PR1A_TOBAC
ID PR1A_TOBAC Reviewed; 168 AA.
AC P08299;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Pathogenesis-related protein 1A;
DE Short=PR-1A;
DE Flags: Precursor;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Samsun NN;
RX PubMed=3658669; DOI=10.1093/nar/15.17.6799;
RA Cornelissen B.J.C., Horowitz J., van Kan J.A.L., Goldberg R.B., Bol J.F.;
RT "Structure of tobacco genes encoding pathogenesis-related proteins from the
RT PR-1 group.";
RL Nucleic Acids Res. 15:6799-6811(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Samsun NN;
RX PubMed=3691804; DOI=10.1016/0014-5793(87)81166-3;
RA Ohshima M., Matsuoka M., Yamamoto N., Tanaka Y., Kano-Murakami Y.,
RA Ozeki Y., Kato A., Harada N., Ohashi Y.;
RT "Nucleotide sequence of the PR-1 gene of Nicotiana tabacum.";
RL FEBS Lett. 225:243-246(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wisconsin 38;
RA Pfitzner U.M., Pfitzner A.J.P., Goodman H.M.;
RT "DNA sequence analysis of a PR-1a gene from tobacco: molecular relationship
RT of heat shock and pathogen responses in plants.";
RL Mol. Gen. Genet. 211:290-295(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-168.
RC STRAIN=cv. Xanthi;
RX PubMed=3186451; DOI=10.1093/nar/16.20.9861;
RA Cutt J.R., Dixon D.D., Carr J.P., Klessig D.F.;
RT "Isolation and nucleotide sequence of cDNA clones for the pathogenesis-
RT related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc
RT induced by TMV infection.";
RL Nucleic Acids Res. 16:9861-9861(1988).
RN [5]
RP PROTEIN SEQUENCE OF 31-70; 103-131 AND 157-168.
RX PubMed=16453639; DOI=10.1002/j.1460-2075.1985.tb03998.x;
RA Lucas J., Camacho-Henriquez A., Lottspeich F., Henschen A., Sanger H.L.;
RT "Amino acid sequence of the 'pathogenesis-related' leaf protein p14 from
RT viroid-infected tomato reveals a new type of structurally unfamiliar
RT proteins.";
RL EMBO J. 4:2745-2749(1985).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=2026137; DOI=10.1002/j.1460-2075.1991.tb07650.x;
RA Dixon D.C., Cutt J.R., Klessig D.F.;
RT "Differential targeting of the tobacco PR-1 pathogenesis-related proteins
RT to the extracellular space and vacuoles of crystal idioblasts.";
RL EMBO J. 10:1317-1324(1991).
CC -!- FUNCTION: Probably involved in the defense reaction of plants against
CC pathogens.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:2026137}.
CC Note=Accumulates in within the vacuoles of specialized cells known as
CC crystal idioblasts.
CC -!- INDUCTION: Synthesized during pathogen infection or other stress-
CC related responses.
CC -!- PTM: Three disulfide bonds are present. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; X05959; CAA29392.1; -; Genomic_DNA.
DR EMBL; X06361; CAA29660.1; -; Genomic_DNA.
DR EMBL; X12485; CAA31008.1; -; mRNA.
DR EMBL; X06930; CAA30017.1; -; Genomic_DNA.
DR PIR; A05264; A05264.
DR PIR; A24620; A24620.
DR AlphaFoldDB; P08299; -.
DR SMR; P08299; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR002413; V5_allergen-like.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PRINTS; PR00838; V5ALLERGEN.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Pathogenesis-related protein;
KW Plant defense; Reference proteome; Signal; Vacuole.
FT SIGNAL 1..30
FT /evidence="ECO:0000269|PubMed:16453639"
FT CHAIN 31..168
FT /note="Pathogenesis-related protein 1A"
FT /id="PRO_0000006300"
FT DOMAIN 38..156
FT /note="SCP"
FT CONFLICT 5..6
FT /note="LF -> VS (in Ref. 4; CAA31008)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="D -> S (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 68..69
FT /note="SQ -> PS (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="D -> N (in Ref. 3; CAA30017)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="S -> T (in Ref. 3; CAA30017)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="Y -> W (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 168 AA; 18574 MW; 27375217031292E0 CRC64;
MGFVLFSQLP SFLLVSTLLL FLVISHSCRA QNSQQDYLDA HNTARADVGV EPLTWDDQVA
AYAQNYASQL AADCNLVHSH GQYGENLAEG SGDFMTAAKA VEMWVDEKQY YDHDSNTCAQ
GQVCGHYTQV VWRNSVRVGC ARVQCNNGGY VVSCNYDPPG NYRGESPY
