ID   ATG12_DICDI             Reviewed;         124 AA.
AC   Q86CR6; Q54RN5;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Ubiquitin-like protein atg12;
DE   AltName: Full=Autophagy-related protein 12;
DE            Short=APG12-like;
GN   Name=atg12; Synonyms=apg12; ORFNames=DDB_G0282929;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND FUNCTION.
RC   STRAIN=AX3 / DH1;
RX   PubMed=12626495; DOI=10.1074/jbc.m212467200;
RA   Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT   "Macroautophagy is required for multicellular development of the social
RT   amoeba Dictyostelium discoideum.";
RL   J. Biol. Chem. 278:17636-17645(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Ubiquitin-like protein involved in autophagy vesicles
CC       formation. Required for atg8 association to the vesicle membranes.
CC       Conjugated to atg5 through a ubiquitin-like conjugating system
CC       involving also atg7 as an E1-like activating enzyme is essential for
CC       its function (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:12626495}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR   EMBL; AY191017; AAO39080.1; -; Genomic_DNA.
DR   EMBL; AAFI02000049; EAL65908.1; -; Genomic_DNA.
DR   RefSeq; XP_639316.1; XM_634224.1.
DR   AlphaFoldDB; Q86CR6; -.
DR   SMR; Q86CR6; -.
DR   STRING; 44689.DDB0191412; -.
DR   PaxDb; Q86CR6; -.
DR   EnsemblProtists; EAL65908; EAL65908; DDB_G0282929.
DR   GeneID; 8623887; -.
DR   KEGG; ddi:DDB_G0282929; -.
DR   dictyBase; DDB_G0282929; atg12.
DR   eggNOG; KOG3439; Eukaryota.
DR   HOGENOM; CLU_106795_0_1_1; -.
DR   InParanoid; Q86CR6; -.
DR   OMA; LFIYVHQ; -.
DR   PhylomeDB; Q86CR6; -.
DR   Reactome; R-DDI-1632852; Macroautophagy.
DR   Reactome; R-DDI-8934903; Receptor Mediated Mitophagy.
DR   PRO; PR:Q86CR6; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IBA:GO_Central.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR   GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR   GO; GO:0097352; P:autophagosome maturation; IMP:dictyBase.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR   GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR   GO; GO:0006501; P:C-terminal protein lipidation; IBA:GO_Central.
DR   GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
DR   GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IMP:dictyBase.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR   GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR   InterPro; IPR007242; Atg12.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR13385; PTHR13385; 1.
DR   Pfam; PF04110; APG12; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
PE   3: Inferred from homology;
KW   Autophagy; Cytoplasm; Isopeptide bond; Protein transport;
KW   Reference proteome; Transport; Ubl conjugation pathway.
FT   CHAIN           1..124
FT                   /note="Ubiquitin-like protein atg12"
FT                   /id="PRO_0000327635"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..31
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        124
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-187 in atg5)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   124 AA;  14194 MW;  6163660CAD174609 CRC64;
     MEEEEKNNNE VASPTEQNTE STTEQQPQLP VKITSESKII VYFKNAGGAQ PLKQKKFKIQ
     ANVSFQNVID KLRGQLKLKS NESLFLFINQ VFQPSPDEIL GELYKCFSHN DQLIINYSLQ
     MAWG