PRAL1_MOUSE
ID PRAL1_MOUSE Reviewed; 458 AA.
AC Q99MW3;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Preferentially expressed antigen in melanoma-like protein 1 {ECO:0000312|EMBL:AAI31996.1};
DE Short=Prame-like 1 {ECO:0000303|PubMed:23565261};
GN Name=Pramel1 {ECO:0000312|MGI:MGI:1890541};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:AAK31957.1};
RN [1] {ECO:0000312|EMBL:AAK31957.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAK31957.1};
RX PubMed=11279525; DOI=10.1038/86927;
RA Wang P.J., McCarrey J.R., Yang F., Page D.C.;
RT "An abundance of X-linked genes expressed in spermatogonia.";
RL Nat. Genet. 27:422-426(2001).
RN [2] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:EDL13429.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AAI31996.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain {ECO:0000312|EMBL:AAI31996.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=23565261; DOI=10.1371/journal.pone.0060611;
RA Mistry B.V., Zhao Y., Chang T.C., Yasue H., Chiba M., Oatley J., Diaz F.,
RA Liu W.S.;
RT "Differential expression of PRAMEL1, a cancer/testis antigen, during
RT spermatogenesis in the mouse.";
RL PLoS ONE 8:E60611-E60611(2013).
CC -!- FUNCTION: May play a role in acrosome development and also in sperm
CC maturation and motility. {ECO:0000303|PubMed:23565261}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23565261}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:23565261}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:23565261}. Note=Located in the cytoplasm of
CC spermatocytes and in the acrosomal vesicle region of round, elongating
CC and elongated spermatids. Detected in the main piece of the flagellum
CC in testicular spermatozoa. Detected also in the connecting piece,
CC middle piece and cytoplasmic droplets of epididymal spermatozoa.
CC {ECO:0000269|PubMed:23565261}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis (at protein
CC level). {ECO:0000269|PubMed:23565261}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the spermatogenic cells during early
CC spermatogenesis (at protein level). Expressed postnatally from week 1
CC onwards with a slight increase in expression levels until week 3 (at
CC protein level). {ECO:0000269|PubMed:23565261}.
CC -!- SIMILARITY: Belongs to the PRAME family. {ECO:0000305}.
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DR EMBL; AF285578; AAK31957.1; -; mRNA.
DR EMBL; AL611930; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466615; EDL13429.1; -; Genomic_DNA.
DR EMBL; BC131995; AAI31996.1; -; mRNA.
DR EMBL; BC132399; AAI32400.1; -; mRNA.
DR CCDS; CCDS18888.1; -.
DR RefSeq; NP_113554.1; NM_031377.2.
DR AlphaFoldDB; Q99MW3; -.
DR SMR; Q99MW3; -.
DR IntAct; Q99MW3; 1.
DR STRING; 10090.ENSMUSP00000043718; -.
DR iPTMnet; Q99MW3; -.
DR PhosphoSitePlus; Q99MW3; -.
DR PaxDb; Q99MW3; -.
DR PRIDE; Q99MW3; -.
DR ProteomicsDB; 289833; -.
DR Ensembl; ENSMUST00000037419; ENSMUSP00000043718; ENSMUSG00000041805.
DR GeneID; 83491; -.
DR KEGG; mmu:83491; -.
DR UCSC; uc008vqc.2; mouse.
DR CTD; 83491; -.
DR MGI; MGI:1890541; Pramel1.
DR VEuPathDB; HostDB:ENSMUSG00000041805; -.
DR eggNOG; ENOG502QWSJ; Eukaryota.
DR GeneTree; ENSGT01030000234531; -.
DR HOGENOM; CLU_039635_2_1_1; -.
DR InParanoid; Q99MW3; -.
DR OMA; YSTCILN; -.
DR OrthoDB; 563553at2759; -.
DR PhylomeDB; Q99MW3; -.
DR TreeFam; TF332708; -.
DR BioGRID-ORCS; 83491; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q99MW3; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q99MW3; protein.
DR Bgee; ENSMUSG00000041805; Expressed in spermatid and 7 other tissues.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:MGI.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISO:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IEA:InterPro.
DR GO; GO:0045596; P:negative regulation of cell differentiation; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR026271; PRAME_family.
DR PIRSF; PIRSF038286; PRAME; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoplasmic vesicle; Flagellum;
KW Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..458
FT /note="Preferentially expressed antigen in melanoma-like
FT protein 1"
FT /id="PRO_0000438837"
FT REPEAT 99..126
FT /note="LRR 1; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 176..200
FT /note="LRR 2; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 201..227
FT /note="LRR 3; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 228..263
FT /note="LRR 4; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 264..289
FT /note="LRR 5"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 290..321
FT /note="LRR 6"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 322..340
FT /note="LRR 7"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 346..373
FT /note="LRR 8"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 374..398
FT /note="LRR 9"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
SQ SEQUENCE 458 AA; 52402 MW; 94F76C7A9BC25DB9 CRC64;
MSCKTPPTLQ ELAENSLLKN QDLAISALDD IPSLFFPSLF KKACRNRYVG IIKAMVQAWP
FPCLPLGAMI SRKTAYRRIL EIILYGLDAL LSQKVPHSRC KLQVLDLRVM PLKLWNRLPV
FGTAGCSENP AVVGHSGTEV KQPVKVLVDL VLKESPLDST ESFLVQWVDN RNGLVSLCCC
KLQIWAMSMY YHRKLLEILD LDSVQELRMY CISNPVCLLN FAPYLGRMRN LRCLILSHLW
QTFSMTPVEK QQVITQFTSQ FLKLKCLQIL HLDTVFFLEG HLDELFWWLK TPLETLSVID
CNLSKSDWFH ISEFQCTSQL KHLNLKWVKL THLSPEPLRV LLLKSASTLT SLDLEGCQMM
DSQLSAILPA LRCCTQLTKF NFHGNYISMP ILRELAYNVV KQKSQQSKIR FIPSCSHHSG
LEFEAISQPH IVFVDVDRTT GEQEQVLFYA ICSGEYVL
