PRAF3_BOVIN
ID PRAF3_BOVIN Reviewed; 188 AA.
AC Q5E9M1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=PRA1 family protein 3;
DE AltName: Full=ADP-ribosylation factor-like protein 6-interacting protein 5;
DE Short=ARL-6-interacting protein 5;
DE Short=Aip-5;
GN Name=ARL6IP5; Synonyms=PRAF3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulates intracellular concentrations of taurine and
CC glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport
CC activity by decreasing its affinity for glutamate in a PKC activity-
CC dependent manner. Plays a role in the retention of SLC1A1/EAAC1 in the
CC endoplasmic reticulum. {ECO:0000250|UniProtKB:Q8R5J9,
CC ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SUBUNIT: Homodimer. Heterodimer with ARL6IP1. Forms multimers.
CC Interacts with ARL6. Interacts with prenylated RAB1A and RAB3A.
CC Interacts with SLC1A1/EAAC1. Interacts with RTN2 (via first
CC transmembrane domain). Does not interact with VAMP1, VAMP2 or VAMP3.
CC {ECO:0000250|UniProtKB:Q8R5J9, ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass
CC membrane protein {ECO:0000255}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9ES40}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q9ES40}. Note=Also exists as a soluble form in
CC the cytoplasm. Associated with microtubules.
CC {ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SIMILARITY: Belongs to the PRA1 family. {ECO:0000305}.
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DR EMBL; BT020899; AAX08916.1; -; mRNA.
DR EMBL; BC102757; AAI02758.1; -; mRNA.
DR RefSeq; NP_001014891.1; NM_001014891.1.
DR AlphaFoldDB; Q5E9M1; -.
DR SMR; Q5E9M1; -.
DR STRING; 9913.ENSBTAP00000028661; -.
DR PaxDb; Q5E9M1; -.
DR PeptideAtlas; Q5E9M1; -.
DR PRIDE; Q5E9M1; -.
DR Ensembl; ENSBTAT00000028661; ENSBTAP00000028661; ENSBTAG00000021506.
DR GeneID; 509977; -.
DR KEGG; bta:509977; -.
DR CTD; 10550; -.
DR VEuPathDB; HostDB:ENSBTAG00000021506; -.
DR VGNC; VGNC:26151; ARL6IP5.
DR eggNOG; KOG4050; Eukaryota.
DR GeneTree; ENSGT00390000008631; -.
DR HOGENOM; CLU_097683_0_0_1; -.
DR InParanoid; Q5E9M1; -.
DR OMA; QMKKRYP; -.
DR OrthoDB; 1288023at2759; -.
DR TreeFam; TF105479; -.
DR Reactome; R-BTA-210500; Glutamate Neurotransmitter Release Cycle.
DR Proteomes; UP000009136; Chromosome 22.
DR Bgee; ENSBTAG00000021506; Expressed in cardiac atrium and 104 other tissues.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:Ensembl.
DR GO; GO:0008631; P:intrinsic apoptotic signaling pathway in response to oxidative stress; IEA:Ensembl.
DR GO; GO:0098712; P:L-glutamate import across plasma membrane; IEA:Ensembl.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; ISS:AgBase.
DR GO; GO:0007611; P:learning or memory; IEA:Ensembl.
DR GO; GO:0002037; P:negative regulation of L-glutamate import across plasma membrane; ISS:UniProtKB.
DR GO; GO:0010917; P:negative regulation of mitochondrial membrane potential; IEA:Ensembl.
DR GO; GO:0051051; P:negative regulation of transport; IBA:GO_Central.
DR GO; GO:0036475; P:neuron death in response to oxidative stress; IEA:Ensembl.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IEA:Ensembl.
DR GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Ensembl.
DR GO; GO:0032874; P:positive regulation of stress-activated MAPK cascade; IEA:Ensembl.
DR GO; GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl.
DR GO; GO:0015031; P:protein transport; IEA:Ensembl.
DR InterPro; IPR004895; Prenylated_rab_accept_PRA1.
DR PANTHER; PTHR12859; PTHR12859; 1.
DR Pfam; PF03208; PRA1; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cell membrane; Cytoplasm; Cytoskeleton; Endoplasmic reticulum;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..188
FT /note="PRA1 family protein 3"
FT /id="PRO_0000256847"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 103..117
FT /note="Required for homodimer formation and heterodimer
FT formation with ARL6IP1"
FT /evidence="ECO:0000250|UniProtKB:Q8R5J9"
FT REGION 136..188
FT /note="Targeting to endoplasmic reticulum membrane"
FT /evidence="ECO:0000250|UniProtKB:Q9ES40"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O75915"
SQ SEQUENCE 188 AA; 21666 MW; DDCB3D3CC815AF4F CRC64;
MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISVVGF
LSPFNMILGG IVVVLVFTGF VWAAHNKDIL RRMKKQYPTA FVMVVMLASY FLISLFGGVM
VFVFGITFPL LLMFIHASLR LRNLKNKLEN KMEEIGLKRT PMGIVLDALE QQEETITKFS
DYISKMKE
