ATG13_DROME
ID ATG13_DROME Reviewed; 523 AA.
AC Q9VHR6;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Autophagy-related protein 13 homolog;
GN Name=Atg13; ORFNames=CG7331;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; THR-238; SER-314 AND
RP SER-317, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP FUNCTION, PHOSPHORYLATION, AND INTERACTION WITH ATG1.
RX PubMed=19225150; DOI=10.1091/mbc.e08-12-1250;
RA Chang Y.Y., Neufeld T.P.;
RT "An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy
RT regulation.";
RL Mol. Biol. Cell 20:2004-2014(2009).
RN [6]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=20713604; DOI=10.1083/jcb.201002035;
RA Nezis I.P., Shravage B.V., Sagona A.P., Lamark T., Bjorkoy G., Johansen T.,
RA Rusten T.E., Brech A., Baehrecke E.H., Stenmark H.;
RT "Autophagic degradation of dBruce controls DNA fragmentation in nurse cells
RT during late Drosophila melanogaster oogenesis.";
RL J. Cell Biol. 190:523-531(2010).
CC -!- FUNCTION: Autophagy factor required for autophagosome formation. Target
CC of the TOR kinase signaling pathway that regulates autophagy through
CC the control of the phosphorylation status of Atg13 and Atg1. The Atg1-
CC Atg13 complex functions at multiple levels to mediate and adjust
CC nutrient-dependent autophagic signaling. Involved in the autophagic
CC degradation of dBruce which controls DNA fragmentation in nurse cells.
CC {ECO:0000269|PubMed:19225150, ECO:0000269|PubMed:20713604}.
CC -!- SUBUNIT: Interacts with Atg1. {ECO:0000269|PubMed:19225150}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC Preautophagosomal structure {ECO:0000250}. Note=Under starvation
CC conditions, is localized to puncate structures primarily representing
CC the isolation membrane that sequesters a portion of the cytoplasm
CC resulting in the formation of an autophagosome. {ECO:0000250}.
CC -!- PTM: Phosphorylated in a nutrient-, TOR- and Atg1 kinase-dependent
CC manner. {ECO:0000269|PubMed:18327897, ECO:0000269|PubMed:19225150}.
CC -!- DISRUPTION PHENOTYPE: Results in late stage egg chambers that contain
CC persisting nurse cell nuclei without fragmented DNA and attenuation of
CC caspase-3 cleavage. {ECO:0000269|PubMed:20713604}.
CC -!- SIMILARITY: Belongs to the ATG13 family. Metazoan subfamily.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF54234.1; -; Genomic_DNA.
DR EMBL; AY061109; AAL28657.1; -; mRNA.
DR RefSeq; NP_649796.1; NM_141539.4.
DR AlphaFoldDB; Q9VHR6; -.
DR SMR; Q9VHR6; -.
DR BioGRID; 66179; 7.
DR IntAct; Q9VHR6; 2.
DR STRING; 7227.FBpp0081333; -.
DR iPTMnet; Q9VHR6; -.
DR PaxDb; Q9VHR6; -.
DR PRIDE; Q9VHR6; -.
DR DNASU; 40998; -.
DR EnsemblMetazoa; FBtr0081843; FBpp0081333; FBgn0261108.
DR GeneID; 40998; -.
DR KEGG; dme:Dmel_CG7331; -.
DR UCSC; CG7331-RA; d. melanogaster.
DR CTD; 9776; -.
DR FlyBase; FBgn0261108; Atg13.
DR VEuPathDB; VectorBase:FBgn0261108; -.
DR eggNOG; KOG3874; Eukaryota.
DR GeneTree; ENSGT00390000007055; -.
DR HOGENOM; CLU_036365_1_1_1; -.
DR InParanoid; Q9VHR6; -.
DR OMA; MSAQRLN; -.
DR OrthoDB; 926357at2759; -.
DR PhylomeDB; Q9VHR6; -.
DR Reactome; R-DME-1632852; Macroautophagy.
DR BioGRID-ORCS; 40998; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40998; -.
DR PRO; PR:Q9VHR6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0261108; Expressed in capitellum (Drosophila) and 28 other tissues.
DR Genevisible; Q9VHR6; DM.
DR GO; GO:1990316; C:Atg1/ULK1 kinase complex; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0000407; C:phagophore assembly site; IBA:GO_Central.
DR GO; GO:0019887; F:protein kinase regulator activity; ISS:FlyBase.
DR GO; GO:0032147; P:activation of protein kinase activity; ISS:FlyBase.
DR GO; GO:0006914; P:autophagy; IMP:FlyBase.
DR GO; GO:0060729; P:intestinal epithelial structure maintenance; IMP:FlyBase.
DR GO; GO:0035096; P:larval midgut cell programmed cell death; IMP:FlyBase.
DR GO; GO:0000423; P:mitophagy; IBA:GO_Central.
DR GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IBA:GO_Central.
DR GO; GO:0045850; P:positive regulation of nurse cell apoptotic process; IMP:FlyBase.
DR GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR GO; GO:0010506; P:regulation of autophagy; IMP:FlyBase.
DR Gene3D; 3.30.900.10; -; 1.
DR InterPro; IPR040182; ATG13.
DR InterPro; IPR018731; Atg13_N.
DR InterPro; IPR036570; HORMA_dom_sf.
DR PANTHER; PTHR13430; PTHR13430; 2.
DR Pfam; PF10033; ATG13; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..523
FT /note="Autophagy-related protein 13 homolog"
FT /id="PRO_0000345156"
FT REGION 504..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 227
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 238
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 314
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 523 AA; 56927 MW; 97EE8BAD91CD0004 CRC64;
MSAQRLNAAE RDLEKFIKFL VLKSTQVVVQ SRLGEKMQTQ CNPLAGSDWF NIAVQDHPEV
LDETKRALNL KTGESILQRL PLCVEISLKT TEGDQMVLEV WSLDLLQPQN GASPATNDLN
PEGQTLKAAH AIYNRMGIML KSLISLTRTT PAYKLSRRQC PDSYGIFYRI YVDRPQVHTL
GEGHKHVKIG QLSTIVGSLV MSVAYRTKLT ISPTAAQSES NTIMLKSDHF RPATDANTPG
NQQQTQNGTV VAKKLGLGAL NPAQGTADRR FIDIEKPLRP GAFTDMGKLK QYTEDDFVLP
ETPPFEWLLR GRGSVESLNR LDNNSVASVN ISNNNNSTQD SKFNQISNLN NNSAGFKSFE
KNSENSVSPI KSLLIPASAT ATYRHHSEPS LQPPPDDDNL LKELHFPFAS PTSHVNDLAK
FYRECYHAPP LKGLNELQAE ISSISSTPPA SSGSGGVAAC GPTAAATAIA TSSADASAMD
DLSRQLEQFE TSLEDYDKLV SQFGLTGSSS TGSRSSGGLQ MSN
