ATG13_CAEEL
ID ATG13_CAEEL Reviewed; 443 AA.
AC P34379;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Autophagy-related protein 13 homolog;
GN Name=atg-13 {ECO:0000303|PubMed:19377305, ECO:0000312|WormBase:D2007.5};
GN Synonyms=epg-1 {ECO:0000303|PubMed:19377305, ECO:0000312|WormBase:D2007.5};
GN ORFNames=D2007.5 {ECO:0000312|WormBase:D2007.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH UNC-51, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19377305; DOI=10.4161/auto.5.5.8624;
RA Tian E., Wang F., Han J., Zhang H.;
RT "epg-1 functions in autophagy-regulated processes and may encode a highly
RT divergent Atg13 homolog in C. elegans.";
RL Autophagy 5:608-615(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH LGG-1.
RX PubMed=26687600; DOI=10.1016/j.molcel.2015.11.019;
RA Wu F., Watanabe Y., Guo X.Y., Qi X., Wang P., Zhao H.Y., Wang Z.,
RA Fujioka Y., Zhang H., Ren J.Q., Fang T.C., Shen Y.X., Feng W., Hu J.J.,
RA Noda N.N., Zhang H.;
RT "Structural Basis of the Differential Function of the Two C. elegans Atg8
RT Homologs, LGG-1 and LGG-2, in Autophagy.";
RL Mol. Cell 60:914-929(2015).
CC -!- FUNCTION: Component of the unc-51/atg-13 complex required for
CC autophagosome formation (PubMed:19377305, PubMed:26687600). Required
CC for the degradation of germ cell specific P-granule components such as
CC sepa-1 by autophagy in somatic cells (PubMed:19377305). This ensures
CC exclusive localization of the P-granules in germ cells
CC (PubMed:19377305). May function downstream of the let-363 (Tor)
CC signaling pathway to mediate sepa-1 degradation (PubMed:19377305).
CC Plays a role in survival during limited food availability
CC (PubMed:19377305). {ECO:0000269|PubMed:19377305,
CC ECO:0000269|PubMed:26687600}.
CC -!- SUBUNIT: Interacts with unc-51 (via C-terminus) (PubMed:19377305).
CC Interacts with lgg-1; the interaction is direct (PubMed:26687600).
CC {ECO:0000269|PubMed:19377305, ECO:0000269|PubMed:26687600}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:O75143}. Cytoplasm
CC {ECO:0000269|PubMed:19377305}. Preautophagosomal structure
CC {ECO:0000269|PubMed:26687600}. Perikaryon
CC {ECO:0000269|PubMed:19377305}. Cell projection, axon
CC {ECO:0000269|PubMed:19377305}. Note=Under starvation conditions, is
CC localized to punctate structures primarily representing the isolation
CC membrane that sequesters a portion of the cytoplasm resulting in the
CC formation of an autophagosome. Recruited to preautophagosomes by lgg-1
CC (PubMed:26687600). {ECO:0000250|UniProtKB:O75143,
CC ECO:0000269|PubMed:26687600}.
CC -!- DEVELOPMENTAL STAGE: Expressed from the 8-cell stage and subsequently
CC throughout embryogenesis. After hatching, highly expressed in neurons
CC including nerve ring cells, neurons in the tail and DD/VD motor neurons
CC in the ventral nerve cord. Also expressed in body wall muscle and
CC pharyngeal muscle. {ECO:0000269|PubMed:19377305}.
CC -!- SIMILARITY: Belongs to the ATG13 family. Metazoan subfamily.
CC {ECO:0000305}.
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DR EMBL; FO080532; CCD64450.1; -; Genomic_DNA.
DR PIR; S44786; S44786.
DR RefSeq; NP_498782.1; NM_066381.4.
DR AlphaFoldDB; P34379; -.
DR SMR; P34379; -.
DR BioGRID; 41356; 5.
DR ComplexPortal; CPX-4821; unc-51-atg-13 complex.
DR STRING; 6239.D2007.5.1; -.
DR EPD; P34379; -.
DR PaxDb; P34379; -.
DR PeptideAtlas; P34379; -.
DR EnsemblMetazoa; D2007.5.1; D2007.5.1; WBGene00017045.
DR GeneID; 176150; -.
DR KEGG; cel:CELE_D2007.5; -.
DR CTD; 176150; -.
DR WormBase; D2007.5; CE00130; WBGene00017045; atg-13.
DR eggNOG; KOG3874; Eukaryota.
DR GeneTree; ENSGT00390000007055; -.
DR HOGENOM; CLU_050282_0_0_1; -.
DR InParanoid; P34379; -.
DR OMA; MHFEEPE; -.
DR OrthoDB; 935872at2759; -.
DR Reactome; R-CEL-1632852; Macroautophagy.
DR PRO; PR:P34379; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00017045; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:1990316; C:Atg1/ULK1 kinase complex; IBA:GO_Central.
DR GO; GO:0005776; C:autophagosome; IC:ComplexPortal.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR GO; GO:0000407; C:phagophore assembly site; IBA:GO_Central.
DR GO; GO:1902554; C:serine/threonine protein kinase complex; IC:ComplexPortal.
DR GO; GO:0000045; P:autophagosome assembly; IC:ComplexPortal.
DR GO; GO:0006914; P:autophagy; IC:ComplexPortal.
DR GO; GO:0016236; P:macroautophagy; IC:ComplexPortal.
DR GO; GO:0000423; P:mitophagy; IBA:GO_Central.
DR GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IBA:GO_Central.
DR GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR Gene3D; 3.30.900.10; -; 1.
DR InterPro; IPR040182; ATG13.
DR InterPro; IPR036570; HORMA_dom_sf.
DR PANTHER; PTHR13430; PTHR13430; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cell projection; Cytoplasm; Reference proteome.
FT CHAIN 1..443
FT /note="Autophagy-related protein 13 homolog"
FT /id="PRO_0000065267"
FT REGION 232..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 308..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..272
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 443 AA; 49800 MW; 15B085155B8B485B CRC64;
MVNEYDTYNK WLKFFSVRMV QSIIQSRLGD EIESKCVPYS ENAVDWFNMR IDELGEISAY
LKSNIKSYPP VGTLTLEFLL YTPSGQLLPL EAWILSSSEG TDDCSRNELY HDMSTLLRSA
IVSARMTPMH RLYVKKQHLE TFVIMYRVFE NDISSDMGKG KKTRKIGELV SKFGNISLDL
HYRTSMHFEE PEIAPVTPVE DVEEEIDDGE KTVVDENIQT RTVSECVPIA DAKKRKASGS
VESATSAGSS TSREAAPRFI LGQSTSSEDS RHSDVQNSYE EDHKPSLADL RNHSFPFVNL
LQSAYNPANG TKKNSSSTCL NSPKSTPEDK EPTIEKVAES FRAAKIDEVV FEEDEDEELP
LDSMELSEDS FVHFNQLSDF GGAPSLGNEL GDYLKQLKTA PDMTESGDID ICNMDLKTEL
EKISSQTANF NNFLKHVNSF SDE
