PRCA1_HUMAN
ID PRCA1_HUMAN Reviewed; 364 AA.
AC Q8NCQ7; B4DX95; G5E9R8; Q6PKN3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Protein PROCA1 {ECO:0000305};
DE AltName: Full=Protein interacting with cyclin A1 {ECO:0000303|PubMed:15159402};
GN Name=PROCA1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-81 (ISOFORM 3), AND TISSUE SPECIFICITY.
RX PubMed=15159402; DOI=10.1074/jbc.m401708200;
RA Diederichs S., Baeumer N., Ji P., Metzelder S.K., Idos G.E., Cauvet T.,
RA Wang W., Moeller M., Pierschalski S., Gromoll J., Schrader M.G.,
RA Koeffler H.P., Berdel W.E., Serve H., Mueller-Tidow C.;
RT "Identification of interaction partners and substrates of the cyclin A1-
RT CDK2 complex.";
RL J. Biol. Chem. 279:33727-33741(2004).
CC -!- INTERACTION:
CC Q8NCQ7-2; P55212: CASP6; NbExp=3; IntAct=EBI-25836043, EBI-718729;
CC Q8NCQ7-2; O00291: HIP1; NbExp=3; IntAct=EBI-25836043, EBI-473886;
CC Q8NCQ7-2; P13473-2: LAMP2; NbExp=3; IntAct=EBI-25836043, EBI-21591415;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8NCQ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NCQ7-2; Sequence=VSP_033988;
CC Name=3;
CC IsoId=Q8NCQ7-3; Sequence=VSP_053931, VSP_053932;
CC -!- TISSUE SPECIFICITY: High expressed in testis.
CC {ECO:0000269|PubMed:15159402}.
CC -!- SIMILARITY: Belongs to the PROCA1 family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to interact with CCNA1 and found in a
CC complex with CCNA1 and CDK2 (PubMed:15159402). However the amino acid
CC sequence described in this paper contains several frameshifts and a
CC wrong choice of frame, so results from this paper need to be validated.
CC {ECO:0000305|PubMed:15159402}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT09159.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
CC Sequence=AAT09159.1; Type=Frameshift; Evidence={ECO:0000305};
CC -!- SEQUENCE CAUTION: [Isoform 3]:
CC Sequence=AAT09159.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK301868; BAG63307.1; -; mRNA.
DR EMBL; AC010761; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471159; EAW51125.1; -; Genomic_DNA.
DR EMBL; CH471159; EAW51126.1; -; Genomic_DNA.
DR EMBL; BC029574; AAH29574.1; -; mRNA.
DR EMBL; AY601916; AAT09159.1; ALT_SEQ; mRNA.
DR CCDS; CCDS11239.1; -. [Q8NCQ7-2]
DR RefSeq; NP_001291878.1; NM_001304949.1.
DR RefSeq; NP_001291880.1; NM_001304951.1.
DR RefSeq; NP_001291881.1; NM_001304952.1. [Q8NCQ7-3]
DR RefSeq; NP_001291882.1; NM_001304953.1.
DR RefSeq; NP_001291883.1; NM_001304954.1.
DR RefSeq; NP_689678.1; NM_152465.2. [Q8NCQ7-2]
DR AlphaFoldDB; Q8NCQ7; -.
DR BioGRID; 127029; 4.
DR IntAct; Q8NCQ7; 4.
DR STRING; 9606.ENSP00000301039; -.
DR iPTMnet; Q8NCQ7; -.
DR PhosphoSitePlus; Q8NCQ7; -.
DR BioMuta; PROCA1; -.
DR DMDM; 189037421; -.
DR MassIVE; Q8NCQ7; -.
DR PeptideAtlas; Q8NCQ7; -.
DR PRIDE; Q8NCQ7; -.
DR ProteomicsDB; 34024; -.
DR ProteomicsDB; 72923; -. [Q8NCQ7-1]
DR ProteomicsDB; 72924; -. [Q8NCQ7-2]
DR Antibodypedia; 14279; 78 antibodies from 14 providers.
DR DNASU; 147011; -.
DR Ensembl; ENST00000301039.6; ENSP00000301039.2; ENSG00000167525.15. [Q8NCQ7-2]
DR Ensembl; ENST00000439862.7; ENSP00000411400.4; ENSG00000167525.15. [Q8NCQ7-1]
DR GeneID; 147011; -.
DR KEGG; hsa:147011; -.
DR UCSC; uc002hca.2; human. [Q8NCQ7-1]
DR CTD; 147011; -.
DR DisGeNET; 147011; -.
DR GeneCards; PROCA1; -.
DR HGNC; HGNC:28600; PROCA1.
DR HPA; ENSG00000167525; Tissue enriched (testis).
DR MIM; 617376; gene.
DR neXtProt; NX_Q8NCQ7; -.
DR OpenTargets; ENSG00000167525; -.
DR PharmGKB; PA165432492; -.
DR VEuPathDB; HostDB:ENSG00000167525; -.
DR eggNOG; ENOG502QTYI; Eukaryota.
DR GeneTree; ENSGT00940000162235; -.
DR InParanoid; Q8NCQ7; -.
DR OMA; FSDCGHH; -.
DR OrthoDB; 1306595at2759; -.
DR PhylomeDB; Q8NCQ7; -.
DR TreeFam; TF350402; -.
DR PathwayCommons; Q8NCQ7; -.
DR SignaLink; Q8NCQ7; -.
DR BioGRID-ORCS; 147011; 73 hits in 1079 CRISPR screens.
DR ChiTaRS; PROCA1; human.
DR GenomeRNAi; 147011; -.
DR Pharos; Q8NCQ7; Tbio.
DR PRO; PR:Q8NCQ7; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q8NCQ7; protein.
DR Bgee; ENSG00000167525; Expressed in left testis and 106 other tissues.
DR ExpressionAtlas; Q8NCQ7; baseline and differential.
DR Genevisible; Q8NCQ7; HS.
DR GO; GO:0030332; F:cyclin binding; IPI:UniProtKB.
DR GO; GO:0004623; F:phospholipase A2 activity; IEA:InterPro.
DR GO; GO:0050482; P:arachidonic acid secretion; IEA:InterPro.
DR GO; GO:0006644; P:phospholipid metabolic process; IEA:InterPro.
DR Gene3D; 1.20.90.10; -; 1.
DR InterPro; IPR016090; PLipase_A2_dom.
DR InterPro; IPR036444; PLipase_A2_dom_sf.
DR Pfam; PF05826; Phospholip_A2_2; 1.
DR SUPFAM; SSF48619; SSF48619; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..364
FT /note="Protein PROCA1"
FT /id="PRO_0000336063"
FT REGION 172..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..309
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..337
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 276
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT MOD_RES 308
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT MOD_RES 318
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT MOD_RES 319
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT MOD_RES 364
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4V7B4"
FT VAR_SEQ 1..30
FT /note="MWVRTTLTIERWTKEKTEPKARSWDEALSD -> MSITY (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_053931"
FT VAR_SEQ 27..58
FT /note="ALSDVNRLPSWERGHLLAGVASSTDVSTFSEG -> SRCR (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033988"
FT VAR_SEQ 58
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_053932"
FT VARIANT 245
FT /note="D -> A (in dbSNP:rs1077127)"
FT /id="VAR_043476"
FT VARIANT 320
FT /note="E -> K (in dbSNP:rs3744637)"
FT /id="VAR_043477"
FT CONFLICT 89
FT /note="H -> Y (in Ref. 1; BAG63307)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 364 AA; 40506 MW; BAED65CDC427C7A3 CRC64;
MWVRTTLTIE RWTKEKTEPK ARSWDEALSD VNRLPSWERG HLLAGVASST DVSTFSEGGD
CKEPDKCCWR HKQCTGHIIY PFASDCVRHS LHLHSVNHCN CNSRLKDSSE DSSSSRGAGP
TCSHVIESPC FELTPEEEHV ERFRYGWCKS YRPVSVAVIH HPLYHECGAD DLNEEEEEEE
EESKPPIPTQ VGPATASPDL GTSMATGTPD STAPITIWRS ESPTGKGQGS KVIKKVKKKK
EKEKDKEEMD EKAKLKKKAK KGQLTKKKSP VKLEPSPPDV SRSLSARQLA RMSESSPESR
EELESEDSYN GRGQGELSSE DIVESSSPRK RENTVQAKKT GAKPSQARKV NKRKSPPGSN
PNLS
