PR2C3_MOUSE
ID PR2C3_MOUSE Reviewed; 224 AA.
AC P04768; P18918; Q498A5; Q6PDB6;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Prolactin-2C3;
DE AltName: Full=Mitogen-regulated protein 2;
DE AltName: Full=Mitogen-regulated protein 3;
DE AltName: Full=Prolactin-2C4;
DE AltName: Full=Proliferin-2;
DE AltName: Full=Proliferin-3;
DE Flags: Precursor;
GN Name=Prl2c3; Synonyms=Mrp2, Mrp3, Mrpplf3, Plf2, Plf3, Prl2c4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND GLYCOSYLATION.
RC STRAIN=BALB/cJ; TISSUE=Placenta;
RX PubMed=3859868; DOI=10.1073/pnas.82.13.4356;
RA Linzer D.I.H., Lee S.-J., Ogren L., Talamantes F., Nathans D.;
RT "Identification of proliferin mRNA and protein in mouse placenta.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:4356-4359(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CD-1; TISSUE=Embryonic fibroblast;
RX PubMed=2790033; DOI=10.1016/0167-4781(89)90081-x;
RA Connor A.M., Waterhouse P., Khokha R., Denhardt D.T.;
RT "Characterization of a mouse mitogen-regulated protein/proliferin gene and
RT its promoter: a member of the growth hormone/prolactin gene superfamily.";
RL Biochim. Biophys. Acta 1009:75-82(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg, and Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 208-224.
RC STRAIN=C57BL/6J;
RX PubMed=8043949; DOI=10.1007/bf00356553;
RA Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y.,
RA Nadeau J.H.;
RT "Genetic mapping of 40 cDNA clones on the mouse genome by PCR.";
RL Mamm. Genome 5:349-355(1994).
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP GLYCOSYLATION.
RX PubMed=10537154; DOI=10.1210/endo.140.11.7142;
RA Fang Y., Lepont P., Fassett J.T., Ford S.P., Mubaidin A., Hamilton R.T.,
RA Nilsen-Hamilton M.;
RT "Signaling between the placenta and the uterus involving the mitogen-
RT regulated protein/proliferins.";
RL Endocrinology 140:5239-5249(1999).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11316781; DOI=10.1210/endo.142.5.8132;
RA Fassett J.T., Nilsen-Hamilton M.;
RT "Mrp3, a mitogen-regulated protein/proliferin gene expressed in wound
RT healing and in hair follicles.";
RL Endocrinology 142:2129-2137(2001).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=16876275; DOI=10.1016/j.neures.2006.05.011;
RA Wang J.W., Jiang Y.N., Huang C.Y., Huang P.Y., Huang M.C., Cheng W.T.,
RA Shen C.K., Ju Y.T.;
RT "Proliferin enhances microvilli formation and cell growth of neuroblastoma
RT cells.";
RL Neurosci. Res. 56:80-90(2006).
CC -!- FUNCTION: May have a role in embryonic development. It is likely to
CC provide a growth stimulus to target cells in maternal and fetal tissues
CC during the development of the embryo at mid-gestation. May play a role
CC during wound healing and in the hair follicle cycle as a growth factor
CC and/or an angiogenesis factor. May play a role in microvilli formation
CC and cell proliferation of neuroblastoma cells.
CC {ECO:0000269|PubMed:11316781, ECO:0000269|PubMed:16876275}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10537154,
CC ECO:0000269|PubMed:16876275}. Endoplasmic reticulum
CC {ECO:0000269|PubMed:16876275}.
CC -!- TISSUE SPECIFICITY: Expressed in placenta and hair follicles, with
CC highest expression levels detected in the outer root sheath and no
CC expression detected in bulb (PubMed:11316781). Expressed in placenta,
CC skin wounds, keratinocytes and weakly in embryonic fibroblasts
CC (PubMed:10537154, PubMed:11316781, PubMed:16876275). Expressed in
CC brain, cerebellum and in Neuro-2a cell line (PubMed:16876275). Not
CC detected in liver, kidney, ovary, pituitary gland and brain
CC (PubMed:3859868). {ECO:0000269|PubMed:10537154,
CC ECO:0000269|PubMed:11316781, ECO:0000269|PubMed:16876275,
CC ECO:0000269|PubMed:3859868}.
CC -!- DEVELOPMENTAL STAGE: Expressed during hair follicle morphogenesis, with
CC highest expression levels detected at late anagen stage of the hair
CC follicle cycle (PubMed:11316781). Expressed in developing brain from
CC embryo to adult (PubMed:16876275). In placenta, detected at 8 dpc,
CC peaks at 10 dpc and declines thereafter (PubMed:10537154).
CC {ECO:0000269|PubMed:10537154, ECO:0000269|PubMed:11316781,
CC ECO:0000269|PubMed:16876275}.
CC -!- PTM: N-glycosylated and sialylated. {ECO:0000269|PubMed:10537154}.
CC -!- SIMILARITY: Belongs to the somatotropin/prolactin family.
CC {ECO:0000305}.
CC -!- CAUTION: Prl2c3 and Prl2c4 have previously been regarded as different
CC proteins, but they seem to be products of the same gene. {ECO:0000305}.
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DR EMBL; K03235; AAA39945.1; -; mRNA.
DR EMBL; X16009; CAA34146.1; -; Genomic_DNA.
DR EMBL; X16010; CAA34146.1; JOINED; Genomic_DNA.
DR EMBL; X16011; CAA34146.1; JOINED; Genomic_DNA.
DR EMBL; X16012; CAA34146.1; JOINED; Genomic_DNA.
DR EMBL; X16013; CAA34146.1; JOINED; Genomic_DNA.
DR EMBL; AK145995; BAE26816.1; -; mRNA.
DR EMBL; AK164068; BAE37612.1; -; mRNA.
DR EMBL; BC058816; AAH58816.1; -; mRNA.
DR EMBL; BC064772; AAH64772.1; -; mRNA.
DR EMBL; BC100299; AAI00300.1; -; mRNA.
DR EMBL; BC132078; AAI32079.1; -; mRNA.
DR EMBL; BC132080; AAI32081.1; -; mRNA.
DR EMBL; U05747; AAB60482.1; -; Genomic_DNA.
DR CCDS; CCDS36595.1; -.
DR PIR; A23159; A23159.
DR PIR; S05648; S05648.
DR RefSeq; NP_035248.2; NM_011118.2.
DR RefSeq; NP_036084.2; NM_011954.2.
DR AlphaFoldDB; P04768; -.
DR SMR; P04768; -.
DR STRING; 10090.ENSMUSP00000097393; -.
DR GlyGen; P04768; 4 sites.
DR PaxDb; P04768; -.
DR PRIDE; P04768; -.
DR ProteomicsDB; 291808; -.
DR DNASU; 18812; -.
DR DNASU; 26421; -.
DR Ensembl; ENSMUST00000099805; ENSMUSP00000097393; ENSMUSG00000056457.
DR GeneID; 18812; -.
DR GeneID; 26421; -.
DR KEGG; mmu:18812; -.
DR KEGG; mmu:26421; -.
DR UCSC; uc007plw.3; mouse.
DR CTD; 18812; -.
DR CTD; 26421; -.
DR MGI; MGI:1341833; Prl2c3.
DR VEuPathDB; HostDB:ENSMUSG00000056457; -.
DR eggNOG; ENOG502QYU3; Eukaryota.
DR GeneTree; ENSGT00950000182818; -.
DR HOGENOM; CLU_088274_0_0_1; -.
DR InParanoid; P04768; -.
DR OMA; MISCHTS; -.
DR OrthoDB; 1290070at2759; -.
DR PhylomeDB; P04768; -.
DR TreeFam; TF332592; -.
DR BioGRID-ORCS; 18812; 9 hits in 40 CRISPR screens.
DR BioGRID-ORCS; 26421; 5 hits in 16 CRISPR screens.
DR ChiTaRS; Prl2c3; mouse.
DR PRO; PR:P04768; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P04768; protein.
DR Bgee; ENSMUSG00000056457; Expressed in placenta and 30 other tissues.
DR ExpressionAtlas; P04768; baseline and differential.
DR Genevisible; P04768; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0008083; F:growth factor activity; IDA:MGI.
DR GO; GO:0005179; F:hormone activity; IDA:MGI.
DR GO; GO:0005148; F:prolactin receptor binding; IBA:GO_Central.
DR GO; GO:0007565; P:female pregnancy; IBA:GO_Central.
DR GO; GO:0071425; P:hematopoietic stem cell proliferation; IDA:MGI.
DR GO; GO:0030879; P:mammary gland development; IBA:GO_Central.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:1902035; P:positive regulation of hematopoietic stem cell proliferation; IDA:MGI.
DR GO; GO:1903489; P:positive regulation of lactation; IBA:GO_Central.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IBA:GO_Central.
DR GO; GO:0031667; P:response to nutrient levels; IBA:GO_Central.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR001400; Somatotropin/Prolactin.
DR InterPro; IPR018116; Somatotropin_CS.
DR PANTHER; PTHR11417; PTHR11417; 1.
DR Pfam; PF00103; Hormone_1; 1.
DR PRINTS; PR00836; SOMATOTROPIN.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00266; SOMATOTROPIN_1; 1.
DR PROSITE; PS00338; SOMATOTROPIN_2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Endoplasmic reticulum; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..224
FT /note="Prolactin-2C3"
FT /id="PRO_0000032968"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 33..40
FT /evidence="ECO:0000250"
FT DISULFID 87..199
FT /evidence="ECO:0000250"
FT DISULFID 216..224
FT /evidence="ECO:0000250"
FT CONFLICT 81
FT /note="D -> G (in Ref. 4; AAH58816)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="L -> S (in Ref. 1; AAA39945)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="D -> N (in Ref. 4; AAH58816)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 224 AA; 25338 MW; C87F3A2310C91320 CRC64;
MLPSSIQPCS WILLLLLVNS SLLWKNVASF PMCAMRNGRC FMSFEDTFEL AGSLSHNISI
EVSELFNEFE KHYSNVSGLR DKSPMRCNTS FLPTPENKEQ ARLTHYAALL KSGAMILDAW
ESPLDDLVSE LSTIKNVPDI IISKATDIKK KINAVRNGVN ALMSTMLQNG DEEKKNPAWF
LQSDNEDARI HSLYGMISCL DNDFKKVDIY LNVLKCYMLK IDNC
