PR19A_ARATH
ID PR19A_ARATH Reviewed; 523 AA.
AC Q94BR4; P93809; Q56Y87;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 155.
DE RecName: Full=Pre-mRNA-processing factor 19 homolog 1 {ECO:0000305};
DE EC=2.3.2.- {ECO:0000250|UniProtKB:O22785};
DE AltName: Full=MOS4-associated complex protein 3A;
DE Short=MAC protein 3A;
DE AltName: Full=Plant U-box protein 59;
DE AltName: Full=U-box domain-containing protein 59;
GN Name=PRP19A; Synonyms=MAC3A, PUB59; OrderedLocusNames=At1g04510;
GN ORFNames=F19P19.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-523.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, COMPONENT OF THE MAC
RP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=19629177; DOI=10.1371/journal.ppat.1000526;
RA Monaghan J., Xu F., Gao M., Zhao Q., Palma K., Long C., Chen S., Zhang Y.,
RA Li X.;
RT "Two Prp19-like U-box proteins in the MOS4-associated complex play
RT redundant roles in plant innate immunity.";
RL PLoS Pathog. 5:E1000526-E1000526(2009).
CC -!- FUNCTION: Probable ubiquitin-protein ligase which is mainly involved
CC pre-mRNA splicing and DNA repair (By similarity). Component of the MAC
CC complex that probably regulates defense responses through
CC transcriptional control and thereby is essential for plant innate
CC immunity. {ECO:0000250|UniProtKB:O22785, ECO:0000269|PubMed:19629177}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:O22785}.
CC -!- SUBUNIT: Homotetramer. Component of the multiprotein assembly MOS4-
CC associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and
CC PRP19 which is related to the PRP19C/Prp19 complex/NTC/Nineteen complex
CC identified in other organisms. Associated with the spliceosome.
CC {ECO:0000250|UniProtKB:P32523, ECO:0000250|UniProtKB:Q9UMS4,
CC ECO:0000269|PubMed:19629177}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19629177}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q94BR4-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB70423.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC000104; AAB70423.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27707.1; -; Genomic_DNA.
DR EMBL; AY039940; AAK64044.1; -; mRNA.
DR EMBL; AY142564; AAN13133.1; -; mRNA.
DR EMBL; AK221436; BAD94465.1; -; mRNA.
DR PIR; E86177; E86177.
DR RefSeq; NP_563708.1; NM_100330.3. [Q94BR4-1]
DR AlphaFoldDB; Q94BR4; -.
DR SMR; Q94BR4; -.
DR BioGRID; 24738; 39.
DR IntAct; Q94BR4; 3.
DR STRING; 3702.AT1G04510.1; -.
DR iPTMnet; Q94BR4; -.
DR PaxDb; Q94BR4; -.
DR PRIDE; Q94BR4; -.
DR ProteomicsDB; 225990; -. [Q94BR4-1]
DR DNASU; 839503; -.
DR EnsemblPlants; AT1G04510.1; AT1G04510.1; AT1G04510. [Q94BR4-1]
DR GeneID; 839503; -.
DR Gramene; AT1G04510.1; AT1G04510.1; AT1G04510. [Q94BR4-1]
DR KEGG; ath:AT1G04510; -.
DR Araport; AT1G04510; -.
DR TAIR; locus:2018319; AT1G04510.
DR eggNOG; KOG0289; Eukaryota.
DR HOGENOM; CLU_023894_1_1_1; -.
DR InParanoid; Q94BR4; -.
DR OMA; HISFELE; -.
DR PhylomeDB; Q94BR4; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q94BR4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94BR4; baseline and differential.
DR Genevisible; Q94BR4; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IGI:TAIR.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PTHR43995; 1.
DR Pfam; PF08606; Prp19; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA damage; DNA repair; Immunity; Innate immunity;
KW mRNA processing; mRNA splicing; Nucleus; Plant defense; Reference proteome;
KW Repeat; Spliceosome; Transferase; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..523
FT /note="Pre-mRNA-processing factor 19 homolog 1"
FT /id="PRO_0000322137"
FT DOMAIN 1..70
FT /note="U-box"
FT REPEAT 220..261
FT /note="WD 1"
FT REPEAT 262..301
FT /note="WD 2"
FT REPEAT 307..346
FT /note="WD 3"
FT REPEAT 352..391
FT /note="WD 4"
FT REPEAT 394..432
FT /note="WD 5"
FT REPEAT 434..470
FT /note="WD 6"
FT REPEAT 479..518
FT /note="WD 7"
FT REGION 136..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 523 AA; 56866 MW; 10593B786D080786 CRC64;
MNCAISGEVP EEPVVSKKSG LLYEKRLIQT HISDYGKCPV TGEPHTLDDI VPIKTGKIVK
PKPLHTASIP GLLGTFQTEW DSLMLSNFAL EQQLHTARQE LSHALYQHDA ACRVIARLKK
ERDESRQLLA EAERQLPAAP EVATSNAALS NGKRGIDDGE QGPNAKKMRL GISAEVITEL
TDCNAALSQQ RKKRQIPKTL ASVDALEKFT QLSSHPLHKT NKPGIFSMDI LHSKDVIATG
GIDTTAVLFD RPSGQILSTL TGHSKKVTSI KFVGDTDLVL TASSDKTVRI WGCSEDGNYT
SRHTLKDHSA EVRAVTVHAT NKYFVSASLD STWCFYDLSS GLCLAQVTDA SENDVNYTAA
AFHPDGLILG TGTAQSIVKI WDVKSQANVA KFGGHNGEIT SISFSENGYF LATAALDGVR
LWDLRKLKNF RTFDFPDANS VEFDHSGSYL GIAASDIRVF QAASVKAEWN PIKTLPDLSG
TGKATSVKFG LDSKYIAVGS MDRNLRIFGL PDDDNTEDSA QDS
