PRAF2_HUMAN
ID PRAF2_HUMAN Reviewed; 178 AA.
AC O60831; B2RD20;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=PRA1 family protein 2;
GN Name=PRAF2; ORFNames=JM4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Strom T.M., Nyakatura G., Hellebrand H., Drescher B., Rosenthal A.,
RA Meindl A.;
RT "Transcription map in Xp11.23.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=B-cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH CCR5.
RX PubMed=15757671; DOI=10.1016/j.febslet.2005.02.037;
RA Schweneker M., Bachmann A.S., Moelling K.;
RT "JM4 is a four-transmembrane protein binding to the CCR5 receptor.";
RL FEBS Lett. 579:1751-1758(2005).
RN [7]
RP GENOMIC ORGANIZATION, AND TISSUE SPECIFICITY.
RX PubMed=16481131; DOI=10.1016/j.gene.2005.12.009;
RA Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.;
RT "Genomic organization, expression profile, and characterization of the new
RT protein PRA1 domain family, member 2 (PRAF2).";
RL Gene 371:154-165(2006).
RN [8]
RP INTERACTION WITH GDE1.
RX PubMed=16472945; DOI=10.1016/j.gene.2005.11.023;
RA Bachmann A.S., Duennebier F.F., Mocz G.;
RT "Genomic organization, characterization, and molecular 3D model of GDE1, a
RT novel mammalian glycerophosphoinositol phosphodiesterase.";
RL Gene 371:144-153(2006).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17975142; DOI=10.1158/1078-0432.ccr-07-0829;
RA Geerts D., Wallick C.J., Koomoa D.-L., Koster J., Versteeg R., Go R.C.V.,
RA Bachmann A.S.;
RT "Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in
RT neuroblastoma: correlation with clinical features, cellular localization,
RT and cerulenin-mediated apoptosis regulation.";
RL Clin. Cancer Res. 13:6312-6319(2007).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=18395978; DOI=10.1016/j.neulet.2008.03.030;
RA Koomoa D.-L., Go R.C.V., Wester K., Bachmann A.S.;
RT "Expression profile of PRAF2 in the human brain and enrichment in synaptic
RT vesicles.";
RL Neurosci. Lett. 436:171-176(2008).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: May be involved in ER/Golgi transport and vesicular traffic.
CC Plays a proapoptotic role in cerulenin-induced neuroblastoma apoptosis.
CC {ECO:0000269|PubMed:17975142, ECO:0000269|PubMed:18395978}.
CC -!- SUBUNIT: Interacts with CCR5 and GDE1. {ECO:0000269|PubMed:15757671,
CC ECO:0000269|PubMed:16472945}.
CC -!- INTERACTION:
CC O60831; O95870: ABHD16A; NbExp=3; IntAct=EBI-2506064, EBI-348517;
CC O60831; Q6QNK2: ADGRD1; NbExp=3; IntAct=EBI-2506064, EBI-18053335;
CC O60831; Q8TD06: AGR3; NbExp=3; IntAct=EBI-2506064, EBI-3925742;
CC O60831; Q13520: AQP6; NbExp=3; IntAct=EBI-2506064, EBI-13059134;
CC O60831; P11912: CD79A; NbExp=3; IntAct=EBI-2506064, EBI-7797864;
CC O60831; Q9H5X1: CIAO2A; NbExp=3; IntAct=EBI-2506064, EBI-752069;
CC O60831; Q8IUN9: CLEC10A; NbExp=3; IntAct=EBI-2506064, EBI-2873246;
CC O60831; O00559: EBAG9; NbExp=3; IntAct=EBI-2506064, EBI-8787095;
CC O60831; P36382: GJA5; NbExp=3; IntAct=EBI-2506064, EBI-750433;
CC O60831; P48165: GJA8; NbExp=3; IntAct=EBI-2506064, EBI-17458373;
CC O60831; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-2506064, EBI-13345167;
CC O60831; P31937: HIBADH; NbExp=3; IntAct=EBI-2506064, EBI-11427100;
CC O60831; Q8NBQ5: HSD17B11; NbExp=3; IntAct=EBI-2506064, EBI-1052304;
CC O60831; Q8IX19: MCEMP1; NbExp=3; IntAct=EBI-2506064, EBI-2816356;
CC O60831; Q9NQG6: MIEF1; NbExp=3; IntAct=EBI-2506064, EBI-740987;
CC O60831; Q9ULP0-2: NDRG4; NbExp=3; IntAct=EBI-2506064, EBI-11978907;
CC O60831; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-2506064, EBI-7545592;
CC O60831; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-2506064, EBI-10192441;
CC O60831; Q99942: RNF5; NbExp=3; IntAct=EBI-2506064, EBI-348482;
CC O60831; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-2506064, EBI-18159983;
CC O60831; Q96MV1: TLCD4; NbExp=3; IntAct=EBI-2506064, EBI-12947623;
CC O60831; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-2506064, EBI-8638294;
CC O60831; Q9Y320: TMX2; NbExp=3; IntAct=EBI-2506064, EBI-6447886;
CC O60831; Q96MV8: ZDHHC15; NbExp=3; IntAct=EBI-2506064, EBI-12837904;
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000305|PubMed:17975142};
CC Multi-pass membrane protein {ECO:0000305|PubMed:17975142}.
CC -!- TISSUE SPECIFICITY: Strong expression in the brain, small intestine,
CC lung, spleen, and pancreas as well as in tumor tissues of the breast,
CC colon, lung and ovary, with a weaker expression in normal tissues of
CC the same patient. High expression in neuroblastic tumors. Strongly
CC expressed in Purkinje cells and more moderately in cells of the
CC molecular and the granular layers in the cerebellum. Detected in
CC neuronal cells, but not in non-neuronal cells in the cerebral cortex,
CC hippocampus, and lateral ventricles. {ECO:0000269|PubMed:16481131,
CC ECO:0000269|PubMed:17975142, ECO:0000269|PubMed:18395978}.
CC -!- SIMILARITY: Belongs to the PRA1 family. {ECO:0000305}.
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DR EMBL; AJ005896; CAA06753.1; -; mRNA.
DR EMBL; CR457023; CAG33304.1; -; mRNA.
DR EMBL; AK315374; BAG37767.1; -; mRNA.
DR EMBL; CH471224; EAW50706.1; -; Genomic_DNA.
DR EMBL; BC021213; AAH21213.1; -; mRNA.
DR CCDS; CCDS14317.1; -.
DR RefSeq; NP_009144.1; NM_007213.2.
DR AlphaFoldDB; O60831; -.
DR BioGRID; 116396; 97.
DR IntAct; O60831; 66.
DR MINT; O60831; -.
DR STRING; 9606.ENSP00000451962; -.
DR iPTMnet; O60831; -.
DR PhosphoSitePlus; O60831; -.
DR SwissPalm; O60831; -.
DR BioMuta; PRAF2; -.
DR EPD; O60831; -.
DR jPOST; O60831; -.
DR MassIVE; O60831; -.
DR MaxQB; O60831; -.
DR PaxDb; O60831; -.
DR PeptideAtlas; O60831; -.
DR PRIDE; O60831; -.
DR ProteomicsDB; 49623; -.
DR Antibodypedia; 34944; 152 antibodies from 29 providers.
DR DNASU; 11230; -.
DR Ensembl; ENST00000553851.3; ENSP00000451962.2; ENSG00000243279.4.
DR GeneID; 11230; -.
DR KEGG; hsa:11230; -.
DR MANE-Select; ENST00000553851.3; ENSP00000451962.2; NM_007213.3; NP_009144.1.
DR UCSC; uc033ecu.2; human.
DR CTD; 11230; -.
DR DisGeNET; 11230; -.
DR GeneCards; PRAF2; -.
DR HGNC; HGNC:28911; PRAF2.
DR HPA; ENSG00000243279; Tissue enhanced (epididymis).
DR MIM; 300840; gene.
DR neXtProt; NX_O60831; -.
DR OpenTargets; ENSG00000243279; -.
DR PharmGKB; PA134955002; -.
DR VEuPathDB; HostDB:ENSG00000243279; -.
DR eggNOG; KOG4050; Eukaryota.
DR GeneTree; ENSGT00390000008631; -.
DR HOGENOM; CLU_097683_0_0_1; -.
DR InParanoid; O60831; -.
DR OMA; GDPQRWC; -.
DR OrthoDB; 1288023at2759; -.
DR PhylomeDB; O60831; -.
DR TreeFam; TF105479; -.
DR PathwayCommons; O60831; -.
DR SignaLink; O60831; -.
DR BioGRID-ORCS; 11230; 6 hits in 700 CRISPR screens.
DR ChiTaRS; PRAF2; human.
DR GenomeRNAi; 11230; -.
DR Pharos; O60831; Tbio.
DR PRO; PR:O60831; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; O60831; protein.
DR Bgee; ENSG00000243279; Expressed in adenohypophysis and 97 other tissues.
DR ExpressionAtlas; O60831; baseline and differential.
DR Genevisible; O60831; HS.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004895; Prenylated_rab_accept_PRA1.
DR PANTHER; PTHR12859; PTHR12859; 1.
DR Pfam; PF03208; PRA1; 1.
PE 1: Evidence at protein level;
KW Endosome; Membrane; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..178
FT /note="PRA1 family protein 2"
FT /id="PRO_0000220881"
FT TOPO_DOM 1..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..64
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..96
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..122
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 56
FT /note="L -> F (in dbSNP:rs34565429)"
FT /id="VAR_050628"
SQ SEQUENCE 178 AA; 19258 MW; E62695D7DC40550A CRC64;
MSEVRLPPLR ALDDFVLGSA RLAAPDPCDP QRWCHRVINN LLYYQTNYLL CFGIGLALAG
YVRPLHTLLS ALVVAVALGV LVWAAETRAA VRRCRRSHPA ACLAAVLAVG LLVLWVAGGA
CTFLFSIAGP VLLILVHASL RLRNLKNKIE NKIESIGLKR TPMGLLLEAL GQEQEAGS
