ATG12_VANPO
ID ATG12_VANPO Reviewed; 210 AA.
AC A7TJM4;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Ubiquitin-like protein ATG12;
DE AltName: Full=Autophagy-related protein 12;
GN Name=ATG12; ORFNames=Kpol_534p48;
OS Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX NCBI_TaxID=436907;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC Y-8283 / UCD 57-17;
RX PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT species descended from a whole-genome duplication.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC transport (Cvt), autophagy vesicles formation, mitophagy, and
CC nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating
CC system involving also ATG7 as an E1-like activating enzyme and ATG10 as
CC an E2-like conjugating enzyme, is essential for its function. The
CC ATG12-ATG5 conjugate functions as an E3-like enzyme which is required
CC for lipidation of ATG8 and ATG8 association to the vesicle membranes
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a conjugate with ATG5. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR EMBL; DS480402; EDO17567.1; -; Genomic_DNA.
DR RefSeq; XP_001645425.1; XM_001645375.1.
DR AlphaFoldDB; A7TJM4; -.
DR SMR; A7TJM4; -.
DR STRING; 436907.A7TJM4; -.
DR EnsemblFungi; EDO17567; EDO17567; Kpol_534p48.
DR GeneID; 5545792; -.
DR KEGG; vpo:Kpol_534p48; -.
DR eggNOG; KOG3439; Eukaryota.
DR HOGENOM; CLU_106795_0_0_1; -.
DR InParanoid; A7TJM4; -.
DR OMA; YCYINNS; -.
DR OrthoDB; 1525971at2759; -.
DR PhylomeDB; A7TJM4; -.
DR Proteomes; UP000000267; Unassembled WGS sequence.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR007242; Atg12.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13385; PTHR13385; 1.
DR Pfam; PF04110; APG12; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation pathway.
FT CHAIN 1..210
FT /note="Ubiquitin-like protein ATG12"
FT /id="PRO_0000317942"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 210
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-150 in ATG5)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 210 AA; 23390 MW; D228F24E7FF6100A CRC64;
MSGMLLESES DHEDSEISSG YSENGRLMSQ DPLSLSTGNV MQSRLEQYSK RLSQLGLAAT
DGDSNGDGTD NDIIPSEYDE EEISVPKQEV PMTTSLILDK LPPTTNEFLH QLSIKEMEQD
IEKTVHKIQI KFQPIGSIPL ITPSVCTISS QQTFSMIILF LKKRLKVEQV FCYINNSFAP
NPQQTIGSLW SQFKVNDELI VSYCGTVAFG
