PRAF3_PIG
ID PRAF3_PIG Reviewed; 188 AA.
AC Q56P28;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=PRA1 family protein 3;
DE AltName: Full=ADP-ribosylation factor-like protein 6-interacting protein 5;
DE Short=ARL-6-interacting protein 5;
DE Short=Aip-5;
GN Name=ARL6IP5; Synonyms=PRAF3;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Meishan; TISSUE=Muscle;
RA Liu G.Y., Xiong Z.Y.;
RT "Isolation and prediction of one novel swine gene that is differentially
RT expressed in the longissimus dorsi muscle tissues from large white meishan
RT cross combination.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulates intracellular concentrations of taurine and
CC glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport
CC activity by decreasing its affinity for glutamate in a PKC activity-
CC dependent manner. Plays a role in the retention of SLC1A1/EAAC1 in the
CC endoplasmic reticulum. {ECO:0000250|UniProtKB:Q8R5J9,
CC ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SUBUNIT: Homodimer. Heterodimer with ARL6IP1. Forms multimers.
CC Interacts with ARL6. Interacts with prenylated RAB1A and RAB3A.
CC Interacts with SLC1A1/EAAC1. Interacts with RTN2 (via first
CC transmembrane domain). Does not interact with VAMP1, VAMP2 or VAMP3.
CC {ECO:0000250|UniProtKB:Q8R5J9, ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass
CC membrane protein {ECO:0000255}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9ES40}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q9ES40}. Note=Also exists as a soluble form in
CC the cytoplasm. Associated with microtubules.
CC {ECO:0000250|UniProtKB:Q9ES40}.
CC -!- SIMILARITY: Belongs to the PRA1 family. {ECO:0000305}.
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DR EMBL; AY864608; AAX77004.1; -; mRNA.
DR RefSeq; NP_001041538.1; NM_001048073.1.
DR AlphaFoldDB; Q56P28; -.
DR SMR; Q56P28; -.
DR STRING; 9823.ENSSSCP00000012264; -.
DR PaxDb; Q56P28; -.
DR PeptideAtlas; Q56P28; -.
DR PRIDE; Q56P28; -.
DR Ensembl; ENSSSCT00000037977; ENSSSCP00000036690; ENSSSCG00000033154.
DR Ensembl; ENSSSCT00005033908; ENSSSCP00005020701; ENSSSCG00005021411.
DR Ensembl; ENSSSCT00015032943; ENSSSCP00015013086; ENSSSCG00015024811.
DR Ensembl; ENSSSCT00025009137; ENSSSCP00025003608; ENSSSCG00025006884.
DR Ensembl; ENSSSCT00030053141; ENSSSCP00030024249; ENSSSCG00030038167.
DR Ensembl; ENSSSCT00040004184; ENSSSCP00040001318; ENSSSCG00040003334.
DR Ensembl; ENSSSCT00045027357; ENSSSCP00045018904; ENSSSCG00045016085.
DR Ensembl; ENSSSCT00050028417; ENSSSCP00050011762; ENSSSCG00050021082.
DR Ensembl; ENSSSCT00055018542; ENSSSCP00055014596; ENSSSCG00055009514.
DR Ensembl; ENSSSCT00060033026; ENSSSCP00060014164; ENSSSCG00060024350.
DR Ensembl; ENSSSCT00065106006; ENSSSCP00065047105; ENSSSCG00065076702.
DR Ensembl; ENSSSCT00070045812; ENSSSCP00070038615; ENSSSCG00070023018.
DR GeneID; 595127; -.
DR KEGG; ssc:595127; -.
DR CTD; 10550; -.
DR VGNC; VGNC:103904; ARL6IP5.
DR eggNOG; KOG4050; Eukaryota.
DR GeneTree; ENSGT00390000008631; -.
DR HOGENOM; CLU_097683_0_0_1; -.
DR InParanoid; Q56P28; -.
DR OMA; QTNYFVV; -.
DR OrthoDB; 1288023at2759; -.
DR TreeFam; TF105479; -.
DR Reactome; R-SSC-210500; Glutamate Neurotransmitter Release Cycle.
DR Proteomes; UP000008227; Chromosome 13.
DR Proteomes; UP000314985; Chromosome 13.
DR Bgee; ENSSSCG00000033154; Expressed in hypothalamus and 46 other tissues.
DR ExpressionAtlas; Q56P28; baseline and differential.
DR Genevisible; Q56P28; SS.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; ISS:AgBase.
DR GO; GO:0002037; P:negative regulation of L-glutamate import across plasma membrane; ISS:UniProtKB.
DR GO; GO:0051051; P:negative regulation of transport; IBA:GO_Central.
DR InterPro; IPR004895; Prenylated_rab_accept_PRA1.
DR PANTHER; PTHR12859; PTHR12859; 1.
DR Pfam; PF03208; PRA1; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cell membrane; Cytoplasm; Cytoskeleton; Endoplasmic reticulum;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..188
FT /note="PRA1 family protein 3"
FT /id="PRO_0000256849"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 103..117
FT /note="Required for homodimer formation and heterodimer
FT formation with ARL6IP1"
FT /evidence="ECO:0000250|UniProtKB:Q8R5J9"
FT REGION 136..188
FT /note="Targeting to endoplasmic reticulum membrane"
FT /evidence="ECO:0000250|UniProtKB:Q9ES40"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O75915"
SQ SEQUENCE 188 AA; 21541 MW; C7C1A312F73DA58E CRC64;
MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISVVGF
LSPFNMILGG IVVVLVFTGF VWAAHNKDIL RRLKKQYPTV FVMVVMLASY FLISMFGGVM
VFVFGITFPL LLMFIHASLR LRNLKNKLEN KIEGIGLKRT PMGIVLDALE QQEENISKFA
DYISKVNE
