PR38A_MOUSE
ID PR38A_MOUSE Reviewed; 312 AA.
AC Q4FK66; Q6PB58; Q8BVY2;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Pre-mRNA-splicing factor 38A;
GN Name=Prpf38a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J.,
RA Wiemann S., Schick M., Korn B.;
RT "Cloning of mouse full open reading frames in Gateway(R) system entry
RT vector (pDONR201).";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193 AND SER-194, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193; SER-194 AND SER-209, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC spliceosome. {ECO:0000250|UniProtKB:Q8NAV1}.
CC -!- SUBUNIT: Component of the spliceosome B complex. Interacts (via N-
CC terminal interaction domain) with ZMAT2 AND MFAP1.
CC {ECO:0000250|UniProtKB:Q8NAV1}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8NAV1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q4FK66-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4FK66-2; Sequence=VSP_025425, VSP_025426, VSP_025427;
CC -!- SIMILARITY: Belongs to the PRP38 family. {ECO:0000305}.
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DR EMBL; AK075925; BAC36057.1; -; mRNA.
DR EMBL; AK169741; BAE41341.1; -; mRNA.
DR EMBL; CT010186; CAJ18394.1; -; mRNA.
DR EMBL; AL626783; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC059875; AAH59875.1; -; mRNA.
DR CCDS; CCDS18452.1; -. [Q4FK66-1]
DR RefSeq; NP_766285.2; NM_172697.3. [Q4FK66-1]
DR AlphaFoldDB; Q4FK66; -.
DR SMR; Q4FK66; -.
DR IntAct; Q4FK66; 1.
DR STRING; 10090.ENSMUSP00000078207; -.
DR iPTMnet; Q4FK66; -.
DR PhosphoSitePlus; Q4FK66; -.
DR EPD; Q4FK66; -.
DR jPOST; Q4FK66; -.
DR MaxQB; Q4FK66; -.
DR PaxDb; Q4FK66; -.
DR PeptideAtlas; Q4FK66; -.
DR PRIDE; Q4FK66; -.
DR ProteomicsDB; 291545; -. [Q4FK66-1]
DR ProteomicsDB; 291546; -. [Q4FK66-2]
DR Antibodypedia; 53332; 116 antibodies from 23 providers.
DR DNASU; 230596; -.
DR Ensembl; ENSMUST00000079213; ENSMUSP00000078207; ENSMUSG00000063800. [Q4FK66-1]
DR GeneID; 230596; -.
DR KEGG; mmu:230596; -.
DR UCSC; uc008ubf.2; mouse. [Q4FK66-1]
DR CTD; 84950; -.
DR MGI; MGI:1916962; Prpf38a.
DR VEuPathDB; HostDB:ENSMUSG00000063800; -.
DR eggNOG; KOG2889; Eukaryota.
DR GeneTree; ENSGT00730000111085; -.
DR HOGENOM; CLU_039466_1_0_1; -.
DR InParanoid; Q4FK66; -.
DR OMA; FYMRLVG; -.
DR PhylomeDB; Q4FK66; -.
DR TreeFam; TF105910; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 230596; 25 hits in 73 CRISPR screens.
DR ChiTaRS; Prpf38a; mouse.
DR PRO; PR:Q4FK66; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q4FK66; protein.
DR Bgee; ENSMUSG00000063800; Expressed in ear vesicle and 274 other tissues.
DR ExpressionAtlas; Q4FK66; baseline and differential.
DR Genevisible; Q4FK66; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071011; C:precatalytic spliceosome; ISO:MGI.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR InterPro; IPR005037; PRP38.
DR InterPro; IPR024767; PRP38_C.
DR PANTHER; PTHR23142; PTHR23142; 1.
DR Pfam; PF03371; PRP38; 1.
DR Pfam; PF12871; PRP38_assoc; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; mRNA processing; mRNA splicing; Nucleus;
KW Phosphoprotein; Reference proteome; Spliceosome.
FT CHAIN 1..312
FT /note="Pre-mRNA-splicing factor 38A"
FT /id="PRO_0000287274"
FT REGION 1..179
FT /note="N-terminal protein interaction domain"
FT /evidence="ECO:0000250|UniProtKB:Q8NAV1"
FT REGION 181..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 170..204
FT /evidence="ECO:0000255"
FT COMPBIAS 188..204
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..227
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..312
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NAV1"
FT MOD_RES 193
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 194
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 209
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 226
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NAV1"
FT VAR_SEQ 1..141
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025425"
FT VAR_SEQ 204..207
FT /note="LERV -> VRHV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025426"
FT VAR_SEQ 208..312
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025427"
FT CONFLICT 61
FT /note="Y -> F (in Ref. 1; BAC36057)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 37437 MW; 8ED2283514986515 CRC64;
MANRTVKDAH SIHGTNPQYL VEKIIRTRIY ESKYWKEECF GLTAELVVDK AMELKFVGGV
YGGNIKPTPF LCLTLKMLQI QPEKDIIVEF IKNEDFKYVR MLGALYMRLT GTAIDCYKYL
EPLYNDYRKI KSQNRNGEFV LMHVDEFIYE LLHSERVCDI ILPRLQKRYV LEEAEQLEPR
VSALEEDMDD VESSEEEEEE DEKLERVPSP DHRRRSYRDL DKPRRSPALR YRRSRSRSPR
RRSRSPKRRS PSPRRERHRS KSPRRHRSRS RDRRHRSRSK SPGHHRSHRH RSHSKSPERS
KKSHKKSRRG NE
