ID   ATG12_PONAB             Reviewed;         140 AA.
AC   Q5R7W1;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Ubiquitin-like protein ATG12;
DE   AltName: Full=Autophagy-related protein 12;
DE            Short=APG12-like;
GN   Name=ATG12; Synonyms=APG12, APG12L;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Ubiquitin-like protein involved in autophagy vesicles
CC       formation. Conjugation with ATG5 through a ubiquitin-like conjugating
CC       system involving also ATG7 as an E1-like activating enzyme and ATG10 as
CC       an E2-like conjugating enzyme, is essential for its function. The
CC       ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for
CC       lipidation of ATG8 family proteins and their association to the vesicle
CC       membranes. The ATG12-ATG5 conjugate also regulates negatively the
CC       innate antiviral immune response by blocking the type I IFN production
CC       pathway through direct association with RARRES3 and MAVS. Also plays a
CC       role in translation or delivery of incoming viral RNA to the
CC       translation apparatus (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a
CC       complex with several units of ATG16L1. Forms an 800-kDa complex
CC       composed of ATG12-ATG5 and ATG16L2 (By similarity). Interacts with
CC       ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3
CC       and TECPR1 (By similarity). {ECO:0000250|UniProtKB:O94817,
CC       ECO:0000250|UniProtKB:Q9CQY1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Preautophagosomal
CC       structure membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the
CC       autolysosomal membrane. {ECO:0000250}.
CC   -!- DOMAIN: Shares weak sequence similarity with ubiquitin family, but
CC       contains an 'ubiquitin superfold' and the C-terminal Gly is required
CC       for isopeptide linkage. {ECO:0000250}.
CC   -!- PTM: Acetylated by EP300. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR   EMBL; CR859998; CAH92149.1; -; mRNA.
DR   RefSeq; NP_001126258.1; NM_001132786.1.
DR   AlphaFoldDB; Q5R7W1; -.
DR   SMR; Q5R7W1; -.
DR   STRING; 9601.ENSPPYP00000017559; -.
DR   GeneID; 100173230; -.
DR   KEGG; pon:100173230; -.
DR   CTD; 9140; -.
DR   eggNOG; KOG3439; Eukaryota.
DR   InParanoid; Q5R7W1; -.
DR   OrthoDB; 1525971at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR007242; Atg12.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR13385; PTHR13385; 1.
DR   Pfam; PF04110; APG12; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Autophagy; Cytoplasm; Immunity; Innate immunity;
KW   Isopeptide bond; Membrane; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..140
FT                   /note="Ubiquitin-like protein ATG12"
FT                   /id="PRO_0000212473"
FT   REGION          1..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..38
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        140
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-? in acceptor protein)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   140 AA;  15081 MW;  A388985D3B26F253 CRC64;
     MAEEPQTVLQ LPPSSAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK
     AVGDTPIMKT KKWAVERTRT IQGPIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY
     ECFGSDGKLV LHYCKSQAWG