PQN41_CAEEL
ID PQN41_CAEEL Reviewed; 2049 AA.
AC C7IVR4; C7IVR5; C7IVR6; G4RT56; G4RT57; G4RT58; S6FMX9;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Polyglutamine-repeat protein pqn-41 {ECO:0000303|PubMed:22363008};
DE AltName: Full=Prion-like-(Q/N-rich)-domain-bearing protein 41 {ECO:0000312|WormBase:F53A3.4b};
GN Name=pqn-41 {ECO:0000312|WormBase:F53A3.4b};
GN ORFNames=F53A3.4 {ECO:0000312|WormBase:F53A3.4b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND D), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DOMAIN, DISRUPTION PHENOTYPE, AND MUTAGENESIS
RP OF GLN-1734; GLN-1737; ALA-1874; GLN-1877; ALA-1998 AND ALA-2001.
RX PubMed=22363008; DOI=10.1126/science.1215156;
RA Blum E.S., Abraham M.C., Yoshimura S., Lu Y., Shaham S.;
RT "Control of nonapoptotic developmental cell death in Caenorhabditis elegans
RT by a polyglutamine-repeat protein.";
RL Science 335:970-973(2012).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: [Isoform d]: In males, required for non-apoptotic death of
CC the linker cell once it has finished guiding gonad elongation at the
CC end of larval development. May be involved in nuclear envelope
CC crenellation in the linker cell. {ECO:0000269|PubMed:22363008}.
CC -!- FUNCTION: [Isoform a]: In males, promotes linker cell survival.
CC {ECO:0000269|PubMed:22363008}.
CC -!- FUNCTION: [Isoform b]: In males, promotes linker cell survival.
CC {ECO:0000269|PubMed:22363008}.
CC -!- SUBCELLULAR LOCATION: [Isoform d]: Cytoplasm
CC {ECO:0000269|PubMed:22363008}. Note=Forms aggregates.
CC {ECO:0000269|PubMed:22363008}.
CC -!- SUBCELLULAR LOCATION: [Isoform a]: Cytoplasm
CC {ECO:0000269|PubMed:22363008}. Note=Does not form aggregates.
CC {ECO:0000269|PubMed:22363008}.
CC -!- SUBCELLULAR LOCATION: [Isoform b]: Cytoplasm
CC {ECO:0000269|PubMed:22363008}. Note=Does not form aggregates.
CC {ECO:0000269|PubMed:22363008}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=b {ECO:0000312|WormBase:F53A3.4b}; Synonyms=PQN-41B
CC {ECO:0000303|PubMed:22363008};
CC IsoId=C7IVR4-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F53A3.4a}; Synonyms=PQN-41A
CC {ECO:0000303|PubMed:22363008};
CC IsoId=C7IVR4-2; Sequence=VSP_057820;
CC Name=c {ECO:0000312|WormBase:F53A3.4c};
CC IsoId=C7IVR4-3; Sequence=VSP_057818, VSP_057819;
CC Name=d {ECO:0000312|WormBase:F53A3.4d}; Synonyms=PQN-41C
CC {ECO:0000303|PubMed:22363008};
CC IsoId=C7IVR4-4; Sequence=VSP_057817;
CC -!- TISSUE SPECIFICITY: Expressed in the linker cell just before it dies.
CC {ECO:0000269|PubMed:22363008}.
CC -!- DOMAIN: The coiled-coil domains in isoform d are important for linker
CC cell death. {ECO:0000269|PubMed:22363008}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes the survival of
CC the linker cell in 16 percent of animals.
CC {ECO:0000269|PubMed:22363008}.
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DR EMBL; BX284603; CCD62158.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD62159.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD62160.1; -; Genomic_DNA.
DR EMBL; BX284603; CDG24084.1; -; Genomic_DNA.
DR RefSeq; NP_001122699.2; NM_001129227.2. [C7IVR4-1]
DR RefSeq; NP_001254849.1; NM_001267920.1. [C7IVR4-3]
DR RefSeq; NP_001293605.1; NM_001306676.1.
DR RefSeq; NP_497478.2; NM_065077.5. [C7IVR4-2]
DR AlphaFoldDB; C7IVR4; -.
DR SMR; C7IVR4; -.
DR IntAct; C7IVR4; 1.
DR STRING; 6239.F53A3.4b; -.
DR EPD; C7IVR4; -.
DR PaxDb; C7IVR4; -.
DR PeptideAtlas; C7IVR4; -.
DR EnsemblMetazoa; F53A3.4a.1; F53A3.4a.1; WBGene00004128. [C7IVR4-2]
DR EnsemblMetazoa; F53A3.4b.1; F53A3.4b.1; WBGene00004128. [C7IVR4-1]
DR EnsemblMetazoa; F53A3.4c.1; F53A3.4c.1; WBGene00004128. [C7IVR4-3]
DR EnsemblMetazoa; F53A3.4d.1; F53A3.4d.1; WBGene00004128. [C7IVR4-4]
DR EnsemblMetazoa; F53A3.4d.2; F53A3.4d.2; WBGene00004128. [C7IVR4-4]
DR EnsemblMetazoa; F53A3.4d.3; F53A3.4d.3; WBGene00004128. [C7IVR4-4]
DR GeneID; 175335; -.
DR KEGG; cel:CELE_F53A3.4; -.
DR CTD; 175335; -.
DR WormBase; F53A3.4a; CE44019; WBGene00004128; pqn-41. [C7IVR4-2]
DR WormBase; F53A3.4b; CE44055; WBGene00004128; pqn-41. [C7IVR4-1]
DR WormBase; F53A3.4c; CE44091; WBGene00004128; pqn-41. [C7IVR4-3]
DR WormBase; F53A3.4d; CE48496; WBGene00004128; pqn-41. [C7IVR4-4]
DR eggNOG; KOG1721; Eukaryota.
DR InParanoid; C7IVR4; -.
DR OrthoDB; 1095647at2759; -.
DR PRO; PR:C7IVR4; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00004128; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Reference proteome.
FT CHAIN 1..2049
FT /note="Polyglutamine-repeat protein pqn-41"
FT /evidence="ECO:0000305"
FT /id="PRO_0000433600"
FT REGION 1..1601
FT /note="Sufficient to prevent linker cell death"
FT /evidence="ECO:0000269|PubMed:22363008"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1134..1198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1236..1265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1481..1616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1636..1661
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1836..1858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 264..302
FT /evidence="ECO:0000255"
FT COILED 396..432
FT /evidence="ECO:0000255"
FT COILED 686..730
FT /evidence="ECO:0000255"
FT COILED 756..796
FT /evidence="ECO:0000255"
FT COILED 822..880
FT /evidence="ECO:0000255"
FT COILED 1659..1685
FT /evidence="ECO:0000255"
FT COILED 1725..1801
FT /evidence="ECO:0000255"
FT COILED 1863..1961
FT /evidence="ECO:0000255"
FT COILED 1991..2041
FT /evidence="ECO:0000255"
FT COMPBIAS 18..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1134..1153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1173..1191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1481..1504
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1517..1536
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1557..1571
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1582..1608
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..1622
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057817"
FT VAR_SEQ 1463..1478
FT /note="RPGRWSGMHVKIATDI -> VVFLKAKIEVFWNFQQ (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_057818"
FT VAR_SEQ 1479..2049
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_057819"
FT VAR_SEQ 1601..1635
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_057820"
FT MUTAGEN 1734
FT /note="Q->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1737; P-1874; P-1877; P-
FT 1998 and P-2001; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
FT MUTAGEN 1737
FT /note="Q->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1734; P-1874; P-1877; P-
FT 1998 and P-2001; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
FT MUTAGEN 1874
FT /note="A->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1734; P-1737; P-1877; P-
FT 1998 and P-2001; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
FT MUTAGEN 1877
FT /note="Q->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1734; P-1737; P-1874; P-
FT 1998 and P-2001; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
FT MUTAGEN 1998
FT /note="A->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1734; P-1737; P-1874; P-
FT 1877 and P-2001; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
FT MUTAGEN 2001
FT /note="A->P: Probable loss of putative coiled-coil
FT structure. Survival of linker cell in 14 percent of
FT animals; when associated with P-1734; P-1737; P-1874; P-
FT 1877 and P-1998; in isoform d."
FT /evidence="ECO:0000269|PubMed:22363008"
SQ SEQUENCE 2049 AA; 229755 MW; 4D065563C1BCA233 CRC64;
MKPKKLQQGS DSAHTSDTES TKTCEKTAKK LPKTQKKLQK SKKTAKKRRD EEFRIFFPPP
RVNPPIKTPF RLHNTNCEHR DWISDNLCLP KYASYQTGWR ISHKSMKSLV LMDKRRAEWL
KMHNLYRKSE IRKAIWTIER KGAKKLDEMP GNWRISREKR NSLDLQEFPI KIEQFPVKKR
EKTAKNWRKI AVLVCFKRKT TTKNRYLRRP KTKNQRKIEF RRPKTSKNRK LRLKIRPRVR
FIDRKLIRRR ECLVDPQNLI SWIRRQEKAE NLRIFQEKQK RLQEQQEDAE WEQIEAQRAN
SDDVIEDKSL KMEVLDIVKH KNRNWIVVEK KGCEIQGMEY LLVLESEFNE QQTVRYDRQN
VDHKFRKMRD EKKEIVEFVS SLPFYKPKPP KINYPTNAGE YEEQEIELER RRLEEEERDQ
NDKKLEIEDR NHFKKWQKRR NLIKIYRNSI RREIWRRRRG RNSTENSDSE SSSEASEPPD
DVITKEEPTD FSEENLVKKE EICDDFEHKI EEDVKPDVYK LNINKMISPP SPPPKKGILL
KKDTKKRGEK RVKTVQFKLT KRQKLAKLWK PPTWQIRQIL RAAADAKGYK IRSGRSRYNE
KIRRLNHFNG QKLGFKSAPT RIDTFEKGID VREQPIPFVE EFVLDDHALL TFASFDDLKA
YEEAYSLRQQ DVIDEFWRHQ CLKNIESFEK DDVERAEMRH EIEKLETEMR CQKMNENAEI
DQENIESFET AGRQIENIIK NTGDCAFETL EEYFSISADF EKNEELRAEE EQLEIEMEHW
ERELEEMIDV IKREFSIENL MMRMLKNRHL LTMRLVVSGT NQSSIDRENL LRKTKKLLEE
LKNLRIAAQN RLKIDFDRNE RMLYRLRNAK QKAAKRARKL VKNWKKSAQN KSGVLKINGN
HVINHDVVVK KWKVELKIEG NGESPRKVVR KAKETSGYWD FRWNFTKFAW KSDVLKRKQR
FGKHSARRAI AFGVKIEEIH SETEFEKLLS EYVEYEESDI QNQVSIDRKI DIITKIKTIT
LNDVRAKAIE MQKQIVEKAV DLMIKSRLDE AAREHQEWLQ SDECKRENQL RQRQQNFFDL
TSSSPATSSF VTTQVVVPRL THLEERLIEL GVEHEVVQHT QRLQSEFENY HHLQQQHNHQ
NFQQQQQGNH DFVTPKAPQD KQKRKYTKRK ALLNTAVASS SDQNGMKSPG SSAMENAAAA
AQAAQAQAQA TIPTPTVNLP DVVAIAAAAA TAQPSAAAAK RPASETPPNG LPKVPRHDEQ
QQQQNNAHSI VMGAREGFLA MNPSLAGHVF PASSASTSGA PGAHSATTSG GAGLIGISAA
TQAQLQAQQA AQAAAAAAAA AAAQATQSLY INTSVAPGAQ AASAQGGGGG QVVAAQQSNQ
AATAEAIRLL QGLPPFLTAG SGSAGIPYFS ALSQQLNQLG AAAPGAPGTL NGLQFPANAA
LGPQLAGAAL LAAVPGAQQQ IKRPGRWSGM HVKIATDIQN YKQSQEKKLP TDIQSTSSSS
AAPASAPAPR AGAGAGATSS SAASSSTSTP SSSSHHKKSS PPHHQKSAAP SAPPRDVTSA
HAPPPPASSA PIVGAPRQGA TPQAAPATTP ATTSQHQQSI QFSQFPPPQL SGGAAYAGNP
QLMAAAINEA TRRVAATPKP PVVRPPSAAT QQQPVSVTSQ ASQQQQQFQQ IQQQRAAAIA
AAAAATSQQA PPAQASQATS AAQQIATSMG LQPAQVTDLV NQHAQQYLLL QQQQQQQQRE
QQQQQQLQAQ QVQQQLIAHL LGGGHQAQQA APAVSVAQQQ QQQVAAAAAA QQQHNAQLQN
IMILTALQQQ MERGAAAGAA ASLPYQLQLA QAQAQAQAQQ APPTSQPSQA ATPQQQQQLD
LIRQMEAVAQ VQQAHAQAQA QAQAQAQQMQ QQQIQQMLMA GQGGPNGQDL IRLLQAAQQQ
SQAQQQQQQQ QAVVAAAQQQ QQQQQHNQQL AAAQAAAAAA AAGRPTQNQY EALLQQQRLL
AAQQQAAAGA SAQQQAAAAA AQAQAQQFQQ QLLGLQPNLL LAQVQQAQQA QAQAQAQAQQ
KPPQMPNGR
