PRA1_YEAST
ID PRA1_YEAST Reviewed; 176 AA.
AC P53633; D6W1D5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Prenylated Rab acceptor 1;
GN Name=YIP3; Synonyms=PRA1; OrderedLocusNames=YNL044W; ORFNames=N2650;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Matern H.T., Gallwitz D.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8740423;
RX DOI=10.1002/(sici)1097-0061(199604)12:5<493::aid-yea929>3.0.co;2-w;
RA Nasr F., Becam A.-M., Herbert C.J.;
RT "The sequence of 12.8 kb from the left arm of chromosome XIV reveals a
RT sigma element, a pro-tRNA and six complete open reading frames, one of
RT which encodes a protein similar to the human leukotriene A4 hydrolase.";
RL Yeast 12:493-499(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP INTERACTION WITH SEC4; YIP1; YPT1; YPT6; YPT10; YPT11; YPT31; YPT32 AND
RP YPT52.
RX PubMed=11785952; DOI=10.1006/bbrc.2001.6242;
RA Calero M., Collins R.N.;
RT "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab
RT proteins.";
RL Biochem. Biophys. Res. Commun. 290:676-681(2002).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- SUBUNIT: Interacts with YIP1 and the Rab GTPases SEC4, YPT1, YPT6,
CC YPT10, YPT11, YPT31, YPT32 and YPT52. {ECO:0000269|PubMed:11785952}.
CC -!- INTERACTION:
CC P53633; P07560: SEC4; NbExp=2; IntAct=EBI-25301, EBI-16858;
CC P53633; P01123: YPT1; NbExp=2; IntAct=EBI-25301, EBI-29496;
CC P53633; P38146: YPT10; NbExp=2; IntAct=EBI-25301, EBI-29357;
CC P53633; P48559: YPT11; NbExp=2; IntAct=EBI-25301, EBI-29362;
CC P53633; P38555: YPT31; NbExp=3; IntAct=EBI-25301, EBI-29379;
CC P53633; P51996: YPT32; NbExp=2; IntAct=EBI-25301, EBI-29384;
CC P53633; P36018: YPT52; NbExp=2; IntAct=EBI-25301, EBI-29407;
CC P53633; Q99260: YPT6; NbExp=2; IntAct=EBI-25301, EBI-29503;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 5500 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PRA1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA64238.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X97400; CAA66057.1; -; Genomic_DNA.
DR EMBL; X97401; CAA66058.1; -; mRNA.
DR EMBL; X94547; CAA64238.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z71320; CAA95911.1; -; Genomic_DNA.
DR EMBL; Z71321; CAA95913.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10501.1; -; Genomic_DNA.
DR PIR; S61100; S61100.
DR RefSeq; NP_014354.2; NM_001182883.1.
DR AlphaFoldDB; P53633; -.
DR SMR; P53633; -.
DR BioGRID; 35780; 128.
DR DIP; DIP-2059N; -.
DR IntAct; P53633; 31.
DR MINT; P53633; -.
DR STRING; 4932.YNL044W; -.
DR TCDB; 9.A.49.1.12; the prenylated rab acceptor protein 1 (pra1) family.
DR iPTMnet; P53633; -.
DR MaxQB; P53633; -.
DR PaxDb; P53633; -.
DR PRIDE; P53633; -.
DR TopDownProteomics; P53633; -.
DR EnsemblFungi; YNL044W_mRNA; YNL044W; YNL044W.
DR GeneID; 855683; -.
DR KEGG; sce:YNL044W; -.
DR SGD; S000004989; YIP3.
DR VEuPathDB; FungiDB:YNL044W; -.
DR eggNOG; KOG3142; Eukaryota.
DR GeneTree; ENSGT00390000010549; -.
DR HOGENOM; CLU_103851_1_1_1; -.
DR InParanoid; P53633; -.
DR OMA; AAFGCHK; -.
DR BioCyc; YEAST:G3O-33079-MON; -.
DR ChiTaRS; YIP3; yeast.
DR PRO; PR:P53633; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53633; protein.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IPI:SGD.
DR InterPro; IPR004895; Prenylated_rab_accept_PRA1.
DR PANTHER; PTHR19317; PTHR19317; 1.
DR Pfam; PF03208; PRA1; 1.
PE 1: Evidence at protein level;
KW Acetylation; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..176
FT /note="Prenylated Rab acceptor 1"
FT /id="PRO_0000220887"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 29
FT /note="L -> F (in Ref. 1; CAA66057/CAA66058)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 176 AA; 19412 MW; C88401CB23197CD8 CRC64;
MNQLGALAQV SRFTQNFSME NIKSEFQSLQ SKLATLRTPQ EFFNFKKISK PQNFGEVQSR
VAYNLKYFSS NYGLIIGCLS IYTLLTNLLL LFVIVLVVAG IVGINKLKGE ELVTPFGSFK
TNQLYTGLVC VAVPIGFLAS PISTLLWLIG ASAVSVFGHA SLMEKPIETV FDEETV
