PRA_MYCLE
ID PRA_MYCLE Reviewed; 249 AA.
AC P41484;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Proline-rich antigen {ECO:0000303|PubMed:1688422};
DE Short=Pra {ECO:0000303|PubMed:1688422};
DE AltName: Full=36 kDa antigen;
GN Name=ag36; Synonyms=pra {ECO:0000303|PubMed:1688422};
GN OrderedLocusNames=ML2395;
OS Mycobacterium leprae (strain TN).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=272631;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DOMAIN.
RC STRAIN=5-3;
RX PubMed=1688422; DOI=10.1128/iai.58.1.80-87.1990;
RA Thole J.E.R., Stabel L.F.E.M., Suykerbuyk M.E.G., de Wit M.Y.L.,
RA Klatser P.R., Kolk A.H.J., Hartskeerl R.A.;
RT "A major immunogenic 36,000-molecular-weight antigen from Mycobacterium
RT leprae contains an immunoreactive region of proline-rich repeats.";
RL Infect. Immun. 58:80-87(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Smith D.R., Robison K.;
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TN;
RX PubMed=11234002; DOI=10.1038/35059006;
RA Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA Barrell B.G.;
RT "Massive gene decay in the leprosy bacillus.";
RL Nature 409:1007-1011(2001).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DOMAIN: Its N-terminus, which contains the proline-rich repeats, is
CC highly immunoreactive. {ECO:0000269|PubMed:1688422}.
CC -!- SIMILARITY: Belongs to the mycobacterial Pra family. {ECO:0000305}.
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DR EMBL; X65546; CAA46515.1; -; Genomic_DNA.
DR EMBL; U15183; AAA63035.1; -; Genomic_DNA.
DR EMBL; AL583925; CAC31911.1; -; Genomic_DNA.
DR PIR; A41497; A41497.
DR RefSeq; NP_302551.1; NC_002677.1.
DR RefSeq; WP_010908871.1; NC_002677.1.
DR AlphaFoldDB; P41484; -.
DR STRING; 272631.ML2395; -.
DR EnsemblBacteria; CAC31911; CAC31911; CAC31911.
DR KEGG; mle:ML2395; -.
DR PATRIC; fig|272631.5.peg.4608; -.
DR Leproma; ML2395; -.
DR eggNOG; COG1714; Bacteria.
DR HOGENOM; CLU_053152_1_0_11; -.
DR OMA; KSVMKFK; -.
DR Proteomes; UP000000806; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR010432; RDD.
DR Pfam; PF06271; RDD; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..249
FT /note="Proline-rich antigen"
FT /id="PRO_0000058566"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 34..43
FT /note="1-1; approximate"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REPEAT 46..55
FT /note="1-2"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REPEAT 56..65
FT /note="1-3"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REPEAT 66..75
FT /note="1-4"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REPEAT 76..85
FT /note="1-5; approximate"
FT /evidence="ECO:0000303|PubMed:1688422"
FT DOMAIN 99..242
FT /note="RDD"
FT /evidence="ECO:0000255"
FT REPEAT 101..123
FT /note="2-1"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REPEAT 134..156
FT /note="2-2"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REGION 1..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..85
FT /note="5 X 10 AA tandem repeats of [PV]-G-G-S-Y-P-P-P-P-P"
FT /evidence="ECO:0000303|PubMed:1688422"
FT REGION 101..156
FT /note="2 X 23 AA approximate repeats"
FT /evidence="ECO:0000303|PubMed:1688422"
FT COMPBIAS 1..24
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..87
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 211..212
FT /note="QL -> HV (in Ref. 1; CAA46515)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 26295 MW; 28E565587E1570DA CRC64;
MTDQPPPSGS NPTPAPPPPG SSGGYEPSFA PSELGSAYPP PTAPPVGGSY PPPPPPGGSY
PPPPPPGGSY PPPPPSTGAY APPPPGPAIR SLPKEAYTFW VTRVLAYVID NIPATVLLGI
GMLIQTLTKQ EACVTDITQY NVNQYCATQP TGIGMLAFWF AWLMATAYLV WNYGYRQGAT
GSSIGKTVMK FKVISEATGQ PIGFGMSVVR QLAHFVDAVI CCIGFLFPLW DSKRQTLADK
IMTTVCLPI
