PRAM9_HUMAN
ID PRAM9_HUMAN Reviewed; 478 AA.
AC P0DUQ2; B2RPE1; Q08EQ1; Q5VWM5;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 1.
DT 03-AUG-2022, entry version 4.
DE RecName: Full=PRAME family member 9 {ECO:0000305};
GN Name=PRAMEF9 {ECO:0000303|PubMed:26138980, ECO:0000312|HGNC:HGNC:27996};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [2]
RP FUNCTION, PATHWAY, AND IDENTIFICATION IN A CRL2 E3 UBIQUITIN-PROTEIN LIGASE
RP COMPLEX.
RX PubMed=26138980; DOI=10.1126/science.aab0515;
RA Lin H.C., Ho S.C., Chen Y.Y., Khoo K.H., Hsu P.H., Yen H.C.;
RT "SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced
RT by failed UGA/Sec decoding.";
RL Science 349:91-95(2015).
CC -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC ubiquitin-protein ligase complex, which mediates ubiquitination of
CC target proteins, leading to their degradation (PubMed:26138980). The
CC CRL2(PRAMEF9) complex mediates ubiquitination and degradation of
CC truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec
CC decoding (PubMed:26138980). {ECO:0000269|PubMed:26138980}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000269|PubMed:26138980}.
CC -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex, composed of
CC CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter
CC PRAMEF9. {ECO:0000269|PubMed:26138980}.
CC -!- SIMILARITY: Belongs to the PRAME family. {ECO:0000305}.
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DR EMBL; BX470067; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001091846.1; NM_001098376.2.
DR iPTMnet; Q5VWM5; -.
DR PeptideAtlas; P0DUQ2; -.
DR GeneID; 653619; -.
DR KEGG; hsa:653619; -.
DR MANE-Select; ENST00000376152.2; ENSP00000365322.1; NM_001098376.3; NP_001091846.1.
DR CTD; 653619; -.
DR HGNC; HGNC:27996; PRAMEF9.
DR neXtProt; NX_P0DUQ2; -.
DR OpenTargets; ENSG00000204501; -.
DR GeneTree; ENSGT01030000234531; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000005640; Chromosome 1.
DR Bgee; ENSG00000204501; Expressed in liver and 3 other tissues.
DR Genevisible; Q5VWM5; HS.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IDA:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IEA:InterPro.
DR GO; GO:0045596; P:negative regulation of cell differentiation; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:InterPro.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR026271; PRAME_family.
DR PIRSF; PIRSF038286; PRAME; 1.
PE 1: Evidence at protein level;
KW Leucine-rich repeat; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..478
FT /note="PRAME family member 9"
FT /id="PRO_0000156983"
FT REPEAT 99..126
FT /note="LRR 1; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 181..205
FT /note="LRR 2; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 206..232
FT /note="LRR 3; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 233..268
FT /note="LRR 4; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 269..294
FT /note="LRR 5"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 295..326
FT /note="LRR 6"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 327..347
FT /note="LRR 7"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 351..378
FT /note="LRR 8"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 379..403
FT /note="LRR 9"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
SQ SEQUENCE 478 AA; 55420 MW; 01C7079F4D65BF10 CRC64;
MKMSIRTPPR LLELAGRSLL RDQALAMSTL EELPTELFPP LFMEAFSRRR CEALKLMVQA
WPFRRLPLRP LIKMPCLEAF QAVLDGLDAL LTQGVRPRRW KLQVLDLQDV CENFWMVWSE
AMAHGCFLNA KRNKKPVQDC PRMRGRQPLT VFVELWLKNR TLDEYLTYLL LWVKQRKDLL
HLCCKKLKIL GMPFRNIRSI LKMVNLDCIQ EVEVNCKWVL PILTQFTPYL GHMRNLQKLV
LSHMDVSRYV SPEQKKEIVT QFTTQFLKLR CLQKLYMNSV SFLEGHLDQL LSCLKTSLKV
LTITNCVLLE SDLKHLSQCP SISQLKTLDL SGIRLTNYSL VPLQILLEKV AATLEYLDLD
DCGIIDSQVN AILPALSRCF ELNTFSFCGN PICMATLENL LSHTIILKNL CVELYPAPRE
SYGADGTLCW SRFAQIRAEL MNRVRDLRHP KRILFCTDYC PDCGNRSFYD LEADQYCC
