PR40B_MOUSE
ID PR40B_MOUSE Reviewed; 870 AA.
AC Q80W14; Q5XKB4; Q9CS39; Q9WVC9;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Pre-mRNA-processing factor 40 homolog B;
DE AltName: Full=Huntingtin yeast partner C;
DE AltName: Full=Huntingtin-interacting protein C;
GN Name=Prpf40b; Synonyms=Hypc;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RA Bedford M.T., Das R., Reed R., Leder P.;
RT "FBP11, a mammalian ortholog of the essential yeast splicing factor
RT PRP40.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 777-870 (ISOFORM 1/3).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-851, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the N-terminus of HD. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q80W14-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80W14-2; Sequence=VSP_029122, VSP_029123;
CC Name=3;
CC IsoId=Q80W14-3; Sequence=VSP_029124, VSP_029125;
CC -!- SIMILARITY: Belongs to the PRPF40 family. {ECO:0000305}.
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DR EMBL; AF135440; AAD39464.1; -; mRNA.
DR EMBL; AK019183; BAB31591.1; -; mRNA.
DR EMBL; AK140075; BAE24230.1; -; mRNA.
DR EMBL; BC051961; AAH51961.1; -; mRNA.
DR EMBL; BC082994; AAH82994.1; -; mRNA.
DR CCDS; CCDS27818.1; -. [Q80W14-3]
DR CCDS; CCDS88839.1; -. [Q80W14-1]
DR RefSeq; NP_001335185.1; NM_001348256.1.
DR RefSeq; NP_061256.1; NM_018786.3.
DR AlphaFoldDB; Q80W14; -.
DR SMR; Q80W14; -.
DR STRING; 10090.ENSMUSP00000115869; -.
DR iPTMnet; Q80W14; -.
DR PhosphoSitePlus; Q80W14; -.
DR MaxQB; Q80W14; -.
DR PaxDb; Q80W14; -.
DR PRIDE; Q80W14; -.
DR ProteomicsDB; 289824; -. [Q80W14-1]
DR ProteomicsDB; 289825; -. [Q80W14-2]
DR ProteomicsDB; 289826; -. [Q80W14-3]
DR DNASU; 54614; -.
DR GeneID; 54614; -.
DR KEGG; mmu:54614; -.
DR UCSC; uc007xph.1; mouse. [Q80W14-3]
DR CTD; 25766; -.
DR MGI; MGI:1925583; Prpf40b.
DR eggNOG; KOG0152; Eukaryota.
DR InParanoid; Q80W14; -.
DR OrthoDB; 1112854at2759; -.
DR TreeFam; TF318732; -.
DR BioGRID-ORCS; 54614; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Prpf40b; mouse.
DR PRO; PR:Q80W14; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q80W14; protein.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005685; C:U1 snRNP; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd00201; WW; 2.
DR Gene3D; 1.10.10.440; -; 5.
DR InterPro; IPR002713; FF_domain.
DR InterPro; IPR036517; FF_domain_sf.
DR InterPro; IPR039726; Prp40-like.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11864; PTHR11864; 1.
DR Pfam; PF01846; FF; 2.
DR Pfam; PF00397; WW; 2.
DR SMART; SM00441; FF; 4.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF51045; SSF51045; 2.
DR SUPFAM; SSF81698; SSF81698; 5.
DR PROSITE; PS51676; FF; 6.
DR PROSITE; PS01159; WW_DOMAIN_1; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Isopeptide bond;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Ubl conjugation.
FT CHAIN 1..870
FT /note="Pre-mRNA-processing factor 40 homolog B"
FT /id="PRO_0000309283"
FT DOMAIN 92..125
FT /note="WW 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT DOMAIN 133..166
FT /note="WW 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT DOMAIN 276..330
FT /note="FF 1"
FT DOMAIN 340..397
FT /note="FF 2"
FT DOMAIN 410..470
FT /note="FF 3"
FT DOMAIN 490..550
FT /note="FF 4"
FT DOMAIN 554..610
FT /note="FF 5"
FT DOMAIN 625..682
FT /note="FF 6"
FT REGION 146..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 690..870
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 604..640
FT /evidence="ECO:0000255"
FT COMPBIAS 161..175
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..215
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..277
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 693..711
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 712..740
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 778..792
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 793..826
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 840..870
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 148
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6NWY9"
FT MOD_RES 764
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6NWY9"
FT MOD_RES 831
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6NWY9"
FT MOD_RES 851
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 175
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q6NWY9"
FT CROSSLNK 837
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q6NWY9"
FT VAR_SEQ 538..557
FT /note="STPLDLFKFYVEELKARFHD -> KAARLPLVCSAFLPCQPLWT (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029122"
FT VAR_SEQ 558..870
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029123"
FT VAR_SEQ 685
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029124"
FT VAR_SEQ 714
FT /note="S -> SVSRQ (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029125"
FT CONFLICT 616
FT /note="T -> R (in Ref. 1; AAD39464)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 870 AA; 99300 MW; 4D0C2429AF35EAD7 CRC64;
MMPPPFMPPP GLPPPFPPMG LPPMSQRPPA IPPMPPGILP PMLPPMGAPP PLTQIPGMVP
PMMPGMLMPA VPVTAATAPG ADTASSAVAG TGPPRALWSE HVAPDGRIYY YNADDKQSVW
EKPSVLKSKA ELLLSQCPWK EYKSDTGKPY YYNNQSQESR WTRPKDLDDL EALVKQESAG
KQQTQQLQTL QPQPPQPQPD PPPIPPGPIP VPMALLEPEP GRSEDCDVLE AAQPLEQGFL
QREEGPSSST GQHRQPQEEE EAKPEPERSG LSWSNREKAK QAFKELLRDK AVPSNASWEQ
AMKMVVTDPR YSALPKLSEK KQAFNAYKAQ REKEEKEEAR LRAKEAKQTL QHFLEQHERM
TSTTRYRRAE QTFGDLEVWA VVPERERKEV YDDVLFFLAK KEKEQAKQLR RRNIQALKSI
LDGMSSVNFQ TTWSQAQQYL MDNPSFAQDQ QLQNMDKEDA LICFEEHIRA LEREEEEERE
RARLRERRQQ RKNREAFQSF LDELHETGQL HSMSTWMELY PAVSTDVRFA NMLGQPGSTP
LDLFKFYVEE LKARFHDEKK IIKDILKDRG FCVEVNTAFE DFAHVISFDK RAAALDAGNI
KLTFNSLLEK AEARETEREK EEARRMRRRE AAFRSMLRQA VPALELGTAW EEVRERFVCD
SAFEQITLES ERIRLFREFL QVLEQTECQH LHTKGRKHGR KGKKHHRKRS HSPSGSESDE
EELPPPSLRP PKRRRRNPSE SGSEPSSSLD SVESGGAALG GPGSPSSHLL LGSDHGLRKT
KKPKKKTKKR RHKSTSPDSE TDPEDKAGKE SEDREQEQDR EPRQAELPNR SPGFGIKKEK
TGWDTSESEL SEGELERRRR TLLQQLDDHQ
