLAQIQQR
LMEALDKINK LPLNPMIEQA TSTLSESQRT MKNLQTTLDS MNKILASQSM QQLPTDMQST
LRELNRSMQG FQPGSAAYNK MVADMQRLDQ VLRELQPVLK TLNEKSNALV FEAKDKKDPE
PKRAKQ
