ID   ATG12_PICGU             Reviewed;         213 AA.
AC   A5DK05;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Ubiquitin-like protein ATG12;
DE   AltName: Full=Autophagy-related protein 12;
GN   Name=ATG12; ORFNames=PGUG_03606;
OS   Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS   / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX   NCBI_TaxID=294746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC       transport (Cvt), autophagy vesicles formation, mitophagy, and
CC       nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating
CC       system involving also ATG7 as an E1-like activating enzyme and ATG10 as
CC       an E2-like conjugating enzyme, is essential for its function. The
CC       ATG12-ATG5 conjugate functions as an E3-like enzyme which is required
CC       for lipidation of ATG8 and ATG8 association to the vesicle membranes
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a conjugate with ATG5. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR   EMBL; CH408158; EDK39509.2; -; Genomic_DNA.
DR   RefSeq; XP_001484226.1; XM_001484176.1.
DR   AlphaFoldDB; A5DK05; -.
DR   SMR; A5DK05; -.
DR   STRING; 4929.XP_001484226.1; -.
DR   EnsemblFungi; EDK39509; EDK39509; PGUG_03606.
DR   GeneID; 5125814; -.
DR   KEGG; pgu:PGUG_03606; -.
DR   VEuPathDB; FungiDB:PGUG_03606; -.
DR   eggNOG; KOG3439; Eukaryota.
DR   HOGENOM; CLU_106795_0_1_1; -.
DR   InParanoid; A5DK05; -.
DR   OMA; RIIHSED; -.
DR   OrthoDB; 1525971at2759; -.
DR   Proteomes; UP000001997; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007242; Atg12.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR13385; PTHR13385; 1.
DR   Pfam; PF04110; APG12; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
PE   3: Inferred from homology;
KW   Autophagy; Isopeptide bond; Membrane; Protein transport;
KW   Reference proteome; Transport; Ubl conjugation pathway.
FT   CHAIN           1..213
FT                   /note="Ubiquitin-like protein ATG12"
FT                   /id="PRO_0000317939"
FT   CROSSLNK        213
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-125 in ATG5)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   213 AA;  23830 MW;  942519D152F1E515 CRC64;
     MTPPTSNSSM VDSLSSWVAE SLSSDMRNNY STSVGKGRAK LACGFYQERW CIDALQKVVS
     INEILCGMSF IHSEPDSDSS TTSADSNTLS QHVDKTSVPL DTSIVVKRLP EVEQRQWHKT
     IKPLPATKIT IRLVPIGSTR AITPKVLQVS SDQTVAVLMR FIAKKLRIST DSVYMYIHNT
     FQPTPDERLG DLYDQFRTNQ ELIFNYCNTV AFG