ID   ATG12_CHAGB             Reviewed;         150 AA.
AC   Q2GSG9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Ubiquitin-like protein ATG12;
DE   AltName: Full=Autophagy-related protein 12;
GN   Name=ATG12; ORFNames=CHGG_09085;
OS   Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 /
OS   NRRL 1970) (Soil fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium.
OX   NCBI_TaxID=306901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970;
RX   PubMed=25720678; DOI=10.1128/genomea.00021-15;
RA   Cuomo C.A., Untereiner W.A., Ma L.-J., Grabherr M., Birren B.W.;
RT   "Draft genome sequence of the cellulolytic fungus Chaetomium globosum.";
RL   Genome Announc. 3:E0002115-E0002115(2015).
CC   -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC       transport (Cvt), autophagy vesicles formation, mitophagy, and
CC       nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating
CC       system involving also ATG7 as an E1-like activating enzyme and ATG10 as
CC       an E2-like conjugating enzyme, is essential for its function. The
CC       ATG12-ATG5 conjugate functions as an E3-like enzyme which is required
CC       for lipidation of ATG8 and ATG8 association to the vesicle membranes
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a conjugate with ATG5. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAQ85071.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CH408034; EAQ85071.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001227012.1; XM_001227011.1.
DR   AlphaFoldDB; Q2GSG9; -.
DR   SMR; Q2GSG9; -.
DR   STRING; 38033.XP_001227012.1; -.
DR   EnsemblFungi; EAQ85071; EAQ85071; CHGG_09085.
DR   GeneID; 4395592; -.
DR   eggNOG; KOG3439; Eukaryota.
DR   HOGENOM; CLU_106795_3_1_1; -.
DR   InParanoid; Q2GSG9; -.
DR   OrthoDB; 1525971at2759; -.
DR   Proteomes; UP000001056; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007242; Atg12.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR13385; PTHR13385; 1.
DR   Pfam; PF04110; APG12; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
PE   3: Inferred from homology;
KW   Autophagy; Isopeptide bond; Membrane; Protein transport;
KW   Reference proteome; Transport; Ubl conjugation pathway.
FT   CHAIN           1..150
FT                   /note="Ubiquitin-like protein ATG12"
FT                   /id="PRO_0000317932"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        150
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-128 in ATG5)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   150 AA;  16045 MW;  0DD6ED45865C8F15 CRC64;
     MGGAAQNNNL SNNNNDSPPA SPVLEGRDSP DLPLTMAAST ILTALPRDAT AALAAAGGFP
     QEKVVVRFRP VGSAPAIRRD LVKVISTHKF DSVVAYLRKM LKVPETDGVF LYINNTFAPA
     LDEVVGNLWR CFKDSSDHLI VSYSMSPAFG